##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062692_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248612
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.4635053818802	32.0	32.0	32.0	32.0	32.0
2	31.323520184061913	32.0	32.0	32.0	32.0	32.0
3	31.440666580856917	32.0	32.0	32.0	32.0	32.0
4	31.587932199572023	32.0	32.0	32.0	32.0	32.0
5	31.508205557253874	32.0	32.0	32.0	32.0	32.0
6	35.06425675349541	36.0	36.0	36.0	36.0	36.0
7	35.020783389377826	36.0	36.0	36.0	36.0	36.0
8	34.97172702846202	36.0	36.0	36.0	36.0	36.0
9	34.92861567422329	36.0	36.0	36.0	32.0	36.0
10	34.92783131948578	36.0	36.0	36.0	32.0	36.0
11	34.99738950654031	36.0	36.0	36.0	36.0	36.0
12	34.90942110598041	36.0	36.0	36.0	32.0	36.0
13	34.89675880488472	36.0	36.0	36.0	32.0	36.0
14	34.866892989879815	36.0	36.0	36.0	32.0	36.0
15	34.91184254983669	36.0	36.0	36.0	32.0	36.0
16	34.889357714028286	36.0	36.0	36.0	32.0	36.0
17	34.87059353530803	36.0	36.0	36.0	32.0	36.0
18	34.88965134426335	36.0	36.0	36.0	32.0	36.0
19	34.754227470918536	36.0	36.0	36.0	32.0	36.0
20	34.616663716956545	36.0	36.0	36.0	32.0	36.0
21	34.52116551091661	36.0	36.0	36.0	32.0	36.0
22	34.5692846684794	36.0	36.0	36.0	32.0	36.0
23	34.568210705838815	36.0	36.0	36.0	32.0	36.0
24	34.543312470838096	36.0	36.0	36.0	32.0	36.0
25	34.541735716699115	36.0	36.0	36.0	32.0	36.0
26	34.48230978392033	36.0	36.0	36.0	32.0	36.0
27	34.47457886184094	36.0	36.0	36.0	32.0	36.0
28	34.427449197947	36.0	36.0	36.0	32.0	36.0
29	34.35702218718324	36.0	36.0	36.0	32.0	36.0
30	34.33983476260197	36.0	36.0	36.0	32.0	36.0
31	34.284153620903254	36.0	36.0	36.0	32.0	36.0
32	34.221795408105805	36.0	36.0	36.0	32.0	36.0
33	34.0825060737213	36.0	36.0	36.0	32.0	36.0
34	33.99509275497562	36.0	36.0	36.0	32.0	36.0
35	33.87721027142696	36.0	36.0	36.0	32.0	36.0
36	33.84124660112947	36.0	36.0	36.0	32.0	36.0
37	33.74825028558557	36.0	36.0	36.0	32.0	36.0
38	33.66100590478336	36.0	36.0	36.0	27.0	36.0
39	33.45525960130645	36.0	36.0	36.0	21.0	36.0
40	33.290126783904235	36.0	36.0	36.0	14.0	36.0
41	33.27069087574212	36.0	36.0	36.0	14.0	36.0
42	32.996231074928	36.0	36.0	36.0	14.0	36.0
43	33.00616221260438	36.0	36.0	36.0	14.0	36.0
44	32.92252586359468	36.0	36.0	36.0	14.0	36.0
45	32.57320241983492	36.0	36.0	36.0	14.0	36.0
46	32.63776084822937	36.0	36.0	36.0	14.0	36.0
47	32.37651038566119	36.0	36.0	36.0	14.0	36.0
48	32.31995639792126	36.0	36.0	36.0	14.0	36.0
49	32.303183273534664	36.0	36.0	36.0	14.0	36.0
50	31.56945360642286	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	6.0
21	22.0
22	50.0
23	138.0
24	353.0
25	979.0
26	1811.0
27	3603.0
28	5995.0
29	8405.0
30	11353.0
31	14891.0
32	20430.0
33	25085.0
34	40960.0
35	114527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.03021952602975	17.581391980883495	12.618422164204022	21.769966328882738
2	14.061205280644876	22.59599526962342	36.67248578071326	26.670313669018448
3	20.113190243274552	29.25127107735873	27.311269146608314	23.3242695327584
4	12.153476099303333	15.914356507328689	32.92359178157128	39.0085756117967
5	12.769697359741283	39.67145592328609	30.940582111885185	16.618264605087447
6	32.249578659029574	32.68841684398518	18.857170438958857	16.204834058026393
7	28.384792367222822	32.72327964860908	20.34093285923447	18.550995124933632
8	32.19554969188937	27.82247035541325	20.744372757549918	19.237607195147458
9	29.98688719772175	13.916866442488699	18.789117178575452	37.3071291812141
10	18.45526362363844	26.567905008607788	29.019918588000582	25.95691277975319
11	35.77059836210641	22.897527070294274	19.38482454587872	21.947050021720592
12	24.980290573262756	26.258989911991375	27.21992502373176	21.540794491014108
13	32.44694544108893	19.81280066931604	25.73367335446398	22.00658053513105
14	26.419883191479094	19.126993065499654	25.914678293887665	28.538445449133587
15	27.087590301353114	24.347577751677314	27.618940356861295	20.945891590108282
16	24.704760832140042	26.00156066481103	26.310475761427444	22.983202741621483
17	22.16425594902901	26.128666355606327	27.2875002011166	24.419577494248067
18	24.597364568081993	22.873795311569836	30.64453847762779	21.884301642720384
19	23.683892973790485	28.377552169645874	27.51918652357891	20.41936833298473
20	25.266680610750893	20.937042459736457	31.069698968674082	22.726577960838576
21	26.870786607243414	24.362862613228646	25.23409972165463	23.532251057873314
22	24.339130854504205	24.993564268820492	26.50113429762039	24.166170579054914
23	21.605956269206636	25.286390037488133	27.314852058629512	25.792801634675723
24	21.482470677199814	28.916142422731	26.991456566859203	22.60993033320998
25	24.7747494087172	24.925989091435653	25.597316300098143	24.701945199749005
26	21.596302672437375	25.91065596190047	27.883207568419866	24.60983379724229
27	24.47830354126108	27.27583543835374	27.365935674866858	20.87992534551832
28	22.47598667803646	24.69872733415925	28.507875726030925	24.317410261773365
29	20.525960130645345	27.76977780638103	29.891960162824	21.81230190014963
30	23.475938410052613	26.80602706224961	28.63498141682622	21.083053110871557
31	20.91934419899281	27.871945038855728	26.836194552153557	24.372516209997908
32	20.809936768941164	28.109262626100108	27.518382057181473	23.56241854777726
33	20.791031808601353	27.490627966469837	27.34823741412321	24.370102810805594
34	20.661914951812463	28.77174070439078	28.560568275063154	22.00577606873361
35	23.52863095908484	27.71185622576545	27.30439399546281	21.455118819686902
36	21.76242498350844	29.6522291763873	26.500329831222952	22.085016008881308
37	23.566440879764453	29.34250961337345	26.888887101185784	20.202162405676315
38	22.03232345984908	29.098354061750843	25.434009621418113	23.435312856981962
39	21.470805914436955	28.891606197609125	27.305600695058967	22.331987192894953
40	22.561260116164945	29.39801779479671	25.50359596479655	22.53712612424179
41	19.66759448457838	30.339243479799848	27.014786092384924	22.978375943236852
42	24.12715395877914	29.734686982124757	26.315704793010795	19.822454266085305
43	22.698019403729504	27.29956719707818	25.06435731179509	24.93805608739723
44	21.54602352259746	28.8389136485769	27.919408556304603	21.695654272521036
45	23.093816871269286	28.68686949946101	25.61742796003411	22.601885669235596
46	20.68484224413946	29.02595208598137	27.737599150483483	22.551606519395683
47	22.241171951361764	28.19867182063545	27.750582234897088	21.809573993105698
48	21.627676861937477	33.143211108072016	23.530642125078437	21.69846990491207
49	20.039660193393722	29.98688719772175	28.07949736939488	21.893955239489646
50	20.358631119978117	33.11264138496935	23.07410744453204	23.45462005052049
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	42.0
1	41.0
2	40.0
3	148.5
4	257.0
5	298.5
6	340.0
7	274.5
8	209.0
9	296.0
10	383.0
11	595.5
12	808.0
13	1311.5
14	1815.0
15	2367.5
16	2920.0
17	3310.0
18	3700.0
19	4046.0
20	4392.0
21	5052.0
22	5712.0
23	6039.5
24	6367.0
25	4818.0
26	3269.0
27	2773.0
28	2277.0
29	2476.0
30	2675.0
31	3337.0
32	3999.0
33	4526.5
34	5054.0
35	5021.0
36	4988.0
37	5340.0
38	5692.0
39	6188.0
40	6684.0
41	7958.5
42	9233.0
43	11083.0
44	12933.0
45	16776.5
46	20620.0
47	23984.0
48	27348.0
49	25801.0
50	24254.0
51	20132.5
52	16011.0
53	14283.0
54	12555.0
55	12787.0
56	13019.0
57	12404.5
58	11790.0
59	11002.5
60	10215.0
61	8948.5
62	7682.0
63	6672.5
64	5663.0
65	5075.5
66	4488.0
67	3948.0
68	3408.0
69	3183.0
70	2958.0
71	2305.0
72	1652.0
73	1465.5
74	1279.0
75	966.0
76	653.0
77	562.5
78	472.0
79	403.0
80	334.0
81	244.0
82	154.0
83	141.0
84	128.0
85	107.5
86	87.0
87	60.5
88	34.0
89	22.5
90	11.0
91	6.0
92	1.0
93	0.5
94	0.0
95	2.0
96	4.0
97	2.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012469229160297975
2	0.002413399192315737
3	0.0016089327948771582
4	0.0
5	0.0
6	4.0223319871928955E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	4.0223319871928955E-4
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	248612.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.462344189927514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.41206238128626	30.747859163366797
2	14.499206290812747	12.313405760025061
3	5.074058994584921	6.463693184041874
4	2.401407806596268	4.078776192962785
5	1.3079277814677293	2.776883981612551
6	0.7964779562748883	2.0292192671420604
7	0.5861395211383641	1.7422200662927558
8	0.4136864543144689	1.4052877287849361
9	0.28808522042222146	1.1009496407039552
>10	1.8760428054596454	15.525886875699847
>50	0.2043511025049377	6.128661832361277
>100	0.1256011654420593	9.496272408938825
>500	0.010965181110021049	3.233431019919041
>1k	0.0039873385854621995	2.9574528781482314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2168	0.8720415748234196	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1907	0.7670587099576851	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1884	0.7578073463871414	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1028	0.41349572828342956	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	994	0.3998197995269738	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	967	0.3889595031615529	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	758	0.30489276462922144	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	751	0.3020771322381864	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	712	0.28639003748813413	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	674	0.2711051759368011	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	633	0.2546136147893102	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	590	0.23731758724438082	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	533	0.2143902949173813	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	520	0.2091612633340305	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	507	0.2039322317506798	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	496	0.1995076665647676	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	463	0.18623397100703104	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	389	0.15646871430180362	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	367	0.14761958392997926	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	359	0.14440171834022492	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	355	0.14279278554534777	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	349	0.14037938635303204	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	343	0.1379659871607163	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	339	0.13635705436583914	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	337	0.13555258796840056	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	301	0.12107219281450614	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	301	0.12107219281450614	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	300	0.12066995961578685	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	285	0.11463646163499752	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	283	0.11383199523755892	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	275	0.11061412964780461	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	274	0.11021189644908533	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	267	0.1073962640580503	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	267	0.1073962640580503	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	253	0.10176499927598023	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	253	0.10176499927598023	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA	252	0.10136276607726095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.022331987192895E-4	0.0
5	0.0	0.0	0.0	4.022331987192895E-4	0.0
6	0.0	0.0	0.0	4.022331987192895E-4	0.0
7	0.0	0.0	0.0	4.022331987192895E-4	0.0
8	0.0	0.0	0.0	4.022331987192895E-4	0.0
9	0.0	0.0	0.0	4.022331987192895E-4	0.0
10	0.0	0.0	0.0	4.022331987192895E-4	0.0
11	0.0	0.0	0.0	8.04466397438579E-4	0.0
12	0.0	0.0	0.0	8.04466397438579E-4	0.0
13	0.0	0.0	0.0	8.04466397438579E-4	0.0
14	0.0	0.0	0.0	8.04466397438579E-4	0.0
15	0.0	0.0	0.0	8.04466397438579E-4	0.0
16	0.0	0.0	0.0	0.0012066995961578684	0.0
17	0.0	0.0	0.0	0.001608932794877158	0.0
18	0.0	0.0	0.0	0.002413399192315737	0.0
19	0.0	0.0	0.0	0.0028156323910350266	0.0
20	0.0	0.0	0.0	0.0028156323910350266	0.0
21	0.0	0.0	0.0	0.006837964378227921	0.0
22	0.0	0.0	0.0	0.012469229160297975	0.0
23	0.0	0.0	0.0	0.027754090711630975	0.0
24	0.0	0.0	0.0	0.0534970154296655	0.0
25	0.0	0.0	0.0	0.0724019757694721	0.0
26	0.0	0.0	0.0	0.09372033530159445	0.0
27	0.0	0.0	0.0	0.11584316123115537	0.0
28	0.0	0.0	0.0	0.16411114507747013	0.0
29	0.0	0.0	0.0	0.2296751564687143	0.0
30	0.0	0.0	0.0	0.345920550898589	0.0
31	0.0	0.0	0.0	0.522500925136357	0.0
32	0.0	0.0	0.0	0.7300532556755104	0.0
33	0.0	0.0	0.0	0.9038179975222435	0.0
34	0.0	0.0	0.0	1.1182082924396248	0.0
35	0.0	0.0	0.0	1.3675928756455842	0.0
36	0.0	0.0	0.0	1.6724856402748056	0.0
37	0.0	0.0	0.0	2.1052885620967614	0.0
38	0.0	0.0	0.0	2.596817530931733	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACACA	25	0.0023493164	35.199677	5
CCATACT	35	3.2107433E-4	31.428284	2
TTTGCAA	45	4.0569583E-5	29.333067	42
CCGGACG	30	0.0057366085	29.333063	27
AAGACCC	30	0.0057366085	29.333063	5
GCACCGT	30	0.0057366085	29.333063	6
TCGGTCG	40	7.0167007E-4	27.499746	9
ATACATG	100	6.002665E-11	26.410381	1
GACCAGA	95	9.531504E-10	25.47345	11
AGCGGCG	80	1.301214E-7	24.749773	31
GTACAGA	45	0.0013919019	24.45406	1
ACCATAC	45	0.0013919019	24.45406	1
CATACTC	45	0.0013951696	24.444221	3
CGGTCGA	45	0.0013951696	24.444221	10
AGTGTGT	45	0.0013951696	24.444221	8
ACCGTCG	55	1.5887573E-4	23.99978	8
CGGTCCA	55	1.5887573E-4	23.99978	10
TGGTATA	175	0.0	23.885496	44
GCGCAAT	75	2.0534717E-6	23.466454	35
CAGAGCG	95	2.66391E-8	23.157682	14
>>END_MODULE