FastQCFastQC Report
Thu 2 Feb 2017
SRR4062691_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062691_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763320
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24260.1375813805775469No Hit
GCGCAAGACGGACCAGAGCGAAAGC20110.11404623097339112No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18770.10644692965542273No Hit
GTACATGGAAGCAGTGGTATCAACG18500.10491572715105597No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG18450.10463217113172879No Hit
GAATAGGACCGCGGTTCTATTTTGT17670.1002086972302248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC601.4655525E-615.83491814
ACGCTTA508.6970074E-515.20368117
GGGTTAG551.9738708E-413.8070191
CTCGACG655.4481694E-513.15516413
CGGTCCA3550.012.84672110
CGTCGTA2450.012.79756410
ATACCGT2500.012.5408996
TACCGTC2450.012.4094067
GTCGTAG2650.012.19024611
CCGCGCA1103.8078724E-812.0924649
ACCGTCG2600.012.05898
AGAACCG2100.011.7614135
AACCGCG1701.8189894E-1211.7364687
CGCTTAC658.009975E-411.69480818
ACTCTAA2600.011.69381110
CCGTCGT2700.011.6126049
GCCGGTC3850.011.5985648
TTAACCA2950.011.5920774
TCTATAC1157.1124305E-811.5607943
GCTCGTA1406.7848305E-1011.5371979
Produced by FastQC (version 0.11.5)