##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062691_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1763320
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22481058457909	32.0	32.0	32.0	32.0	32.0
2	30.988365129414966	32.0	32.0	32.0	32.0	32.0
3	30.986786289499353	32.0	32.0	32.0	32.0	32.0
4	30.978681124242904	32.0	32.0	32.0	32.0	32.0
5	30.917573667853823	32.0	32.0	32.0	32.0	32.0
6	34.44494986729578	36.0	36.0	36.0	32.0	36.0
7	34.38368758932015	36.0	36.0	36.0	32.0	36.0
8	34.388483088719006	36.0	36.0	36.0	32.0	36.0
9	34.49032336728444	36.0	36.0	36.0	32.0	36.0
10	34.26962264364948	36.0	36.0	36.0	32.0	36.0
11	34.47056745230588	36.0	36.0	36.0	32.0	36.0
12	34.32548828346528	36.0	36.0	36.0	32.0	36.0
13	34.414419957806864	36.0	36.0	36.0	32.0	36.0
14	34.33389004831795	36.0	36.0	36.0	32.0	36.0
15	34.285689494816594	36.0	36.0	36.0	32.0	36.0
16	34.281407798924754	36.0	36.0	36.0	32.0	36.0
17	34.21969523399043	36.0	36.0	36.0	32.0	36.0
18	34.222212644330014	36.0	36.0	36.0	32.0	36.0
19	34.214057006102124	36.0	36.0	36.0	32.0	36.0
20	34.196996574643286	36.0	36.0	36.0	32.0	36.0
21	34.167931515550215	36.0	36.0	36.0	32.0	36.0
22	34.12579849375042	36.0	36.0	36.0	32.0	36.0
23	34.100390173082594	36.0	36.0	36.0	32.0	36.0
24	34.077303609101016	36.0	36.0	36.0	32.0	36.0
25	33.53284259238255	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	9.0
4	73.0
5	178.0
6	282.0
7	73.0
8	262.0
9	336.0
10	226.0
11	73.0
12	136.0
13	108.0
14	301.0
15	423.0
16	757.0
17	1000.0
18	1359.0
19	1754.0
20	2346.0
21	3167.0
22	4267.0
23	5886.0
24	8121.0
25	11456.0
26	15956.0
27	21336.0
28	29503.0
29	40352.0
30	55565.0
31	79989.0
32	122217.0
33	186669.0
34	445348.0
35	723791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.70500215803387	17.135526228477087	10.592798575754362	26.566673037734684
2	17.532985682810015	19.942209480707664	35.78809764839658	26.736707188085738
3	19.308430243274437	23.455977223682606	28.144631884008653	29.090960649034304
4	12.956972688697894	14.967757451179581	35.55523036023512	36.52003949988741
5	14.91976270615286	36.125588511773636	33.52958429326679	15.425064488806717
6	34.55267877558222	35.035685045642474	16.613532005335593	13.798104173439704
7	30.45498784145369	30.16380697279153	20.741486889483497	18.639718296271283
8	28.588862683661276	31.978702812852106	19.574340068171434	19.858094435315188
9	27.540581581823087	13.993386600059607	18.368335154620155	40.09769666349715
10	15.893316647853863	26.21677572035621	31.614252003733796	26.275655628056132
11	37.873755096015174	21.030275156429212	21.76126233547962	19.334707412075993
12	24.463516144914387	23.75089227050973	28.37775141584945	23.407840168726437
13	29.443648572455484	19.112965180553164	25.076951641833755	26.36643460515759
14	23.59961115609516	19.322214638655673	24.69817468221891	32.37999952303026
15	25.44568312340138	26.511268761160178	22.157742784387537	25.885305331050905
16	26.30924533872323	25.513762405468626	23.095065064837737	25.08192719097041
17	24.402409158299328	25.43329133337838	24.8810269414853	25.283272566836995
18	25.393543624542176	24.50538031288152	25.564066891911075	24.53700917066523
19	25.77281067025855	25.017844903512593	24.926874225940043	24.28247020028881
20	25.714611796957243	24.15490646537578	24.68977938479394	25.44070235287304
21	26.825057822292912	24.229769579653368	23.91863936322508	25.02653323482864
22	25.985038624566712	24.19895414635476	24.44130548252452	25.374701746554006
23	24.65042927546207	24.006601267325202	25.162561318981368	26.180408138231353
24	24.826184904166734	24.88416586074462	24.727429876263756	25.56221935882489
25	24.947402313105727	24.339796105528137	24.964040501780513	25.748761079585623
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	109.0
2	377.5
3	646.0
4	646.0
5	646.0
6	1734.0
7	2822.0
8	2822.0
9	2822.0
10	3164.5
11	3507.0
12	3507.0
13	3507.0
14	4357.5
15	5208.0
16	5208.0
17	5208.0
18	8320.5
19	11433.0
20	11433.0
21	11433.0
22	18118.0
23	24803.0
24	24803.0
25	24803.0
26	36097.0
27	47391.0
28	47391.0
29	47391.0
30	57851.5
31	68312.0
32	68312.0
33	68312.0
34	86726.0
35	105140.0
36	105140.0
37	105140.0
38	120601.5
39	136063.0
40	136063.0
41	136063.0
42	156744.5
43	177426.0
44	177426.0
45	177426.0
46	203393.5
47	229361.0
48	229361.0
49	229361.0
50	231420.0
51	233479.0
52	233479.0
53	233479.0
54	220254.5
55	207030.0
56	207030.0
57	207030.0
58	190073.5
59	173117.0
60	173117.0
61	173117.0
62	155108.5
63	137100.0
64	137100.0
65	137100.0
66	115142.0
67	93184.0
68	93184.0
69	93184.0
70	71210.5
71	49237.0
72	49237.0
73	49237.0
74	38913.0
75	28589.0
76	28589.0
77	28589.0
78	22861.5
79	17134.0
80	17134.0
81	17134.0
82	12198.5
83	7263.0
84	7263.0
85	7263.0
86	5195.5
87	3128.0
88	3128.0
89	3128.0
90	2042.5
91	957.0
92	957.0
93	957.0
94	556.0
95	155.0
96	155.0
97	155.0
98	440.5
99	726.0
100	726.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007996279745026428
2	0.003119116212598961
3	0.004820452328562031
4	0.015709003470725676
5	0.03465054556178119
6	0.04633305355806093
7	0.06720277658054125
8	0.08988725812671551
9	0.09896105074518521
10	0.11954721774833836
11	0.12136197627203231
12	0.13400857473402444
13	0.12187237710682122
14	0.12487807091168932
15	0.11540729986616155
16	0.11342240773087131
17	0.1251616269310165
18	0.12873443277453894
19	0.13117301454075267
20	0.1221559331261484
21	0.13162670417167616
22	0.1504548238550008
23	0.1387156046548556
24	0.13593675566544927
25	0.1310595921330218
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1763320.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.07736255895619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3061844385868	43.9682401417082
2	11.688918817101612	12.642118015892184
3	3.0417137257290916	4.934635678404166
4	1.2199852438199055	2.6389433738650236
5	0.6495645317196344	1.7563368343620631
6	0.4089415033688738	1.3268686765849917
7	0.2854141623629695	1.0804111596294166
8	0.20013228323830182	0.8658100832343487
9	0.15462142593466877	0.7525367018686687
>10	0.8360492789804407	8.422275725418029
>50	0.099215803082638	3.761027512241978
>100	0.0932906516511711	10.608919586910114
>500	0.012396314881661406	4.476925501950642
>1k	0.0035718195421736254	2.764951007930179
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2426	0.1375813805775469	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2011	0.11404623097339112	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1877	0.10644692965542273	No Hit
GTACATGGAAGCAGTGGTATCAACG	1850	0.10491572715105597	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1845	0.10463217113172879	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1767	0.1002086972302248	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.1342240773087131E-4	0.0
8	0.0	0.0	0.0	1.1342240773087131E-4	0.0
9	0.0	0.0	0.0	2.2684481546174262E-4	0.0
10	0.0	0.0	0.0	2.2684481546174262E-4	0.0
11	0.0	0.0	0.0	2.2684481546174262E-4	0.0
12	0.0	0.0	0.0	2.2684481546174262E-4	5.6711203865435654E-5
13	0.0	0.0	0.0	2.2684481546174262E-4	1.1342240773087131E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGC	60	1.4655525E-6	15.834918	14
ACGCTTA	50	8.6970074E-5	15.203681	17
GGGTTAG	55	1.9738708E-4	13.807019	1
CTCGACG	65	5.4481694E-5	13.155164	13
CGGTCCA	355	0.0	12.846721	10
CGTCGTA	245	0.0	12.797564	10
ATACCGT	250	0.0	12.540899	6
TACCGTC	245	0.0	12.409406	7
GTCGTAG	265	0.0	12.190246	11
CCGCGCA	110	3.8078724E-8	12.092464	9
ACCGTCG	260	0.0	12.0589	8
AGAACCG	210	0.0	11.761413	5
AACCGCG	170	1.8189894E-12	11.736468	7
CGCTTAC	65	8.009975E-4	11.694808	18
ACTCTAA	260	0.0	11.693811	10
CCGTCGT	270	0.0	11.612604	9
GCCGGTC	385	0.0	11.598564	8
TTAACCA	295	0.0	11.592077	4
TCTATAC	115	7.1124305E-8	11.560794	3
GCTCGTA	140	6.7848305E-10	11.537197	9
>>END_MODULE