FastQCFastQC Report
Thu 2 Feb 2017
SRR4062691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763320
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20570.11665494635120113No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18590.10542612798584489No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18040.10230701177324593No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG17690.10032211963795568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA550.0044845319.999919
CGTCGTA4700.018.7233210
TCTAGCG6200.018.45152928
CATATAG2050.018.2443372
TACCGTC4950.018.222147
CGCAATA6450.018.07743836
ATACCGT5250.018.0189676
ACCGTCG5100.017.6861938
CCGTCGT5000.017.599929
ATATAGC1751.9099389E-1017.599923
TAGCGGC6400.017.5311730
CTAGCGG6400.017.5311729
GTCGTAG5000.017.15992411
GTATCAA26750.016.6158371
ATACGAA6900.016.26079640
TTACACT2850.016.2104534
TATAGGG2051.05501385E-1016.0974884
GCAATAC7200.015.88881737
CGGTCCA7200.015.88881710
ATTCGTG2500.015.83992944
Produced by FastQC (version 0.11.5)