##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062691_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1763320
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255544087289884	32.0	32.0	32.0	32.0	32.0
2	31.25871537781004	32.0	32.0	32.0	32.0	32.0
3	31.394316403148608	32.0	32.0	32.0	32.0	32.0
4	31.53726436494794	32.0	32.0	32.0	32.0	32.0
5	31.427168636435812	32.0	32.0	32.0	32.0	32.0
6	35.02957035591952	36.0	36.0	36.0	36.0	36.0
7	35.045871991470634	36.0	36.0	36.0	36.0	36.0
8	34.98230383594583	36.0	36.0	36.0	36.0	36.0
9	35.146137399904724	36.0	36.0	36.0	36.0	36.0
10	34.9809580790781	36.0	36.0	36.0	36.0	36.0
11	35.150740081210444	36.0	36.0	36.0	36.0	36.0
12	35.037498015107865	36.0	36.0	36.0	36.0	36.0
13	35.10544370845904	36.0	36.0	36.0	36.0	36.0
14	35.06328573372956	36.0	36.0	36.0	36.0	36.0
15	35.00095104688882	36.0	36.0	36.0	36.0	36.0
16	35.02190073270875	36.0	36.0	36.0	36.0	36.0
17	34.98632069051562	36.0	36.0	36.0	36.0	36.0
18	35.01191842660436	36.0	36.0	36.0	36.0	36.0
19	34.99327291699748	36.0	36.0	36.0	36.0	36.0
20	34.989180636526555	36.0	36.0	36.0	36.0	36.0
21	34.988396887689134	36.0	36.0	36.0	36.0	36.0
22	34.968275185445634	36.0	36.0	36.0	32.0	36.0
23	34.92220924165778	36.0	36.0	36.0	32.0	36.0
24	34.8958787968151	36.0	36.0	36.0	32.0	36.0
25	34.87063663997459	36.0	36.0	36.0	32.0	36.0
26	34.82262493478211	36.0	36.0	36.0	32.0	36.0
27	34.8069204682077	36.0	36.0	36.0	32.0	36.0
28	34.79258671143071	36.0	36.0	36.0	32.0	36.0
29	34.76792471020575	36.0	36.0	36.0	32.0	36.0
30	34.75781820656489	36.0	36.0	36.0	32.0	36.0
31	34.75800592064969	36.0	36.0	36.0	32.0	36.0
32	34.72249506612526	36.0	36.0	36.0	32.0	36.0
33	34.70744618106753	36.0	36.0	36.0	32.0	36.0
34	34.69491243790123	36.0	36.0	36.0	32.0	36.0
35	34.670557244289185	36.0	36.0	36.0	32.0	36.0
36	34.634985141664586	36.0	36.0	36.0	32.0	36.0
37	34.639693872921534	36.0	36.0	36.0	32.0	36.0
38	34.59769582378695	36.0	36.0	36.0	32.0	36.0
39	34.597322664065516	36.0	36.0	36.0	32.0	36.0
40	34.58506397023796	36.0	36.0	36.0	32.0	36.0
41	34.55084329560148	36.0	36.0	36.0	32.0	36.0
42	34.52861987614273	36.0	36.0	36.0	32.0	36.0
43	34.51827631966972	36.0	36.0	36.0	32.0	36.0
44	34.492269695801106	36.0	36.0	36.0	32.0	36.0
45	34.45052401152372	36.0	36.0	36.0	32.0	36.0
46	34.470489757956585	36.0	36.0	36.0	32.0	36.0
47	34.44330354104757	36.0	36.0	36.0	32.0	36.0
48	34.40926774493569	36.0	36.0	36.0	32.0	36.0
49	34.395382573781276	36.0	36.0	36.0	32.0	36.0
50	33.837518431141255	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	39.0
22	108.0
23	373.0
24	1005.0
25	2404.0
26	5075.0
27	9663.0
28	17005.0
29	27014.0
30	40635.0
31	59896.0
32	93113.0
33	158968.0
34	350447.0
35	997570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.11750192420149	17.7843862038298	11.15753268873765	26.940579183231062
2	16.90770926072016	20.41085658641492	35.39580193994815	27.285632212916767
3	18.77003327820992	23.740384593572564	28.533059304174635	28.956522824042885
4	12.798372159757912	15.440776989743199	35.8208218823831	35.94002896811579
5	14.79351450672595	36.18424335911803	33.36461901413243	15.657623120023592
6	34.7749748484955	35.287872721477854	16.319952090211952	13.617200339814689
7	30.46656307420094	30.28366944173491	20.595921330217998	18.653846153846153
8	28.607569811491956	32.10738833563959	19.34260372479187	19.942438128076585
9	27.650965224689788	13.748837420320758	18.19681056189461	40.40338679309484
10	15.779438785926548	26.307136537894426	31.644738334505362	26.268686341673664
11	38.187680058072274	20.84652814009936	21.73003198511898	19.23575981670939
12	24.53406074904158	23.587210489304265	28.313805775469003	23.56492298618515
13	29.663475716262504	18.998933829367328	25.00300569380487	26.3345847605653
14	23.68447020393349	19.185853957307806	24.571546854796633	32.55812898396207
15	25.57941836989316	26.48316811469274	22.004457500623822	25.932956014790282
16	26.470918494657802	25.474162375518905	22.916203525168434	25.138715604654855
17	24.537860399700566	25.3071478801352	24.825726470521516	25.329265249642717
18	25.595240795771613	24.41763264750584	25.477338202935375	24.509788353787172
19	25.931821790712974	24.896955742576505	24.862418619422453	24.308803847288072
20	25.800195086541294	24.022015289340562	24.67011092711476	25.507678697003378
21	27.04319125286392	24.030975659551302	23.942846448744415	24.98298663884037
22	26.12526370709797	24.093017716580086	24.424041013542634	25.3576775627793
23	24.794592019599392	23.888914093868387	25.005160719551757	26.311333166980468
24	24.980548057074156	24.90534900074859	24.62701041217703	25.487092530000226
25	25.27947281264887	24.16594832475104	24.787729963931675	25.76684889866842
26	25.00090737926185	24.73924188462673	25.09561508971713	25.1642356463943
27	25.903806456003448	24.455118753260894	24.267234534854705	25.373840255880953
28	24.91975364653041	24.203888120137016	25.279869791075924	25.596488442256653
29	25.00873352539528	24.620205067713176	24.94408275298868	25.426978653902864
30	25.019281809314247	24.69052696050632	25.209150919855727	25.08104031032371
31	25.32228977156727	24.60750175804732	24.33239570809609	25.73781276228932
32	25.253896059705554	24.52595104688882	24.36347344781435	25.85667944559127
33	24.964668919991833	24.05383027470907	24.893156091917522	26.088344713381577
34	25.33907628791144	24.36710296486174	25.189528843318286	25.10429190390854
35	26.22978245582197	24.151089989338292	24.7804709298369	24.838656625002837
36	25.000283556019326	24.666935099698296	24.675271646666516	25.65750969761586
37	26.133373409250733	24.408048454052583	24.3811106322165	25.077467504480182
38	24.93637002926298	24.147347049883177	25.16083297416238	25.755449946691467
39	25.380021777102286	24.30897398089967	24.40782160923712	25.90318263276093
40	25.665505977360887	24.262754349749336	25.008790236599143	25.062949436290634
41	24.68576321938162	24.71423224372207	25.27244062336955	25.327563913526756
42	25.89002563346415	24.702776580541254	24.81897783726153	24.588219948733073
43	25.11427307578885	23.711861715400495	25.046843454392846	26.1270217544178
44	24.897352721003564	24.26519293151555	24.973402445387112	25.864051902093777
45	24.890377242928114	24.553852959190618	25.101910033346186	25.453859764535082
46	24.79833495905451	24.24704534627861	25.157543724338183	25.7970759703287
47	24.911587233173783	24.009822380509494	25.599550847265384	25.479039539051335
48	25.50421931356759	24.953099834403282	24.573304902116462	24.969375949912664
49	24.455969421318876	24.730678492843047	25.206995894108843	25.60635619172924
50	24.474967674613797	24.915557017444367	24.98049134587029	25.628983962071544
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	9.0
2	12.0
3	52.0
4	92.0
5	111.5
6	131.0
7	153.0
8	175.0
9	235.0
10	295.0
11	396.5
12	498.0
13	911.0
14	1324.0
15	1815.0
16	2306.0
17	2873.5
18	3441.0
19	3918.5
20	4396.0
21	5377.0
22	6358.0
23	6983.5
24	7609.0
25	9635.0
26	11661.0
27	14462.5
28	17264.0
29	19383.0
30	21502.0
31	23152.5
32	24803.0
33	28148.5
34	31494.0
35	36614.0
36	41734.0
37	51852.5
38	61971.0
39	67805.5
40	73640.0
41	81635.0
42	89630.0
43	91870.5
44	94111.0
45	103805.0
46	113499.0
47	121340.0
48	129181.0
49	132516.5
50	135852.0
51	131130.5
52	126409.0
53	123901.0
54	121393.0
55	123206.5
56	125020.0
57	123867.5
58	122715.0
59	111706.0
60	100697.0
61	89068.0
62	77439.0
63	68394.0
64	59349.0
65	51340.0
66	43331.0
67	39143.0
68	34955.0
69	32038.5
70	29122.0
71	22946.5
72	16771.0
73	14838.0
74	12905.0
75	9725.5
76	6546.0
77	5813.5
78	5081.0
79	4360.5
80	3640.0
81	2862.0
82	2084.0
83	1732.0
84	1380.0
85	1122.5
86	865.0
87	649.0
88	433.0
89	278.0
90	123.0
91	85.5
92	48.0
93	31.5
94	15.0
95	11.5
96	8.0
97	5.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01423451217022435
2	0.004366762697638546
3	2.2684481546174262E-4
4	1.1342240773087131E-4
5	0.0
6	3.402672231926139E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1763320.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.062393724002824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.98201428712414	46.78089977538703
2	11.437527061589305	13.053053448346924
3	2.8829466271015187	4.935235065628585
4	1.1738182671646507	2.6792352048550407
5	0.6296303254310641	1.7964106765159706
6	0.3649596427502692	1.2495282496792364
7	0.26375233022952765	1.0535237519226364
8	0.1860208669204495	0.8491836759276018
9	0.13494222338212	0.6930113652560582
>10	0.7660622125425464	8.1589178384423
>50	0.08560756367204671	3.420820274424072
>100	0.08081673765765451	9.768983348620456
>500	0.009601496014603323	3.6998744022869676
>1k	0.0023003584201653797	1.8613229227072325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2057	0.11665494635120113	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1859	0.10542612798584489	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1804	0.10230701177324593	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1769	0.10032211963795568	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.6711203865435654E-5
7	0.0	0.0	0.0	0.0	5.6711203865435654E-5
8	0.0	0.0	0.0	0.0	5.6711203865435654E-5
9	0.0	0.0	0.0	0.0	1.1342240773087131E-4
10	0.0	0.0	0.0	0.0	1.1342240773087131E-4
11	0.0	0.0	0.0	0.0	1.1342240773087131E-4
12	0.0	0.0	0.0	0.0	2.2684481546174262E-4
13	0.0	0.0	0.0	0.0	2.2684481546174262E-4
14	0.0	0.0	0.0	5.6711203865435654E-5	2.835560193271783E-4
15	0.0	0.0	0.0	5.6711203865435654E-5	3.969784270580496E-4
16	0.0	0.0	0.0	5.6711203865435654E-5	3.969784270580496E-4
17	0.0	0.0	0.0	5.6711203865435654E-5	5.671120386543566E-4
18	0.0	0.0	0.0	5.6711203865435654E-5	6.805344463852278E-4
19	0.0	0.0	0.0	1.1342240773087131E-4	6.805344463852278E-4
20	0.0	0.0	0.0	1.1342240773087131E-4	7.939568541160992E-4
21	0.0	0.0	0.0	1.7013361159630696E-4	7.939568541160992E-4
22	0.0	0.0	0.0	2.835560193271783E-4	7.939568541160992E-4
23	0.0	0.0	0.0	2.835560193271783E-4	8.506680579815348E-4
24	0.0	0.0	0.0	7.372456502506635E-4	8.506680579815348E-4
25	0.0	0.0	0.0	0.0012476464850395845	8.506680579815348E-4
26	0.0	0.0	0.0	0.0017580473198285053	8.506680579815348E-4
27	0.0	0.0	0.0	0.003175827416464397	8.506680579815348E-4
28	0.0	0.0	0.0	0.007656012521833813	9.073792618469705E-4
29	0.0	0.0	0.0	0.018828119683324637	9.073792618469705E-4
30	0.0	0.0	0.0	0.04043508835605562	9.640904657124062E-4
31	0.0	0.0	0.0	0.0879590771952907	9.640904657124062E-4
32	0.0	0.0	0.0	0.14410316902207201	9.640904657124062E-4
33	0.0	0.0	0.0	0.20257242020733615	9.640904657124062E-4
34	0.0	0.0	0.0	0.27011546401107	0.0010208016695778417
35	0.0	0.0	0.0	0.3469024340448699	0.0010208016695778417
36	0.0	0.0	0.0	0.4562416350974298	0.0010775128734432774
37	0.0	0.0	0.0	0.6171880316675362	0.0010775128734432774
38	0.0	0.0	0.0	0.8301953133861125	0.0011342240773087131
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	55	0.00448453	19.99991	9
CGTCGTA	470	0.0	18.72332	10
TCTAGCG	620	0.0	18.451529	28
CATATAG	205	0.0	18.244337	2
TACCGTC	495	0.0	18.22214	7
CGCAATA	645	0.0	18.077438	36
ATACCGT	525	0.0	18.018967	6
ACCGTCG	510	0.0	17.686193	8
CCGTCGT	500	0.0	17.59992	9
ATATAGC	175	1.9099389E-10	17.59992	3
TAGCGGC	640	0.0	17.53117	30
CTAGCGG	640	0.0	17.53117	29
GTCGTAG	500	0.0	17.159924	11
GTATCAA	2675	0.0	16.615837	1
ATACGAA	690	0.0	16.260796	40
TTACACT	285	0.0	16.210453	4
TATAGGG	205	1.05501385E-10	16.097488	4
GCAATAC	720	0.0	15.888817	37
CGGTCCA	720	0.0	15.888817	10
ATTCGTG	250	0.0	15.839929	44
>>END_MODULE