FastQCFastQC Report
Thu 2 Feb 2017
SRR4062690_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062690_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078455
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29760.27595031781576423No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19950.18498685619705968No Hit
TATCAACGCAGAGTACTTTTTTTTT18340.17005809236361275No Hit
GTACATGGAAGCAGTGGTATCAACG16200.15021489074648456No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14810.13732608221947137No Hit
TCCATGTACTCTGCGTTGATACCAC13470.12490089989846587No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12410.11507202433110328No Hit
GCGCAAGACGGACCAGAGCGAAAGC11050.1024613915276947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTGCG951.8189894E-1116.00203316
GTTCTAG551.1342652E-515.5358881
TCCAACG807.687049E-915.44017918
CGGTCTG456.760573E-414.77759715
TTTGCGC1101.2732926E-1114.68368317
TTGCGCG1006.566552E-1014.25247218
ATCCCGT602.5648656E-514.25114910
CGTTATT903.6552592E-813.7163232
CGACCAT1351.8189894E-1213.371448510
CAAGACG1550.012.8714084
TCGCGTA604.0874328E-412.6676889
TTAGACC604.089034E-412.66714
AATTTGC1301.9826984E-1012.42292515
TGGATTG1001.4410398E-712.349855
CTAAACG550.003066608412.09132312
AACGAGC550.003066608412.09132312
GCGCAAG1650.012.0834681
GTGCTAT951.0465301E-611.9926161
CGGTCCA1750.011.94382110
TTGACGG802.8634444E-511.87540612
Produced by FastQC (version 0.11.5)