##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062690_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1078455
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11051550597846	32.0	32.0	32.0	32.0	32.0
2	30.8650022485871	32.0	32.0	32.0	32.0	32.0
3	30.853931782040046	32.0	32.0	32.0	32.0	32.0
4	30.88620016597818	32.0	32.0	32.0	32.0	32.0
5	30.77088427426272	32.0	32.0	32.0	32.0	32.0
6	34.379630119012845	36.0	36.0	36.0	32.0	36.0
7	34.25621838648808	36.0	36.0	36.0	32.0	36.0
8	34.25654941559917	36.0	36.0	36.0	32.0	36.0
9	34.35648589880894	36.0	36.0	36.0	32.0	36.0
10	34.1169932913288	36.0	36.0	36.0	32.0	36.0
11	34.36794210235939	36.0	36.0	36.0	32.0	36.0
12	34.18409854838635	36.0	36.0	36.0	32.0	36.0
13	34.27917622895716	36.0	36.0	36.0	32.0	36.0
14	34.20771010380591	36.0	36.0	36.0	32.0	36.0
15	34.17523772433713	36.0	36.0	36.0	32.0	36.0
16	34.164346217505596	36.0	36.0	36.0	32.0	36.0
17	34.093842580358015	36.0	36.0	36.0	32.0	36.0
18	34.112364447288016	36.0	36.0	36.0	32.0	36.0
19	34.107693876888696	36.0	36.0	36.0	32.0	36.0
20	34.0819570589408	36.0	36.0	36.0	32.0	36.0
21	34.0491434505844	36.0	36.0	36.0	32.0	36.0
22	34.012605996541346	36.0	36.0	36.0	32.0	36.0
23	33.993274638255656	36.0	36.0	36.0	32.0	36.0
24	33.96835102067309	36.0	36.0	36.0	32.0	36.0
25	33.51353278532716	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	51.0
5	112.0
6	173.0
7	50.0
8	154.0
9	186.0
10	127.0
11	36.0
12	90.0
13	69.0
14	250.0
15	302.0
16	480.0
17	672.0
18	919.0
19	1178.0
20	1753.0
21	2443.0
22	3340.0
23	4744.0
24	6609.0
25	8955.0
26	12361.0
27	15918.0
28	21268.0
29	28389.0
30	36207.0
31	49421.0
32	71536.0
33	102394.0
34	234495.0
35	473768.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.34149625546657	17.749533556138328	10.9367639367491	25.972206251646
2	16.588466132059377	20.514936611203012	37.155423098892456	25.741174157845155
3	19.386552447753473	24.138420266376855	28.404196228290697	28.07083105757898
4	12.614591902125879	15.486843435350458	36.33934690614616	35.5592177563775
5	14.289861928547909	36.74871181236753	33.87977533822173	15.081650920862844
6	33.447902867299725	35.735026179370436	17.175782134203136	13.641288819126713
7	29.51577943310582	30.81378301841274	21.11832997442778	18.55210757405366
8	27.618610090210495	33.103909121282996	20.042785016891266	19.23469577161525
9	27.244308768399566	14.38737408379223	19.129610739899555	39.238706407908644
10	15.582658971343058	27.11821766056345	32.372624724863094	24.926498643230403
11	36.86863623198093	21.52754569287555	22.595414745003676	19.008403330139846
12	24.2218712600008	24.20023787439452	29.30618111321874	22.27170975238594
13	29.30500019960321	20.053809311164688	25.557101835699637	25.084088653532465
14	22.880981426499776	20.303010047106838	25.7233601268561	31.092648399537286
15	24.572696385139526	27.86366758879338	23.019346094576782	24.54428993149032
16	24.867000414941998	26.360234632525696	24.824299448879053	23.948465503653253
17	23.274463875728905	26.3145825616059	26.037178462375444	24.373775100289752
18	23.841338753015144	25.338838432699323	27.57408580007465	23.24573701421088
19	24.800888365641196	25.69519625192891	26.125544783173048	23.378370599256847
20	24.784333094425527	25.07520025549519	26.24024717674103	23.90021947333826
21	25.630007891927022	24.956594401374126	25.11656840443805	24.296829302260807
22	24.85942443651371	25.54152353729443	25.574862349708354	24.024189676483505
23	23.560545844878433	25.2089260643158	26.256992058952434	24.97353603185333
24	23.936571252418783	25.76611809973742	26.2338113330882	24.063499314755596
25	24.08250769006508	25.426377317813003	26.169154506011388	24.32196048611053
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	230.0
1	230.0
2	503.5
3	777.0
4	777.0
5	777.0
6	2045.5
7	3314.0
8	3314.0
9	3314.0
10	3509.0
11	3704.0
12	3704.0
13	3704.0
14	4002.0
15	4300.0
16	4300.0
17	4300.0
18	6795.5
19	9291.0
20	9291.0
21	9291.0
22	14361.0
23	19431.0
24	19431.0
25	19431.0
26	28475.0
27	37519.0
28	37519.0
29	37519.0
30	46555.5
31	55592.0
32	55592.0
33	55592.0
34	65078.0
35	74564.0
36	74564.0
37	74564.0
38	82570.5
39	90577.0
40	90577.0
41	90577.0
42	101853.0
43	113129.0
44	113129.0
45	113129.0
46	128204.5
47	143280.0
48	143280.0
49	143280.0
50	141521.0
51	139762.0
52	139762.0
53	139762.0
54	127598.5
55	115435.0
56	115435.0
57	115435.0
58	105409.5
59	95384.0
60	95384.0
61	95384.0
62	84080.0
63	72776.0
64	72776.0
65	72776.0
66	59812.0
67	46848.0
68	46848.0
69	46848.0
70	35637.0
71	24426.0
72	24426.0
73	24426.0
74	19102.0
75	13778.0
76	13778.0
77	13778.0
78	11078.0
79	8378.0
80	8378.0
81	8378.0
82	5854.5
83	3331.0
84	3331.0
85	3331.0
86	2451.0
87	1571.0
88	1571.0
89	1571.0
90	1057.0
91	543.0
92	543.0
93	543.0
94	315.0
95	87.0
96	87.0
97	87.0
98	257.5
99	428.0
100	428.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007696195019727296
2	0.002874482477247544
3	0.005378063989688954
4	0.01594874148666379
5	0.036162844068598134
6	0.047011697289177576
7	0.06704034938870884
8	0.09040711017149533
9	0.09754695374401343
10	0.11720470487873856
11	0.11711197963753704
12	0.13129894154137167
13	0.12221186790362139
14	0.12323184555683825
15	0.11451567288389408
16	0.11089938847703426
17	0.12295366983323365
18	0.1291662609937364
19	0.13176256774737935
20	0.12341729603924131
21	0.1302789638881548
22	0.15132759364090295
23	0.14084964138512965
24	0.13602792884264991
25	0.13129894154137167
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1078455.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.00381573298308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.84648861675406	48.29255132734653
2	11.850696516799983	13.984726271695413
3	2.9399906306484413	5.204119962824321
4	1.1044221155282141	2.606604759842324
5	0.5648231692944446	1.6663361101384462
6	0.33682418477064735	1.1924347279571714
7	0.21765737284563028	0.8989830864216135
8	0.15736743444568504	0.7428223283524394
9	0.10988273238937002	0.5835150444725172
>10	0.6953244745634279	8.01298382729542
>50	0.08167841412534764	3.417173807856777
>100	0.08809217589962157	9.940441807525978
>500	0.005495945761253014	2.197333280129597
>1k	0.001256216174000689	1.25997365814144
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2976	0.27595031781576423	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1995	0.18498685619705968	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1834	0.17005809236361275	No Hit
GTACATGGAAGCAGTGGTATCAACG	1620	0.15021489074648456	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1481	0.13732608221947137	No Hit
TCCATGTACTCTGCGTTGATACCAC	1347	0.12490089989846587	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1241	0.11507202433110328	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1105	0.1024613915276947	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	1.8545048240306734E-4	0.0
7	0.0	0.0	0.0	2.78175723604601E-4	0.0
8	0.0	0.0	0.0	2.78175723604601E-4	9.272524120153367E-5
9	0.0	0.0	0.0	2.78175723604601E-4	1.8545048240306734E-4
10	0.0	0.0	0.0	2.78175723604601E-4	1.8545048240306734E-4
11	0.0	0.0	0.0	2.78175723604601E-4	1.8545048240306734E-4
12	0.0	0.0	0.0	2.78175723604601E-4	1.8545048240306734E-4
13	0.0	0.0	0.0	2.78175723604601E-4	1.8545048240306734E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTGCG	95	1.8189894E-11	16.002033	16
GTTCTAG	55	1.1342652E-5	15.535888	1
TCCAACG	80	7.687049E-9	15.440179	18
CGGTCTG	45	6.760573E-4	14.777597	15
TTTGCGC	110	1.2732926E-11	14.683683	17
TTGCGCG	100	6.566552E-10	14.252472	18
ATCCCGT	60	2.5648656E-5	14.251149	10
CGTTATT	90	3.6552592E-8	13.716323	2
CGACCAT	135	1.8189894E-12	13.3714485	10
CAAGACG	155	0.0	12.871408	4
TCGCGTA	60	4.0874328E-4	12.667688	9
TTAGACC	60	4.089034E-4	12.6671	4
AATTTGC	130	1.9826984E-10	12.422925	15
TGGATTG	100	1.4410398E-7	12.34985	5
CTAAACG	55	0.0030666084	12.091323	12
AACGAGC	55	0.0030666084	12.091323	12
GCGCAAG	165	0.0	12.083468	1
GTGCTAT	95	1.0465301E-6	11.992616	1
CGGTCCA	175	0.0	11.943821	10
TTGACGG	80	2.8634444E-5	11.875406	12
>>END_MODULE