##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062690_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1078455
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.257918040159304	32.0	32.0	32.0	32.0	32.0
2	31.396867741352214	32.0	32.0	32.0	32.0	32.0
3	31.479050122629133	32.0	32.0	32.0	32.0	32.0
4	31.577962919176045	32.0	32.0	32.0	32.0	32.0
5	31.518992447529104	32.0	32.0	32.0	32.0	32.0
6	35.09024947726145	36.0	36.0	36.0	36.0	36.0
7	35.10135981566222	36.0	36.0	36.0	36.0	36.0
8	35.05208098622567	36.0	36.0	36.0	36.0	36.0
9	35.156186396279864	36.0	36.0	36.0	36.0	36.0
10	35.04866035207774	36.0	36.0	36.0	36.0	36.0
11	35.178990314848555	36.0	36.0	36.0	36.0	36.0
12	35.08527384081858	36.0	36.0	36.0	36.0	36.0
13	35.12664877069511	36.0	36.0	36.0	36.0	36.0
14	35.09040432841426	36.0	36.0	36.0	36.0	36.0
15	35.06087319359639	36.0	36.0	36.0	36.0	36.0
16	35.06997695777756	36.0	36.0	36.0	36.0	36.0
17	35.04379134966225	36.0	36.0	36.0	36.0	36.0
18	35.054182140191294	36.0	36.0	36.0	36.0	36.0
19	35.03504735941694	36.0	36.0	36.0	36.0	36.0
20	35.030373080007976	36.0	36.0	36.0	36.0	36.0
21	35.01832528941866	36.0	36.0	36.0	36.0	36.0
22	35.00341507063345	36.0	36.0	36.0	36.0	36.0
23	34.96219128290007	36.0	36.0	36.0	32.0	36.0
24	34.94822129806065	36.0	36.0	36.0	32.0	36.0
25	34.924326930655425	36.0	36.0	36.0	32.0	36.0
26	34.88142017979424	36.0	36.0	36.0	32.0	36.0
27	34.86156121488611	36.0	36.0	36.0	32.0	36.0
28	34.84241994334488	36.0	36.0	36.0	32.0	36.0
29	34.817132842816804	36.0	36.0	36.0	32.0	36.0
30	34.811234590223975	36.0	36.0	36.0	32.0	36.0
31	34.797972098974924	36.0	36.0	36.0	32.0	36.0
32	34.76651413364489	36.0	36.0	36.0	32.0	36.0
33	34.73731495519053	36.0	36.0	36.0	32.0	36.0
34	34.71284847304709	36.0	36.0	36.0	32.0	36.0
35	34.68546300031063	36.0	36.0	36.0	32.0	36.0
36	34.65565925328363	36.0	36.0	36.0	32.0	36.0
37	34.63943789958784	36.0	36.0	36.0	32.0	36.0
38	34.59875655451549	36.0	36.0	36.0	32.0	36.0
39	34.57912291194347	36.0	36.0	36.0	32.0	36.0
40	34.56940530666555	36.0	36.0	36.0	32.0	36.0
41	34.54010969396034	36.0	36.0	36.0	32.0	36.0
42	34.51113305608486	36.0	36.0	36.0	32.0	36.0
43	34.502202688104745	36.0	36.0	36.0	32.0	36.0
44	34.45883138378514	36.0	36.0	36.0	32.0	36.0
45	34.41720238674771	36.0	36.0	36.0	32.0	36.0
46	34.406038267707046	36.0	36.0	36.0	32.0	36.0
47	34.37442637847662	36.0	36.0	36.0	32.0	36.0
48	34.32757138684507	36.0	36.0	36.0	32.0	36.0
49	34.31156422845645	36.0	36.0	36.0	32.0	36.0
50	33.776998576667545	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	11.0
21	29.0
22	116.0
23	307.0
24	784.0
25	1679.0
26	3723.0
27	6961.0
28	11761.0
29	18015.0
30	25860.0
31	36922.0
32	53709.0
33	85921.0
34	183912.0
35	648740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93098107590505	18.314127919647948	11.39461444648993	26.360276557957068
2	16.045693026982217	21.041548051119516	36.7484101652983	26.16434875659997
3	18.80237266667223	24.400875702049888	28.690183773177964	28.10656785809992
4	12.31775945314159	15.906795950859149	36.53323145882381	35.24221313717545
5	14.024414556008363	37.017121715787866	33.75254414880547	15.205919579398305
6	33.60418526977168	35.895995081825845	16.888791217758094	13.611028430644378
7	29.516762405478207	30.773838500447397	21.14107681822607	18.56832227584832
8	27.755472185183606	33.26168756386457	19.91999658770796	19.062843663243868
9	27.285144025480896	14.140784733716288	19.01516521319851	39.55890602760431
10	15.521649025689527	27.1888952251137	32.2793255165955	25.01013023260127
11	37.11578137242629	21.368068208687426	22.547626002012137	18.96852441687414
12	24.348072010422317	24.040316934874426	29.37285283113343	22.23875822356983
13	29.548567163210333	19.827531051365145	25.556096452795895	25.067805332628623
14	22.899240116648354	20.258796148193483	25.66810854416735	31.173855190990817
15	24.73890890208678	27.85716603845316	22.92993217148606	24.473992887974
16	24.9879688999541	26.26896810715329	24.734736266232712	24.0083267266599
17	23.27932088033344	26.275180698313793	26.058389084384604	24.387109336968162
18	24.09474665145973	25.143005503243067	27.463547389552645	23.298700455744562
19	24.891349198622102	25.555169200383883	26.063210796927084	23.490270804066927
20	24.945871640448605	24.94614981617221	26.197569671428106	23.910408871951077
21	25.79430759744264	24.796676727355337	25.14801266626795	24.261003008934075
22	24.940864477423723	25.34496107858001	25.729585379083968	23.984589064912303
23	23.768724703395137	24.99649962214464	26.23716334942116	24.99761232503906
24	24.109768140534378	25.602459073396666	26.17531561353974	24.11245717252922
25	24.253492264396755	25.150238072056787	26.209716678025508	24.386552985520954
26	23.52235373752266	25.9432243348123	26.636067337070163	23.898354590594877
27	24.43588281384017	25.701675081482307	25.83121224344085	24.031229861236678
28	23.6760921874348	25.408663319285456	26.4509877556319	24.464256737647837
29	23.735436341803783	25.59188839589969	26.2211218826933	24.451553379603226
30	23.833632372236206	25.560639989614774	26.584975729168114	24.020751908980902
31	24.170410448280176	25.419512172506035	25.897788966623548	24.512288412590234
32	23.840123139120315	25.71020580367285	25.909008720808934	24.540662336397904
33	23.57186901632428	25.30583102679296	26.46183660885248	24.660463348030284
34	24.063220069451205	25.523178992169353	26.747615802235607	23.665985136143835
35	24.618551539007193	25.369718717980817	26.357149811535947	23.654579931476047
36	23.52967903157758	25.82110519214988	26.29752748144336	24.351688294829177
37	24.45656054262811	25.548956609223378	25.802374693427172	24.192108154721335
38	23.78040808378653	25.539498634620823	26.26766995377647	24.41242332781618
39	24.164105131878475	25.16498138540783	25.957318571474936	24.71359491123876
40	24.587952209410684	25.23035268045491	26.472500011590654	23.709195098543752
41	23.60144836826757	25.242128786087502	27.011233662971566	24.145189182673363
42	24.484192664506168	25.767788178459	26.370502246268966	23.377516910765863
43	23.577432530796372	25.02348266733429	26.59128104556982	24.80780375629952
44	23.107964634593007	25.540518612274038	26.58766476116296	24.763851991969993
45	23.2588286020279	25.670333949956188	26.62419850619636	24.446638941819547
46	23.230105317426613	25.102322398544587	26.681249269788047	24.986323014240757
47	23.55939479977338	25.012216573184	26.97326633613148	24.455122290911145
48	23.751385083290447	25.92440111084839	25.996170447538375	24.328043358322784
49	23.034711694043793	25.687024493372462	26.55678725584285	24.721476556740893
50	22.778254798072055	26.330932983697032	26.129069946423307	24.761742271807606
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	46.5
2	40.0
3	154.5
4	269.0
5	300.0
6	331.0
7	303.5
8	276.0
9	356.5
10	437.0
11	659.0
12	881.0
13	1457.0
14	2033.0
15	2734.0
16	3435.0
17	3793.0
18	4151.0
19	4484.0
20	4817.0
21	5734.5
22	6652.0
23	7081.0
24	7510.0
25	8769.5
26	10029.0
27	12166.5
28	14304.0
29	16450.5
30	18597.0
31	20538.0
32	22479.0
33	26070.5
34	29662.0
35	33778.5
36	37895.0
37	42929.5
38	47964.0
39	50082.0
40	52200.0
41	55173.0
42	58146.0
43	59067.5
44	59989.0
45	64861.5
46	69734.0
47	73662.0
48	77590.0
49	78495.5
50	79401.0
51	75928.0
52	72455.0
53	70499.5
54	68544.0
55	68537.0
56	68530.0
57	66663.0
58	64796.0
59	59073.5
60	53351.0
61	46527.0
62	39703.0
63	34801.0
64	29899.0
65	25493.5
66	21088.0
67	18665.0
68	16242.0
69	14654.0
70	13066.0
71	10216.5
72	7367.0
73	6530.0
74	5693.0
75	4258.5
76	2824.0
77	2484.5
78	2145.0
79	1875.5
80	1606.0
81	1292.0
82	978.0
83	800.5
84	623.0
85	504.5
86	386.0
87	287.5
88	189.0
89	122.0
90	55.0
91	39.0
92	23.0
93	17.0
94	11.0
95	7.0
96	3.0
97	2.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018545048240306734
2	0.003801734889262881
3	5.56351447209202E-4
4	3.709009648061347E-4
5	0.0
6	5.56351447209202E-4
7	0.0
8	9.272524120153367E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.272524120153367E-5
47	1.8545048240306734E-4
48	0.0
49	0.0
50	9.272524120153367E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1078455.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.48259732894882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.89895145241422	52.626407540456896
2	11.401017484479965	14.475324042150934
3	2.7210122379973347	5.1821077269578
4	1.0306214470669883	2.6170610529092873
5	0.5038430885075149	1.5992633952348247
6	0.28608277103342916	1.0896766413759031
7	0.19132003446117826	0.8501844896061714
8	0.1350549323606467	0.6858910310671484
9	0.10383826383409209	0.5932730421285136
>10	0.5839397771201807	7.14365520640526
>50	0.07401159927917526	3.302785399322178
>100	0.06634389256321717	8.07385038183299
>500	0.003963018882086431	1.7605200505520446
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8545048240306734E-4
2	0.0	0.0	0.0	0.0	1.8545048240306734E-4
3	0.0	0.0	0.0	0.0	1.8545048240306734E-4
4	0.0	0.0	0.0	0.0	1.8545048240306734E-4
5	0.0	0.0	0.0	0.0	2.78175723604601E-4
6	0.0	0.0	0.0	0.0	4.6362620600766836E-4
7	0.0	0.0	0.0	9.272524120153367E-5	4.6362620600766836E-4
8	0.0	0.0	0.0	9.272524120153367E-5	4.6362620600766836E-4
9	0.0	0.0	0.0	1.8545048240306734E-4	4.6362620600766836E-4
10	0.0	0.0	0.0	1.8545048240306734E-4	4.6362620600766836E-4
11	0.0	0.0	0.0	1.8545048240306734E-4	4.6362620600766836E-4
12	0.0	0.0	0.0	1.8545048240306734E-4	4.6362620600766836E-4
13	0.0	0.0	0.0	1.8545048240306734E-4	4.6362620600766836E-4
14	0.0	0.0	0.0	1.8545048240306734E-4	5.56351447209202E-4
15	0.0	0.0	0.0	1.8545048240306734E-4	6.490766884107357E-4
16	0.0	0.0	0.0	2.78175723604601E-4	6.490766884107357E-4
17	0.0	0.0	0.0	2.78175723604601E-4	6.490766884107357E-4
18	0.0	0.0	0.0	3.709009648061347E-4	6.490766884107357E-4
19	0.0	0.0	0.0	4.6362620600766836E-4	6.490766884107357E-4
20	0.0	0.0	0.0	7.418019296122694E-4	6.490766884107357E-4
21	9.272524120153367E-5	0.0	0.0	0.0010199776532168704	7.418019296122694E-4
22	9.272524120153367E-5	0.0	0.0	0.001947230065232207	7.418019296122694E-4
23	9.272524120153367E-5	0.0	0.0	0.0029672077184490775	8.34527170813803E-4
24	9.272524120153367E-5	0.0	0.0	0.005285338748487419	8.34527170813803E-4
25	9.272524120153367E-5	0.0	0.0	0.006768942607711958	8.34527170813803E-4
26	9.272524120153367E-5	0.0	0.0	0.008437996949339565	8.34527170813803E-4
27	9.272524120153367E-5	0.0	0.0	0.012239731838602445	8.34527170813803E-4
28	9.272524120153367E-5	0.0	0.0	0.02420128795360029	8.34527170813803E-4
29	9.272524120153367E-5	0.0	0.0	0.04979345452522358	8.34527170813803E-4
30	9.272524120153367E-5	0.0	0.0	0.09420884506075822	8.34527170813803E-4
31	9.272524120153367E-5	0.0	0.0	0.17831063883054926	8.34527170813803E-4
32	9.272524120153367E-5	0.0	0.0	0.2806793051170424	8.34527170813803E-4
33	9.272524120153367E-5	0.0	0.0	0.3835115976095433	8.34527170813803E-4
34	9.272524120153367E-5	0.0	0.0	0.5074852450959938	8.34527170813803E-4
35	9.272524120153367E-5	0.0	0.0	0.6476858097927127	8.34527170813803E-4
36	9.272524120153367E-5	0.0	0.0	0.844077870657561	8.34527170813803E-4
37	1.8545048240306734E-4	0.0	0.0	1.1331024474827416	8.34527170813803E-4
38	1.8545048240306734E-4	0.0	0.0	1.4889819232142278	8.34527170813803E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	55	0.0044834944	19.999853	7
ATACCGT	255	0.0	19.84299	6
TGTAGGA	545	0.0	19.781506	2
TAGGACG	470	0.0	19.65943	4
CGTCGTA	225	0.0	19.555412	10
AGGACGT	455	0.0	19.340517	5
CTGTAGG	560	0.0	19.254322	1
CGCGGGA	195	0.0	19.179346	44
CGCAATA	255	0.0	18.980251	36
GTAGGAC	635	0.0	18.708525	3
GGACGTG	480	0.0	18.333199	6
ACCGTCG	255	0.0	18.117514	8
GACGTGA	280	0.0	18.071295	7
TAGCGTA	220	0.0	17.999866	7
AGCGTAT	225	0.0	17.59987	8
GTCGTAG	250	0.0	17.59987	11
CGAGCCG	300	0.0	17.59987	15
TTAGGAC	365	0.0	17.479322	3
CCGTCGT	240	0.0	17.416538	9
CGTATAT	230	0.0	17.217264	10
>>END_MODULE