FastQCFastQC Report
Thu 2 Feb 2017
SRR4062689_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062689_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2611155
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85280.32659876568032153No Hit
TATCAACGCAGAGTACTTTTTTTTT50440.19317122116458044No Hit
GTACATGGAAGCAGTGGTATCAACG38340.1468315745331089No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34180.13089992742675177No Hit
GGTATCAACGCAGAGTACTTTTTTT33930.12994249671122549No Hit
TCCATGTACTCTGCGTTGATACCAC32960.12622766553498357No Hit
GTCTTGCGCCGGTCCAAGAATTTCA28070.10750032073928971No Hit
GAATAGGACCGCGGTTCTATTTTGT27700.10608332328031081No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG27530.10543227039375294No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27240.10432165076374247No Hit
GCGCAAGACGGACCAGAGCGAAAGC26650.10206211427510048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA2100.013.12098115
ACTATAC1750.011.9388933
AATCGTA1201.0017175E-811.87561113
GATAGTC1251.832268E-811.4005877
TATACCG752.077548E-411.39845
CGGTCCA5550.011.29744510
GTATTAG4750.011.1932781
TCACGTA855.317849E-511.17811815
TACCGTA600.005875217311.0839047
GTATACG600.005903790211.0766811
GTGCTAA1900.010.9933991
TCGCGTA2000.010.9253539
GATCACG700.001491349410.85770113
GCCGGTC5800.010.8109018
CGCCGGT5450.010.8078967
CGCATCG3650.010.671782513
AACCGCG2500.010.6405487
CAAGACG4750.010.5979044
TCGGCGT2600.010.59669913
GTATAGG1803.6379788E-1110.5492211
Produced by FastQC (version 0.11.5)