FastQCFastQC Report
Thu 2 Feb 2017
SRR4062689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2611155
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC29430.11270874383175261No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT27020.10347911173407938No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA26610.10190892536061627No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26170.10022384730129005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA3600.021.38865715
CGCAATA8150.020.24517836
ATACGAA8350.019.76026540
TAGCGGC8650.019.3292730
CAATACG8750.019.10836438
CTAGCGG8650.019.07493829
AATACGA9000.018.33313639
TCTAGCG9050.018.23184628
TACGAAT9000.018.08869441
CGAATGC9450.017.46012743
GAGCGAA10150.017.33971216
AGCGAAA10300.017.08719317
GGCGTTA8300.016.9636742
GCGAAAG10600.016.81113618
TAGGACG3450.016.5801644
GTATTAC2550.016.3960591
CGCCGGT10200.016.391987
CGGTCCA10700.016.24281310
GCAATAC10800.016.09241937
CAGAGCG11400.016.01736814
Produced by FastQC (version 0.11.5)