##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062689_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2611155
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.181634564014775	32.0	32.0	32.0	32.0	32.0
2	31.345398492238107	32.0	32.0	32.0	32.0	32.0
3	31.464323642219632	32.0	32.0	32.0	32.0	32.0
4	31.583805250933015	32.0	32.0	32.0	32.0	32.0
5	31.509402927057184	32.0	32.0	32.0	32.0	32.0
6	35.08594970424965	36.0	36.0	36.0	36.0	36.0
7	35.09770580451946	36.0	36.0	36.0	36.0	36.0
8	35.044352403438324	36.0	36.0	36.0	36.0	36.0
9	35.16227608089141	36.0	36.0	36.0	36.0	36.0
10	35.05312591554312	36.0	36.0	36.0	36.0	36.0
11	35.177664673295915	36.0	36.0	36.0	36.0	36.0
12	35.097860525323085	36.0	36.0	36.0	36.0	36.0
13	35.1389277924903	36.0	36.0	36.0	36.0	36.0
14	35.09520269765678	36.0	36.0	36.0	36.0	36.0
15	35.05914317610406	36.0	36.0	36.0	36.0	36.0
16	35.06899360627768	36.0	36.0	36.0	36.0	36.0
17	35.04559246770108	36.0	36.0	36.0	36.0	36.0
18	35.055339495357416	36.0	36.0	36.0	36.0	36.0
19	35.03340897035986	36.0	36.0	36.0	36.0	36.0
20	35.028293609532945	36.0	36.0	36.0	36.0	36.0
21	35.016238024935326	36.0	36.0	36.0	36.0	36.0
22	35.004583795293655	36.0	36.0	36.0	36.0	36.0
23	34.96714097784314	36.0	36.0	36.0	36.0	36.0
24	34.95003437176269	36.0	36.0	36.0	32.0	36.0
25	34.91341226392152	36.0	36.0	36.0	32.0	36.0
26	34.868333362056255	36.0	36.0	36.0	32.0	36.0
27	34.85428632157034	36.0	36.0	36.0	32.0	36.0
28	34.835617188562146	36.0	36.0	36.0	32.0	36.0
29	34.81252855537109	36.0	36.0	36.0	32.0	36.0
30	34.80022518770429	36.0	36.0	36.0	32.0	36.0
31	34.794168480997875	36.0	36.0	36.0	32.0	36.0
32	34.75855052649115	36.0	36.0	36.0	32.0	36.0
33	34.72085839408231	36.0	36.0	36.0	32.0	36.0
34	34.70148612395664	36.0	36.0	36.0	32.0	36.0
35	34.67668368978479	36.0	36.0	36.0	32.0	36.0
36	34.647122824956774	36.0	36.0	36.0	32.0	36.0
37	34.63553408357604	36.0	36.0	36.0	32.0	36.0
38	34.59496659524233	36.0	36.0	36.0	32.0	36.0
39	34.57525692653251	36.0	36.0	36.0	32.0	36.0
40	34.557439140916564	36.0	36.0	36.0	32.0	36.0
41	34.52589256478455	36.0	36.0	36.0	32.0	36.0
42	34.48340638529693	36.0	36.0	36.0	32.0	36.0
43	34.465019885835964	36.0	36.0	36.0	32.0	36.0
44	34.427232393327856	36.0	36.0	36.0	32.0	36.0
45	34.369965781426224	36.0	36.0	36.0	32.0	36.0
46	34.37091861647432	36.0	36.0	36.0	32.0	36.0
47	34.30755049010878	36.0	36.0	36.0	32.0	36.0
48	34.258818415605354	36.0	36.0	36.0	32.0	36.0
49	34.240449532869555	36.0	36.0	36.0	32.0	36.0
50	33.720971370906746	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	4.0
20	34.0
21	108.0
22	316.0
23	839.0
24	2152.0
25	4960.0
26	9839.0
27	18146.0
28	29572.0
29	44362.0
30	62457.0
31	87737.0
32	128746.0
33	206898.0
34	450400.0
35	1564582.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80848634439257	18.168241496055362	12.721855981344888	25.30141617820718
2	15.862827808233762	19.98327112346202	37.92778701627772	26.2261140520265
3	19.013521643936553	23.96847056102165	28.167200084866916	28.850807710174877
4	12.509071662978002	15.91473192110918	35.73674158503925	35.839454830873564
5	14.557816199274342	36.52009801030503	32.890208696997576	16.03187709342306
6	34.32288796353778	35.09109619128189	16.61200984550432	13.974005999676004
7	30.56399584245127	30.423483256828206	20.011190454488705	19.00133044623182
8	28.20544931266049	32.95622052310185	18.86758924690415	19.97074091733352
9	27.059443043404162	15.145022030480765	18.78701187788546	39.00852304822961
10	16.47439543037468	26.548558013599344	30.497844823459353	26.479201732566622
11	36.94954914587606	21.658461485434607	21.921869823890194	19.470119544799143
12	25.243043787136344	23.716439659844017	27.943304782749394	23.097211770270246
13	29.293703361156272	19.491374506683822	25.39703694342159	25.817885188738316
14	23.84308859489383	19.853704586667586	25.05408526112008	31.249121557318503
15	25.315923413202203	26.83800846751725	22.65246605429398	25.19360206498657
16	25.81106828204377	26.132573516317493	23.615526462427546	24.44083173921119
17	23.96008662833114	26.12659914865261	25.037655750041647	24.875658472974603
18	24.915181212911527	24.796612993100755	26.321493745105133	23.96671204888258
19	25.307344833991085	25.18938936983825	25.828302034923244	23.674963761247415
20	25.611156748641882	24.140045305621456	25.67656841512664	24.572229530610016
21	26.731618766407966	24.100943835199367	24.893236900911667	24.274200497481
22	25.55221731379409	24.2647410820116	25.451533899749347	24.73150770444497
23	24.136675149502807	24.08734831903889	26.003511855864552	25.77246467559375
24	24.358875669962142	25.20286999431286	25.639688183964566	24.79856615176043
25	24.868420296765226	24.439491336209453	25.648917816062237	25.04317055096308
26	24.34056959468128	25.134394549538424	26.165087863416765	24.35994799236353
27	25.16993437769876	24.767583693805996	25.380530837885917	24.681951090609328
28	24.248886029362485	24.655296219489074	26.156317798062545	24.939499953085896
29	24.133764560127606	24.90775155055904	26.20101066386331	24.757473225450042
30	24.414406651462667	24.957844325595378	26.257882048365573	24.369866974576386
31	24.82269340579169	24.82751885659794	25.173878992246728	25.17590874536364
32	24.438840283322897	24.944631781721117	25.29279188711509	25.3237360478409
33	24.110058575611177	24.581612351622177	25.802566297289896	25.505762775476754
34	24.753490313673453	24.764213537687347	26.020132853086086	24.462163295553115
35	25.43311293278262	24.606582144683102	25.715784777234596	24.244520145299685
36	24.36067563970733	25.21841866913301	25.44081067573545	24.980095015424208
37	25.429053426548787	24.8943858177703	25.29650671829133	24.380054037389584
38	24.353858733012785	24.761379542769387	25.75542240885738	25.129339315360443
39	24.983235388171135	24.774477194957786	25.240860845104944	25.00142657176613
40	25.115437421371002	24.973048325357937	25.49795014083806	24.413564112433004
41	24.09167590587307	25.35326321110773	26.047094102035306	24.507966780983896
42	25.54367703181159	25.304127866786917	25.331050818507517	23.821144282893968
43	24.71952067188658	24.40766633922536	25.558766139888288	25.314046848999773
44	24.314182804161376	25.00586905028618	25.424840731400472	25.255107414151972
45	24.532094035015156	25.16572168255044	25.38765412240943	24.91453016002497
46	24.44255426055406	24.837437589783203	25.545592729030226	25.174415420632513
47	24.541486254736412	24.866476661276966	25.849911571665245	24.742125512321373
48	25.193755253901053	25.67005788626106	24.721933397289707	24.41425346254818
49	24.37549666718368	25.329825307191644	25.242660814850133	25.05201721077454
50	24.29029463601151	25.814295135407562	24.916186483064575	24.97922374551635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	39.0
2	50.0
3	171.5
4	293.0
5	348.5
6	404.0
7	404.0
8	404.0
9	557.5
10	711.0
11	1121.0
12	1531.0
13	2859.0
14	4187.0
15	6148.0
16	8109.0
17	9403.0
18	10697.0
19	11205.5
20	11714.0
21	12747.5
22	13781.0
23	14557.0
24	15333.0
25	16588.0
26	17843.0
27	21670.0
28	25497.0
29	29816.0
30	34135.0
31	38142.0
32	42149.0
33	48272.5
34	54396.0
35	62138.0
36	69880.0
37	82466.0
38	95052.0
39	103885.0
40	112718.0
41	123019.0
42	133320.0
43	138647.0
44	143974.0
45	158582.5
46	173191.0
47	185920.5
48	198650.0
49	201824.0
50	204998.0
51	195711.0
52	186424.0
53	182908.0
54	179392.0
55	180539.0
56	181686.0
57	175633.5
58	169581.0
59	155304.0
60	141027.0
61	124660.0
62	108293.0
63	94111.5
64	79930.0
65	68250.0
66	56570.0
67	49407.5
68	42245.0
69	39390.0
70	36535.0
71	28065.5
72	19596.0
73	17429.5
74	15263.0
75	10963.0
76	6663.0
77	6099.0
78	5535.0
79	4733.0
80	3931.0
81	3089.0
82	2247.0
83	1911.0
84	1575.0
85	1250.0
86	925.0
87	683.5
88	442.0
89	290.5
90	139.0
91	92.5
92	46.0
93	33.0
94	20.0
95	18.0
96	16.0
97	17.5
98	19.0
99	14.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015165702533936131
2	0.0038680200907261343
3	3.063778289684067E-4
4	7.659445724210168E-5
5	3.829722862105084E-5
6	5.361612006947117E-4
7	3.829722862105084E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.5318891448420335E-4
47	3.829722862105084E-5
48	0.0
49	0.0
50	3.829722862105084E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2611155.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.311356332461976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89678462237339	41.52783242156393
2	13.456579087750994	14.34776965526099
3	3.8874706658442837	6.217390016964535
4	1.597176234914166	3.405905255409964
5	0.8275070248421638	2.205776093448803
6	0.4935000978005008	1.57854957383684
7	0.3296034304420386	1.2300124150088154
8	0.21981745853338974	0.9375013487975781
9	0.17516313063281455	0.8404365666128008
>10	0.9576659180881938	9.159101937109053
>50	0.07257803565471813	2.7046359085680494
>100	0.07103556073333747	8.17088776343136
>500	0.010272057413428634	3.7027028927043024
>1k	0.004846674976754883	3.971498151283095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2943	0.11270874383175261	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2702	0.10347911173407938	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2661	0.10190892536061627	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2617	0.10022384730129005	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.829722862105084E-5
2	0.0	0.0	0.0	0.0	3.829722862105084E-5
3	0.0	0.0	0.0	0.0	3.829722862105084E-5
4	0.0	0.0	0.0	0.0	3.829722862105084E-5
5	0.0	0.0	0.0	0.0	3.829722862105084E-5
6	0.0	0.0	0.0	0.0	3.829722862105084E-5
7	0.0	0.0	0.0	0.0	3.829722862105084E-5
8	0.0	0.0	0.0	7.659445724210168E-5	3.829722862105084E-5
9	0.0	0.0	0.0	1.914861431052542E-4	3.829722862105084E-5
10	0.0	0.0	0.0	2.680806003473559E-4	3.829722862105084E-5
11	0.0	0.0	0.0	3.829722862105084E-4	3.829722862105084E-5
12	0.0	0.0	0.0	4.212695148315592E-4	3.063778289684067E-4
13	0.0	0.0	0.0	4.212695148315592E-4	4.212695148315592E-4
14	0.0	0.0	0.0	4.212695148315592E-4	4.212695148315592E-4
15	0.0	0.0	0.0	4.978639720736609E-4	4.978639720736609E-4
16	0.0	0.0	0.0	6.127556579368134E-4	4.978639720736609E-4
17	0.0	0.0	0.0	6.89350115178915E-4	5.361612006947118E-4
18	0.0	0.0	0.0	9.191334869052201E-4	6.127556579368134E-4
19	0.0	0.0	0.0	9.574307155262709E-4	6.510528865578643E-4
20	0.0	0.0	0.0	0.001148916858631525	7.276473437999659E-4
21	0.0	0.0	0.0	0.0014935919162209826	7.276473437999659E-4
22	0.0	0.0	0.0	0.0024510226317472537	7.276473437999659E-4
23	0.0	0.0	0.0	0.004212695148315592	7.276473437999659E-4
24	0.0	0.0	0.0	0.006625420551441795	7.276473437999659E-4
25	0.0	0.0	0.0	0.008157309696283829	7.276473437999659E-4
26	0.0	0.0	0.0	0.010455143413546879	7.659445724210168E-4
27	0.0	0.0	0.0	0.01282957158805203	8.042418010420676E-4
28	0.0	0.0	0.0	0.0183826697381044	8.042418010420676E-4
29	0.0	0.0	0.0	0.027574004607156602	8.042418010420676E-4
30	0.0	0.0	0.0	0.043237571113166395	8.042418010420676E-4
31	0.0	0.0	0.0	0.08544111705356441	8.042418010420676E-4
32	0.0	0.0	0.0	0.13388711125919373	8.042418010420676E-4
33	0.0	0.0	0.0	0.1765119267144233	8.042418010420676E-4
34	0.0	0.0	0.0	0.22549408212074734	8.425390296631184E-4
35	0.0	0.0	0.0	0.2830931139668078	8.425390296631184E-4
36	0.0	0.0	0.0	0.3615258381827199	8.425390296631184E-4
37	0.0	0.0	0.0	0.47151547878237793	8.425390296631184E-4
38	0.0	0.0	0.0	0.6113769577064556	8.425390296631184E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	360	0.0	21.388657	15
CGCAATA	815	0.0	20.245178	36
ATACGAA	835	0.0	19.760265	40
TAGCGGC	865	0.0	19.32927	30
CAATACG	875	0.0	19.108364	38
CTAGCGG	865	0.0	19.074938	29
AATACGA	900	0.0	18.333136	39
TCTAGCG	905	0.0	18.231846	28
TACGAAT	900	0.0	18.088694	41
CGAATGC	945	0.0	17.460127	43
GAGCGAA	1015	0.0	17.339712	16
AGCGAAA	1030	0.0	17.087193	17
GGCGTTA	830	0.0	16.96367	42
GCGAAAG	1060	0.0	16.811136	18
TAGGACG	345	0.0	16.580164	4
GTATTAC	255	0.0	16.396059	1
CGCCGGT	1020	0.0	16.39198	7
CGGTCCA	1070	0.0	16.242813	10
GCAATAC	1080	0.0	16.092419	37
CAGAGCG	1140	0.0	16.017368	14
>>END_MODULE