FastQCFastQC Report
Thu 2 Feb 2017
SRR4062688_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062688_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846920
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21330.25185377603551695No Hit
TATCAACGCAGAGTACTTTTTTTTT14460.17073631511831106No Hit
GTACATGGAAGCAGTGGTATCAACG11940.14098143862466347No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11320.1336607944079724No Hit
TCCATGTACTCTGCGTTGATACCAC10340.12208945354933169No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10000.11807490672082369No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9870.11653993293345298No Hit
GCGCAAGACGGACCAGAGCGAAAGC9720.11476880933264062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACA350.00217162616.2828774
CGTTATT551.9672971E-413.8108752
GCGTTAT551.9722346E-413.8067941
GTAAGGG707.2751336E-613.5674593
GTTTAAG500.001507901413.2890411
TATACAG1153.765308E-1013.215875
GTCCTAC1600.013.0517361
GTATTAG1251.03682396E-1012.9093531
CGTCGTA1051.9901563E-812.665956510
CGACCCG604.092574E-412.6652085
CCGTCGT1155.307811E-912.3913439
AATTCCG853.942385E-612.2927025
AACCGCG701.0897349E-412.2143237
TTTATAC701.09274675E-412.2107133
CTTACAC701.09274675E-412.2107133
CGAACGA802.8522712E-511.879251516
GTCTTAG802.8870001E-511.8652141
TCCTACA1850.011.8045652
CGACTTT907.4440395E-611.61183513
TTCCGAT907.4489417E-611.6111477
Produced by FastQC (version 0.11.5)