##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062688_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	846920
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11251475936334	32.0	32.0	32.0	32.0	32.0
2	30.902888112218392	32.0	32.0	32.0	32.0	32.0
3	30.88605535351627	32.0	32.0	32.0	32.0	32.0
4	30.91907027818448	32.0	32.0	32.0	32.0	32.0
5	30.787715486704766	32.0	32.0	32.0	32.0	32.0
6	34.27761535918387	36.0	36.0	36.0	32.0	36.0
7	34.21806782222642	36.0	36.0	36.0	32.0	36.0
8	34.23541184527464	36.0	36.0	36.0	32.0	36.0
9	34.35918386624475	36.0	36.0	36.0	32.0	36.0
10	34.131536626836066	36.0	36.0	36.0	32.0	36.0
11	34.33391111321022	36.0	36.0	36.0	32.0	36.0
12	34.168718414962456	36.0	36.0	36.0	32.0	36.0
13	34.26982123459123	36.0	36.0	36.0	32.0	36.0
14	34.197078826807726	36.0	36.0	36.0	32.0	36.0
15	34.127517357011286	36.0	36.0	36.0	32.0	36.0
16	34.13391229395929	36.0	36.0	36.0	32.0	36.0
17	34.06870660747178	36.0	36.0	36.0	32.0	36.0
18	34.08152718084353	36.0	36.0	36.0	32.0	36.0
19	34.06958036178151	36.0	36.0	36.0	32.0	36.0
20	34.04127072214613	36.0	36.0	36.0	32.0	36.0
21	34.01601449959855	36.0	36.0	36.0	32.0	36.0
22	33.97661408397487	36.0	36.0	36.0	32.0	36.0
23	33.95359538090965	36.0	36.0	36.0	32.0	36.0
24	33.92564941198697	36.0	36.0	36.0	32.0	36.0
25	33.40471118877816	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	38.0
5	78.0
6	139.0
7	50.0
8	115.0
9	149.0
10	94.0
11	33.0
12	66.0
13	47.0
14	152.0
15	263.0
16	413.0
17	585.0
18	728.0
19	952.0
20	1220.0
21	1555.0
22	2022.0
23	2912.0
24	3992.0
25	5540.0
26	8008.0
27	10904.0
28	15211.0
29	21442.0
30	30061.0
31	43669.0
32	66426.0
33	99919.0
34	222604.0
35	307531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.29697738321708	17.501585288522513	11.17436520560336	26.027072122657053
2	16.969183864804805	19.923508727184274	36.84085611658602	26.266451291424904
3	19.599609859317916	23.022787333180613	28.57142857142857	28.806174236072902
4	13.04400710915865	14.700842588820198	35.39522552683943	36.85992477518171
5	14.792097054053702	35.786024679283884	33.70410136294753	15.717776903714887
6	34.75845938491161	34.62568439952985	16.40586147221943	14.209994743339102
7	30.000070891986606	30.432984623528103	20.528901481406216	19.038043003079075
8	27.842774386136576	32.98518786383329	19.730961311655193	19.441076438374942
9	26.820937680971007	14.414924420597309	18.895205228510985	39.868932669920696
10	15.69524007669593	26.612588629426742	31.852334331830466	25.839836962046864
11	37.27223292707434	21.103637416807537	22.208338751433335	19.415790904684783
12	24.098149006403283	23.654790167128546	28.989401950305975	23.25765887616219
13	29.33662281349853	19.219225254128894	25.58973625446107	25.8544156779115
14	23.068647710560786	19.601387424280396	25.29342084730294	32.03654401785588
15	24.888174910516287	27.381425214311722	22.586658533932884	25.143741341239107
16	25.336444521672526	25.92475699751412	24.300248587730902	24.438549893082453
17	23.7477907753418	25.989703092027877	25.817221017041327	24.445285115588998
18	24.486077876876227	24.99988177544902	26.86936660014565	23.644673747529108
19	25.05027528749425	25.235770704492445	25.77771524975675	23.936238758256557
20	24.981529008027938	24.61766524297186	25.902064140741277	24.49874160825893
21	25.653933413174784	24.640270121050833	25.061506686229006	24.644289779545375
22	25.03426232823015	24.783813281840317	25.533504950448215	24.648419439481316
23	23.938736131210806	24.493960632974847	25.885450658797044	25.6818525770173
24	24.298965976292884	25.196672585957803	25.708955312110586	24.795406125638728
25	24.453534631049422	24.66113767888916	25.90498259137312	24.980345098688296
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	85.0
2	259.5
3	434.0
4	434.0
5	434.0
6	1137.5
7	1841.0
8	1841.0
9	1841.0
10	2019.0
11	2197.0
12	2197.0
13	2197.0
14	2335.0
15	2473.0
16	2473.0
17	2473.0
18	4160.5
19	5848.0
20	5848.0
21	5848.0
22	9440.5
23	13033.0
24	13033.0
25	13033.0
26	19011.5
27	24990.0
28	24990.0
29	24990.0
30	31650.0
31	38310.0
32	38310.0
33	38310.0
34	46033.0
35	53756.0
36	53756.0
37	53756.0
38	61267.0
39	68778.0
40	68778.0
41	68778.0
42	78648.0
43	88518.0
44	88518.0
45	88518.0
46	100293.0
47	112068.0
48	112068.0
49	112068.0
50	112564.5
51	113061.0
52	113061.0
53	113061.0
54	104847.0
55	96633.0
56	96633.0
57	96633.0
58	88641.0
59	80649.0
60	80649.0
61	80649.0
62	70972.5
63	61296.0
64	61296.0
65	61296.0
66	50248.5
67	39201.0
68	39201.0
69	39201.0
70	30017.0
71	20833.0
72	20833.0
73	20833.0
74	15912.5
75	10992.0
76	10992.0
77	10992.0
78	8997.5
79	7003.0
80	7003.0
81	7003.0
82	4897.0
83	2791.0
84	2791.0
85	2791.0
86	2027.0
87	1263.0
88	1263.0
89	1263.0
90	852.5
91	442.0
92	442.0
93	442.0
94	257.5
95	73.0
96	73.0
97	73.0
98	212.5
99	352.0
100	352.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008383318377178482
2	0.003069947574741416
3	0.0054314457091578896
4	0.01475936334010296
5	0.035304397109526285
6	0.04427809002030888
7	0.06635809757710291
8	0.08525008265243471
9	0.09327917630945072
10	0.11594955839984887
11	0.11689415765361545
12	0.13035469701978936
13	0.11701223256033629
14	0.12232560336277334
15	0.11287961082510745
16	0.11110848722429509
17	0.12220752845605252
18	0.126576300004723
19	0.1271666745383271
20	0.11795683181410287
21	0.1271666745383271
22	0.14629480942710055
23	0.1341330940348557
24	0.1296462475794644
25	0.12693052472488547
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	846920.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.496659123074835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.12661339997426	50.288823949138674
2	10.151687362782384	12.282863398205448
3	2.8105009156162395	5.100777475713761
4	1.2324352048823533	2.9823284992417842
5	0.6528930360533692	1.9748923722975043
6	0.4270151078367351	1.5499792451521202
7	0.2967036159571318	1.2564704260599615
8	0.20296085859748156	0.9822763102318747
9	0.14946213180893309	0.8137763675867795
>10	0.797237814413317	8.671473312076335
>50	0.07654033457548036	3.2354759688879353
>100	0.07105651793485762	8.398298191803901
>500	0.003914959653998841	1.6408412351437758
>1k	9.787399134997103E-4	0.821723248460065
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2133	0.25185377603551695	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1446	0.17073631511831106	No Hit
GTACATGGAAGCAGTGGTATCAACG	1194	0.14098143862466347	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1132	0.1336607944079724	No Hit
TCCATGTACTCTGCGTTGATACCAC	1034	0.12208945354933169	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1000	0.11807490672082369	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	987	0.11653993293345298	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	972	0.11476880933264062	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3614981344164738E-4
2	0.0	0.0	0.0	0.0	2.3614981344164738E-4
3	0.0	0.0	0.0	0.0	2.3614981344164738E-4
4	0.0	0.0	0.0	0.0	3.542247201624711E-4
5	0.0	0.0	0.0	0.0	3.542247201624711E-4
6	0.0	0.0	0.0	0.0	3.542247201624711E-4
7	0.0	0.0	0.0	0.0	3.542247201624711E-4
8	0.0	0.0	0.0	0.0	3.542247201624711E-4
9	0.0	0.0	0.0	0.0	4.7229962688329476E-4
10	0.0	0.0	0.0	0.0	4.7229962688329476E-4
11	0.0	0.0	0.0	0.0	4.7229962688329476E-4
12	0.0	0.0	0.0	0.0	5.903745336041184E-4
13	0.0	0.0	0.0	0.0	5.903745336041184E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCACA	35	0.002171626	16.282877	4
CGTTATT	55	1.9672971E-4	13.810875	2
GCGTTAT	55	1.9722346E-4	13.806794	1
GTAAGGG	70	7.2751336E-6	13.567459	3
GTTTAAG	50	0.0015079014	13.289041	1
TATACAG	115	3.765308E-10	13.21587	5
GTCCTAC	160	0.0	13.051736	1
GTATTAG	125	1.03682396E-10	12.909353	1
CGTCGTA	105	1.9901563E-8	12.6659565	10
CGACCCG	60	4.092574E-4	12.665208	5
CCGTCGT	115	5.307811E-9	12.391343	9
AATTCCG	85	3.942385E-6	12.292702	5
AACCGCG	70	1.0897349E-4	12.214323	7
TTTATAC	70	1.09274675E-4	12.210713	3
CTTACAC	70	1.09274675E-4	12.210713	3
CGAACGA	80	2.8522712E-5	11.8792515	16
GTCTTAG	80	2.8870001E-5	11.865214	1
TCCTACA	185	0.0	11.804565	2
CGACTTT	90	7.4440395E-6	11.611835	13
TTCCGAT	90	7.4489417E-6	11.611147	7
>>END_MODULE