FastQCFastQC Report
Thu 2 Feb 2017
SRR4062688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846920
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9000.10626741604874133No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8660.10225286922023331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAG300.00574420629.33317617
CGAATGC2200.021.99988243
GTAAGGC602.8718988E-421.9998823
ATACGAA2200.021.99988240
CAATACG2250.021.51099638
CGCAATA2300.021.04336436
AATACGA2350.020.59563639
TACGAAT2400.020.16655741
TTAGGCA1101.2645796E-719.9998934
CGTAAAT550.00448266219.9998933
TAGGACG1851.8189894E-1219.0269264
TAACGCC2550.018.9802914
GCAATAC2550.018.98029137
TCTAGCG2600.018.61528428
TTATAAC600.00741054618.3332353
TCGGTTG600.00741054618.33323517
CTAGCGG2650.018.26405329
GTGTACG851.4283114E-418.120761
CGAGCCG2550.018.1175515
AGGACGT1951.8189894E-1218.0511865
Produced by FastQC (version 0.11.5)