FastQCFastQC Report
Thu 2 Feb 2017
SRR4062687_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062687_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536755
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG44160.822721725927099No Hit
TCCATGTACTCTGCGTTGATACCAC37030.689886447261786No Hit
GTATCAACGCAGAGTACTTTTTTTT27810.5181134782163185No Hit
GAGTACATGGAAGCAGTGGTATCAA19750.36795185885553No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18200.3390746243630707No Hit
TATCAACGCAGAGTACTTTTTTTTT17530.32659220687278184No Hit
CATGTACTCTGCGTTGATACCACTG16930.3154139225531202No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15650.29156691600450857No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11740.21872176318804668No Hit
GCGTTGATACCACTGCTTCCATGTA10680.19897346088997772No Hit
CATGGAAGCAGTGGTATCAACGCAG10490.1954336708554182No Hit
GCTTCCATGTACTCTGCGTTGATAC10360.19301170925282485No Hit
GGTATCAACGCAGAGTACTTTTTTT9600.17885254911458673No Hit
GTACATGGGGTGGTATCAACGCAAA8290.15444662834999207No Hit
GTATCAACGCAGAGTACATGGAAGC8230.15332879991802592No Hit
CCCATGTACTCTGCGTTGATACCAC7430.1384244208251437No Hit
ACTCTGCGTTGATACCACTGCTTCC6290.11718568061778652No Hit
GCGCAAGACGGACCAGAGCGAAAGC6060.11290067162858287No Hit
CAGTGGTATCAACGCAGAGTACATG6000.1117828431966167No Hit
GTCTTGCGCCGGTCCAAGAATTTCA5960.1110376242419726No Hit
ACGCAGAGTACATGGAAGCAGTGGT5750.10712522473009102No Hit
GAATAACGCCGCCGCATCGCCAGTC5750.10712522473009102No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG5730.10675261525276895No Hit
GAATAGGACCGCGGTTCTATTTTGT5720.10656631051410793No Hit
GTCCTATTCCATTATTCCTAGCTGC5490.10228130152490429No Hit
GTATCTGATCGTCTTCGAACCTCCG5480.10209499678624324No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5420.1009771683542771No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA453.5278994E-516.89112916
TAATTCC1151.8189894E-1214.8701494
AACAATA456.7740114E-414.7714692
ATAAGAC400.005278290214.2479023
CTTACAC400.005278290214.2479023
TCTAGAT759.685264E-713.9273852
AAGACGG1301.8189894E-1213.885165
GACGGTA1257.2759576E-1213.6805388
TCCTATT1400.013.5656352
GTTATTC852.6927046E-713.409793
GTCCTAC500.001502140713.2943221
TCCGATA655.4374977E-513.1543638
CCGATAA655.4374977E-513.1543639
CCTATTC1450.013.101523
CGAGGAT801.9894796E-613.06423216
ACGAGGA801.9915642E-613.06301315
CGCCAGT1102.7193892E-912.95747118
CTATTCC1403.6379788E-1212.8933634
TACGACG1352.7284841E-1112.6671655
ACGACGG1352.7284841E-1112.6671656
Produced by FastQC (version 0.11.5)