##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062687_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	536755
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.829296420154446	32.0	32.0	32.0	32.0	32.0
2	30.528360238842676	32.0	32.0	32.0	32.0	32.0
3	30.58034112397649	32.0	32.0	32.0	32.0	32.0
4	30.41857644549189	32.0	32.0	32.0	27.0	32.0
5	30.48935175266183	32.0	32.0	32.0	32.0	32.0
6	33.92472915948617	36.0	36.0	36.0	32.0	36.0
7	33.76367989119803	36.0	36.0	36.0	32.0	36.0
8	33.77091783029501	36.0	36.0	36.0	32.0	36.0
9	33.816596026119925	36.0	36.0	36.0	32.0	36.0
10	33.56585220445082	36.0	36.0	36.0	27.0	36.0
11	33.93632662946782	36.0	36.0	36.0	32.0	36.0
12	33.69545695894775	36.0	36.0	36.0	32.0	36.0
13	33.813281664819144	36.0	36.0	36.0	32.0	36.0
14	33.71757133142682	36.0	36.0	36.0	32.0	36.0
15	33.719467913666385	36.0	36.0	36.0	32.0	36.0
16	33.68771972315116	36.0	36.0	36.0	27.0	36.0
17	33.59898277612691	36.0	36.0	36.0	27.0	36.0
18	33.65520768320742	36.0	36.0	36.0	27.0	36.0
19	33.5826773853993	36.0	36.0	36.0	27.0	36.0
20	33.576000223565686	36.0	36.0	36.0	27.0	36.0
21	33.46764538756043	36.0	36.0	36.0	27.0	36.0
22	33.421548006073536	36.0	36.0	36.0	27.0	36.0
23	33.397132770072005	36.0	36.0	36.0	27.0	36.0
24	33.36054251939898	36.0	36.0	36.0	27.0	36.0
25	32.686862721353314	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	25.0
5	51.0
6	80.0
7	26.0
8	82.0
9	105.0
10	49.0
11	23.0
12	33.0
13	33.0
14	377.0
15	741.0
16	1095.0
17	1505.0
18	1703.0
19	1850.0
20	2117.0
21	2265.0
22	2642.0
23	2929.0
24	3703.0
25	4687.0
26	6210.0
27	8211.0
28	11097.0
29	15175.0
30	20496.0
31	29629.0
32	45042.0
33	66854.0
34	140356.0
35	167562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.00250040058579	16.72920773447311	11.142992357195824	25.12529950774528
2	16.79729928625591	20.560524791009545	35.969646779581446	26.672529143153106
3	19.36034997046938	25.384037814100544	27.60954742692336	27.64606478850672
4	12.848978800871294	15.006344645622194	34.64586442768688	37.49881212581963
5	14.679193169937138	36.54851472969926	32.7353984296521	16.036893670711503
6	34.25345885808756	34.12727554000682	17.11806248031298	14.501203121592631
7	29.83924340185794	30.4423369829661	21.241571138010556	18.476848477165404
8	29.29796755547268	30.767480887562932	20.17079992541488	19.763751631549507
9	27.89361499773423	14.057858313705966	18.503247606952844	39.545279081606964
10	16.431994061219427	26.110303896205632	30.860395757054633	26.597306285520304
11	37.625110515065494	21.36904384417096	21.130480521369975	19.875365119393575
12	24.62041857957545	23.687671508867627	28.479566309922006	23.21234360163492
13	30.11064769564796	19.33033149542263	25.442255034358237	25.116765774571174
14	24.589760282341178	18.807884511713542	24.831511185642682	31.770844020302597
15	25.72655279561028	25.581447398360947	23.969434611180947	24.722565194847824
16	26.33118160844078	25.35050236963289	23.670403742173495	24.647912279752834
17	23.892682799439648	25.078671051478008	25.920129529764424	25.108516619317918
18	25.30867767130468	23.604444883850643	27.24851094152519	23.838366503319484
19	25.167052818911383	25.915292748172774	25.354532531927514	23.563121900988328
20	25.210079331178292	22.950082726022977	26.914030221578898	24.92580772121983
21	26.58548278480115	24.16470329268543	24.0675139488161	25.18229997369732
22	25.382739453557214	24.27944224995755	25.08102601228871	25.25679228419652
23	23.652303048281087	24.087371993276207	25.730260985397578	26.530063973045127
24	23.89605059418316	25.82150252784359	25.373766393672	24.90868048430125
25	25.2319258285064	24.10853363927209	24.830847580938524	25.82869295128298
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118.0
1	118.0
2	370.0
3	622.0
4	622.0
5	622.0
6	1522.0
7	2422.0
8	2422.0
9	2422.0
10	2444.5
11	2467.0
12	2467.0
13	2467.0
14	1957.0
15	1447.0
16	1447.0
17	1447.0
18	2419.5
19	3392.0
20	3392.0
21	3392.0
22	5257.0
23	7122.0
24	7122.0
25	7122.0
26	10373.5
27	13625.0
28	13625.0
29	13625.0
30	16425.5
31	19226.0
32	19226.0
33	19226.0
34	23997.5
35	28769.0
36	28769.0
37	28769.0
38	33301.5
39	37834.0
40	37834.0
41	37834.0
42	46292.0
43	54750.0
44	54750.0
45	54750.0
46	69596.0
47	84442.0
48	84442.0
49	84442.0
50	79015.0
51	73588.0
52	73588.0
53	73588.0
54	66500.5
55	59413.0
56	59413.0
57	59413.0
58	54576.5
59	49740.0
60	49740.0
61	49740.0
62	44975.5
63	40211.0
64	40211.0
65	40211.0
66	33490.5
67	26770.0
68	26770.0
69	26770.0
70	20482.0
71	14194.0
72	14194.0
73	14194.0
74	11065.5
75	7937.0
76	7937.0
77	7937.0
78	6468.0
79	4999.0
80	4999.0
81	4999.0
82	3493.0
83	1987.0
84	1987.0
85	1987.0
86	1447.5
87	908.0
88	908.0
89	908.0
90	583.5
91	259.0
92	259.0
93	259.0
94	152.5
95	46.0
96	46.0
97	46.0
98	256.5
99	467.0
100	467.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007638494285102141
2	0.001490437909288223
3	0.004471313727864669
4	0.015276988570204281
5	0.03465268139095118
6	0.04359530884668052
7	0.06595187748600385
8	0.08476865609076767
9	0.09576063567176832
10	0.11513632849251522
11	0.11718568061778652
12	0.1311585360173636
13	0.1190487280043968
14	0.12407895594824453
15	0.11047871002598951
16	0.1110376242419726
17	0.12333373699360042
18	0.12631461281217687
19	0.1298544028467364
20	0.12072547065234604
21	0.12817766019878715
22	0.15202466674739873
23	0.13935594451844882
24	0.13507093552924518
25	0.1304133170627195
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	536755.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.30892206683746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69954552562606	40.37988848069875
2	12.615407352172964	12.945659053481018
3	3.749585550099953	5.77161578319055
4	1.5676911172610342	3.21746165441679
5	0.8550882344578379	2.193682779103342
6	0.5162590687415846	1.5893217794616028
7	0.32697419373528525	1.1743685396961536
8	0.2320644038602513	0.9525579529722182
9	0.17711638822336181	0.8178885864100979
>10	1.032912626670968	10.126167271037875
>50	0.11313036468686703	4.11808074824512
>100	0.10364201133456787	10.1964300843994
>500	0.006203923213688922	2.0168166089260113
>1k	0.004379239915545121	4.500060677961083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4416	0.822721725927099	No Hit
TCCATGTACTCTGCGTTGATACCAC	3703	0.689886447261786	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2781	0.5181134782163185	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1975	0.36795185885553	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1820	0.3390746243630707	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1753	0.32659220687278184	No Hit
CATGTACTCTGCGTTGATACCACTG	1693	0.3154139225531202	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1565	0.29156691600450857	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1174	0.21872176318804668	No Hit
GCGTTGATACCACTGCTTCCATGTA	1068	0.19897346088997772	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1049	0.1954336708554182	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1036	0.19301170925282485	No Hit
GGTATCAACGCAGAGTACTTTTTTT	960	0.17885254911458673	No Hit
GTACATGGGGTGGTATCAACGCAAA	829	0.15444662834999207	No Hit
GTATCAACGCAGAGTACATGGAAGC	823	0.15332879991802592	No Hit
CCCATGTACTCTGCGTTGATACCAC	743	0.1384244208251437	No Hit
ACTCTGCGTTGATACCACTGCTTCC	629	0.11718568061778652	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	606	0.11290067162858287	No Hit
CAGTGGTATCAACGCAGAGTACATG	600	0.1117828431966167	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	596	0.1110376242419726	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	575	0.10712522473009102	No Hit
GAATAACGCCGCCGCATCGCCAGTC	575	0.10712522473009102	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	573	0.10675261525276895	No Hit
GAATAGGACCGCGGTTCTATTTTGT	572	0.10656631051410793	No Hit
GTCCTATTCCATTATTCCTAGCTGC	549	0.10228130152490429	No Hit
GTATCTGATCGTCTTCGAACCTCCG	548	0.10209499678624324	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	542	0.1009771683542771	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.8630473866102784E-4	0.0
10	0.0	0.0	0.0	1.8630473866102784E-4	0.0
11	0.0	0.0	0.0	3.7260947732205567E-4	0.0
12	0.0	0.0	0.0	3.7260947732205567E-4	0.0
13	0.0	0.0	0.0	7.452189546441113E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	45	3.5278994E-5	16.891129	16
TAATTCC	115	1.8189894E-12	14.870149	4
AACAATA	45	6.7740114E-4	14.771469	2
ATAAGAC	40	0.0052782902	14.247902	3
CTTACAC	40	0.0052782902	14.247902	3
TCTAGAT	75	9.685264E-7	13.927385	2
AAGACGG	130	1.8189894E-12	13.88516	5
GACGGTA	125	7.2759576E-12	13.680538	8
TCCTATT	140	0.0	13.565635	2
GTTATTC	85	2.6927046E-7	13.40979	3
GTCCTAC	50	0.0015021407	13.294322	1
TCCGATA	65	5.4374977E-5	13.154363	8
CCGATAA	65	5.4374977E-5	13.154363	9
CCTATTC	145	0.0	13.10152	3
CGAGGAT	80	1.9894796E-6	13.064232	16
ACGAGGA	80	1.9915642E-6	13.063013	15
CGCCAGT	110	2.7193892E-9	12.957471	18
CTATTCC	140	3.6379788E-12	12.893363	4
TACGACG	135	2.7284841E-11	12.667165	5
ACGACGG	135	2.7284841E-11	12.667165	6
>>END_MODULE