FastQCFastQC Report
Thu 2 Feb 2017
SRR4062687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536755
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21730.40484019711041347No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18550.34559529021620666No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA9370.17456754012538309No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC9010.16786056953358608No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8440.1572411994299075No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC6480.12072547065234604No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5920.11029240528732849No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA5840.10880196737804025No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG5700.10619370103678587No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT5630.10488956786615868No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5530.1030265204795484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGAC300.00574226329.33327123
CGCAATA1900.025.4736336
TTAGTGT450.001397219824.4443914
ATACGAA2100.024.09518840
CTAGCGG2300.022.95647229
AATACGA2250.022.4888439
GTGTATA500.00257722422.0021
TACGAAT2300.021.99995241
TAGCGGC2450.021.55097430
TCTAGCG2350.021.5318728
CAATACG2250.021.51106538
CGAATGC2600.021.15380343
GCAATAC2300.021.04343437
ATACTGA856.0981984E-620.7077671
GCGCAAT2350.020.59570335
AGCGGCG2600.020.3076531
GTATAGA550.00447806120.001821
TTTACAC550.004480476519.9999583
TCATATA550.004480476519.9999582
CGCAAAT1252.2808308E-819.35995923
Produced by FastQC (version 0.11.5)