##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062687_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	536755
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0243910163855	32.0	32.0	32.0	32.0	32.0
2	31.14232750510009	32.0	32.0	32.0	32.0	32.0
3	31.328140399251055	32.0	32.0	32.0	32.0	32.0
4	31.502445249694926	32.0	32.0	32.0	32.0	32.0
5	31.363234622872632	32.0	32.0	32.0	32.0	32.0
6	34.94655475962031	36.0	36.0	36.0	36.0	36.0
7	34.952335795660964	36.0	36.0	36.0	36.0	36.0
8	34.893211986846886	36.0	36.0	36.0	32.0	36.0
9	35.00020679825992	36.0	36.0	36.0	36.0	36.0
10	34.876696071764584	36.0	36.0	36.0	32.0	36.0
11	35.02694152825777	36.0	36.0	36.0	36.0	36.0
12	34.90703766150292	36.0	36.0	36.0	32.0	36.0
13	34.9683598662332	36.0	36.0	36.0	36.0	36.0
14	34.91805758679472	36.0	36.0	36.0	32.0	36.0
15	34.873441327980174	36.0	36.0	36.0	32.0	36.0
16	34.88865124684446	36.0	36.0	36.0	32.0	36.0
17	34.865016627697926	36.0	36.0	36.0	32.0	36.0
18	34.870723141843115	36.0	36.0	36.0	32.0	36.0
19	34.83746960903951	36.0	36.0	36.0	32.0	36.0
20	34.807204404244025	36.0	36.0	36.0	32.0	36.0
21	34.78557069799071	36.0	36.0	36.0	32.0	36.0
22	34.76593231548844	36.0	36.0	36.0	32.0	36.0
23	34.719065495430876	36.0	36.0	36.0	32.0	36.0
24	34.697450419651425	36.0	36.0	36.0	32.0	36.0
25	34.68623487438403	36.0	36.0	36.0	32.0	36.0
26	34.63098806718149	36.0	36.0	36.0	32.0	36.0
27	34.61061378096152	36.0	36.0	36.0	32.0	36.0
28	34.57566114894132	36.0	36.0	36.0	32.0	36.0
29	34.53075984387663	36.0	36.0	36.0	32.0	36.0
30	34.52818324934095	36.0	36.0	36.0	32.0	36.0
31	34.51473018416223	36.0	36.0	36.0	32.0	36.0
32	34.43925254538849	36.0	36.0	36.0	32.0	36.0
33	34.40015835902786	36.0	36.0	36.0	32.0	36.0
34	34.37215675680711	36.0	36.0	36.0	32.0	36.0
35	34.30043502156477	36.0	36.0	36.0	32.0	36.0
36	34.25711358068393	36.0	36.0	36.0	32.0	36.0
37	34.225648573371465	36.0	36.0	36.0	32.0	36.0
38	34.15369582025319	36.0	36.0	36.0	32.0	36.0
39	34.05659379046306	36.0	36.0	36.0	32.0	36.0
40	34.00372609477322	36.0	36.0	36.0	32.0	36.0
41	33.97891402967834	36.0	36.0	36.0	32.0	36.0
42	33.86130916339857	36.0	36.0	36.0	32.0	36.0
43	33.86589598606441	36.0	36.0	36.0	32.0	36.0
44	33.82482696947397	36.0	36.0	36.0	32.0	36.0
45	33.66514145187283	36.0	36.0	36.0	27.0	36.0
46	33.721791133757485	36.0	36.0	36.0	27.0	36.0
47	33.61686057884882	36.0	36.0	36.0	27.0	36.0
48	33.571312796340976	36.0	36.0	36.0	21.0	36.0
49	33.5821203342307	36.0	36.0	36.0	21.0	36.0
50	32.90670044992594	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	13.0
22	53.0
23	173.0
24	454.0
25	1074.0
26	2373.0
27	4566.0
28	8168.0
29	12442.0
30	17789.0
31	25167.0
32	36636.0
33	55544.0
34	109769.0
35	262530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3861963207331	17.493194306508684	11.638527771515028	25.482081601243188
2	16.294195788952006	21.118586405480585	35.37284039863544	27.214377406931966
3	19.071377470414642	25.451791516380005	27.85196887948252	27.62486213372284
4	12.86532166564509	15.32772057165959	34.8955664896144	36.91139127308091
5	14.699816489832418	36.46710324077093	32.553399595718716	16.279680673677934
6	34.491720571139	34.30429695650878	16.684614123468567	14.519368348883654
7	29.73554042347067	30.616389227860008	21.0990116533614	18.549058695307917
8	29.400191893880823	30.671908039981	20.057381859507597	19.870518206630585
9	28.046501662769792	13.807789401123419	18.064852679527903	40.08085625657888
10	16.51256159700422	26.074279699304153	30.803625490214344	26.60953321347728
11	38.143473279242855	21.191977717953257	20.821603897495134	19.842945105308754
12	24.641409954262187	23.479799908710678	28.247524475785042	23.631265661242093
13	30.36972175387281	19.164982161321273	25.36166407392572	25.103632010880194
14	24.717049678158563	18.40877122709616	24.74201451313914	32.13216458160613
15	26.059002710733946	25.394453707930058	23.8028523255489	24.74369125578709
16	26.590157520656536	25.090963288651245	23.534759806615682	24.784119384076533
17	24.169686355972463	24.94844016357556	25.748432711386016	25.133440769065963
18	25.710053935221843	23.163268157725593	27.14664977503703	23.980028132015537
19	25.245223612262578	25.616342651675346	25.348995351696768	23.789438384365305
20	25.353466665424634	22.80928915426964	26.779443135136145	25.057801045169587
21	26.861789829624318	23.92450931989455	24.158880681130125	25.054820169351004
22	25.3326005346946	24.1001946884519	25.0935715549925	25.473633221860997
23	23.98394053152742	23.599221246192396	25.71527046790435	26.701567754375834
24	24.205456865795384	25.522445063390187	25.267207571424578	25.00489049938985
25	25.534368566664494	23.646356345073638	24.946204506711627	25.873070581550238
26	24.064796788106303	24.569496325139028	25.718623953200247	25.647082933554415
27	25.9602611992436	24.72971840038751	25.02836489646114	24.281655503907743
28	24.55496455552347	23.392050376801336	26.205997149537495	25.8469879181377
29	23.94034522268074	25.133813378543284	26.478188372721263	24.447653026054716
30	25.12263509422362	24.311836871570826	26.203202578457585	24.362325455747964
31	24.108205792214324	24.795297668396195	25.230505537908353	25.86599100148112
32	24.34965673351902	24.720962077670443	25.30316438598616	25.62621680282438
33	23.756835055099625	24.27029091484942	25.842330299671172	26.130543730379785
34	24.33978258236998	24.91080660636603	26.252200724725434	24.49721008653855
35	26.196122998388464	24.182355078201414	25.322912688284227	24.298609235125895
36	24.652029324365866	25.52654376764073	24.999860271446003	24.821566636547402
37	26.004042812828942	24.80163202951067	25.337258153161123	23.85706700449926
38	24.405920764594647	24.726923829307598	25.121144656314332	25.746010749783423
39	24.58328287579995	24.60526683496195	25.66366405529525	25.14778623394286
40	25.466181032314555	24.679229816210373	25.099905916106973	24.75468323536809
41	23.558793117902955	25.481458020884762	25.851831841342886	25.1079170198694
42	26.274743598103417	25.385697385212993	24.98290654022785	23.35665247645574
43	25.12393922739425	23.82222801836965	24.807407476409164	26.24642527782694
44	24.480442660059058	24.640478430568884	25.87344319102756	25.0056357183445
45	24.93148643235741	25.030600553325073	24.84839451891459	25.18951849540293
46	24.32031120459504	24.812530158191944	25.779082743831893	25.088075893381127
47	24.935445287785466	24.23344772465599	26.261937498369832	24.569169489188717
48	25.188028057493643	26.651638084414675	23.933824556827606	24.226509301264077
49	23.903456884425857	25.147227319726877	25.95150487652653	24.997810919320735
50	23.978440735310283	26.46822653995413	23.86647117016579	25.686861554569795
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	17.0
2	22.0
3	66.0
4	110.0
5	143.5
6	177.0
7	165.5
8	154.0
9	213.0
10	272.0
11	385.5
12	499.0
13	851.5
14	1204.0
15	1749.0
16	2294.0
17	2697.0
18	3100.0
19	3507.5
20	3915.0
21	4224.0
22	4533.0
23	4564.5
24	4596.0
25	4273.5
26	3951.0
27	4079.5
28	4208.0
29	4703.5
30	5199.0
31	5892.0
32	6585.0
33	7444.5
34	8304.0
35	9570.5
36	10837.0
37	13168.5
38	15500.0
39	17389.5
40	19279.0
41	21760.5
42	24242.0
43	25687.0
44	27132.0
45	32251.0
46	37370.0
47	41211.0
48	45052.0
49	44681.5
50	44311.0
51	41050.5
52	37790.0
53	36971.5
54	36153.0
55	36663.0
56	37173.0
57	36454.5
58	35736.0
59	32876.5
60	30017.0
61	26773.5
62	23530.0
63	20983.5
64	18437.0
65	15844.5
66	13252.0
67	11519.5
68	9787.0
69	8949.0
70	8111.0
71	6384.0
72	4657.0
73	4165.5
74	3674.0
75	2676.0
76	1678.0
77	1556.0
78	1434.0
79	1208.5
80	983.0
81	781.0
82	579.0
83	503.5
84	428.0
85	348.5
86	269.0
87	196.0
88	123.0
89	86.0
90	49.0
91	35.0
92	21.0
93	14.0
94	7.0
95	5.0
96	3.0
97	3.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013413941183594005
2	0.004471313727864669
3	5.589142159830836E-4
4	3.726094773220557E-4
5	0.0
6	5.589142159830836E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	3.726094773220557E-4
47	1.8630473866102786E-4
48	0.0
49	0.0
50	3.726094773220557E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	536755.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.12118282229623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.18374548879069	45.80190385441683
2	12.06049576787312	13.778195673684207
3	3.3955257967778234	5.818693494467073
4	1.463598163239678	3.344098330431624
5	0.7471244775874396	2.1338316937642348
6	0.41790732572377304	1.4322816453266722
7	0.290928975665628	1.1632745045109791
8	0.21451097712203077	0.9802496593261538
9	0.1497725030506259	0.769966427565695
>10	0.8861030903022984	9.570966215511566
>50	0.104001925776076	4.2606343814390275
>100	0.08136428139361027	8.639520713184
>500	0.004265063137568276	1.551519315946515
>1k	6.561635596258886E-4	0.7548640904254817
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2173	0.40484019711041347	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1855	0.34559529021620666	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	937	0.17456754012538309	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	901	0.16786056953358608	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	844	0.1572411994299075	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	648	0.12072547065234604	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	592	0.11029240528732849	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	584	0.10880196737804025	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	570	0.10619370103678587	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	563	0.10488956786615868	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	553	0.1030265204795484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.8630473866102784E-4	0.0
10	0.0	0.0	0.0	1.8630473866102784E-4	0.0
11	0.0	0.0	0.0	3.7260947732205567E-4	0.0
12	0.0	0.0	0.0	3.7260947732205567E-4	0.0
13	0.0	0.0	0.0	9.315236933051392E-4	0.0
14	0.0	0.0	0.0	9.315236933051392E-4	0.0
15	0.0	0.0	0.0	9.315236933051392E-4	0.0
16	0.0	0.0	0.0	9.315236933051392E-4	0.0
17	0.0	0.0	0.0	0.001117828431966167	0.0
18	0.0	0.0	0.0	0.001117828431966167	0.0
19	0.0	0.0	0.0	0.001117828431966167	0.0
20	0.0	0.0	0.0	0.001117828431966167	0.0
21	0.0	0.0	0.0	0.0016767426479492506	0.0
22	0.0	0.0	0.0	0.0027945710799154176	0.0
23	0.0	0.0	0.0	0.006334361114474947	0.0
24	1.8630473866102784E-4	0.0	0.0	0.01043306536501756	0.0
25	1.8630473866102784E-4	0.0	0.0	0.011923503274305781	0.0
26	1.8630473866102784E-4	0.0	0.0	0.014531769615560172	0.0
27	1.8630473866102784E-4	0.0	0.0	0.018444169127441755	0.0
28	1.8630473866102784E-4	0.0	0.0	0.02775940606049315	0.0
29	3.7260947732205567E-4	0.0	0.0	0.048066622574545186	0.0
30	3.7260947732205567E-4	0.0	0.0	0.0816014755335302	0.0
31	3.7260947732205567E-4	0.0	0.0	0.133953107097279	0.0
32	3.7260947732205567E-4	0.0	0.0	0.2127600115508938	0.0
33	3.7260947732205567E-4	0.0	0.0	0.28355581224208437	0.0
34	3.7260947732205567E-4	0.0	0.0	0.36180380247971605	0.0
35	3.7260947732205567E-4	0.0	0.0	0.4396791832400257	0.0
36	3.7260947732205567E-4	0.0	0.0	0.5659937960522026	0.0
37	3.7260947732205567E-4	0.0	0.0	0.749876573110637	0.0
38	3.7260947732205567E-4	0.0	0.0	0.9576063567176831	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGAC	30	0.005742263	29.333271	23
CGCAATA	190	0.0	25.47363	36
TTAGTGT	45	0.0013972198	24.444391	4
ATACGAA	210	0.0	24.095188	40
CTAGCGG	230	0.0	22.956472	29
AATACGA	225	0.0	22.48884	39
GTGTATA	50	0.002577224	22.002	1
TACGAAT	230	0.0	21.999952	41
TAGCGGC	245	0.0	21.550974	30
TCTAGCG	235	0.0	21.53187	28
CAATACG	225	0.0	21.511065	38
CGAATGC	260	0.0	21.153803	43
GCAATAC	230	0.0	21.043434	37
ATACTGA	85	6.0981984E-6	20.707767	1
GCGCAAT	235	0.0	20.595703	35
AGCGGCG	260	0.0	20.30765	31
GTATAGA	55	0.004478061	20.00182	1
TTTACAC	55	0.0044804765	19.999958	3
TCATATA	55	0.0044804765	19.999958	2
CGCAAAT	125	2.2808308E-8	19.359959	23
>>END_MODULE