Basic Statistics
Measure | Value |
---|---|
Filename | SRR4062686_2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 516445 |
Sequences flagged as poor quality | 0 |
Sequence length | 25 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTATCAACGCAGAGTACTTTTTTTT | 2844 | 0.5506878757660544 | No Hit |
GTACTTTTTTTTTTTTTTTTTTTTT | 2248 | 0.43528352486712046 | No Hit |
TATCAACGCAGAGTACTTTTTTTTT | 1840 | 0.3562818886812729 | No Hit |
GAGTACTTTTTTTTTTTTTTTTTTT | 1682 | 0.325688117805381 | No Hit |
ACGCAGAGTACTTTTTTTTTTTTTT | 1374 | 0.26604962774351576 | No Hit |
GTACATGGAAGCAGTGGTATCAACG | 1086 | 0.21028376690644693 | No Hit |
GGTATCAACGCAGAGTACTTTTTTT | 940 | 0.1820135735654329 | No Hit |
TCCATGTACTCTGCGTTGATACCAC | 840 | 0.1626504274414507 | No Hit |
GTACATGGGGTGGTATCAACGCAAA | 627 | 0.12140692619736855 | No Hit |
GCAGAGTACTTTTTTTTTTTTTTTT | 585 | 0.11327440482529602 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGACCAT | 65 | 9.946234E-9 | 17.543478 | 10 |
AACTATA | 35 | 0.002181718 | 16.269844 | 2 |
CCGACCA | 70 | 4.404974E-7 | 14.932843 | 9 |
GACCATA | 70 | 4.4098306E-7 | 14.931393 | 11 |
ATGGGTA | 50 | 0.0014994254 | 13.297352 | 5 |
TATACTG | 50 | 0.0014994254 | 13.297352 | 5 |
ATCCTAT | 50 | 0.0015137375 | 13.280602 | 1 |
CGGTCCA | 80 | 1.985627E-6 | 13.066237 | 10 |
ACTCTAA | 75 | 1.4697172E-5 | 12.670291 | 10 |
CCATTAA | 60 | 4.1343892E-4 | 12.648192 | 1 |
CGAACCT | 85 | 3.9163388E-6 | 12.297634 | 16 |
TCCGACC | 85 | 3.9163388E-6 | 12.297634 | 8 |
GAACACT | 85 | 3.9163388E-6 | 12.297634 | 6 |
CGCCAGT | 70 | 1.08625514E-4 | 12.216595 | 18 |
GCAGTTA | 55 | 0.0030571055 | 12.094368 | 15 |
CTTACAC | 55 | 0.0030794041 | 12.082641 | 3 |
TCTTACA | 55 | 0.0030861208 | 12.079126 | 2 |
GAATATA | 55 | 0.0030973433 | 12.073275 | 1 |
GCGTTAT | 55 | 0.0030973433 | 12.073275 | 1 |
GCCGGTC | 80 | 2.8493032E-5 | 11.878397 | 8 |