FastQCFastQC Report
Thu 2 Feb 2017
SRR4062686_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062686_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516445
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28440.5506878757660544No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22480.43528352486712046No Hit
TATCAACGCAGAGTACTTTTTTTTT18400.3562818886812729No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16820.325688117805381No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13740.26604962774351576No Hit
GTACATGGAAGCAGTGGTATCAACG10860.21028376690644693No Hit
GGTATCAACGCAGAGTACTTTTTTT9400.1820135735654329No Hit
TCCATGTACTCTGCGTTGATACCAC8400.1626504274414507No Hit
GTACATGGGGTGGTATCAACGCAAA6270.12140692619736855No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5850.11327440482529602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAT659.946234E-917.54347810
AACTATA350.00218171816.2698442
CCGACCA704.404974E-714.9328439
GACCATA704.4098306E-714.93139311
ATGGGTA500.001499425413.2973525
TATACTG500.001499425413.2973525
ATCCTAT500.001513737513.2806021
CGGTCCA801.985627E-613.06623710
ACTCTAA751.4697172E-512.67029110
CCATTAA604.1343892E-412.6481921
CGAACCT853.9163388E-612.29763416
TCCGACC853.9163388E-612.2976348
GAACACT853.9163388E-612.2976346
CGCCAGT701.08625514E-412.21659518
GCAGTTA550.003057105512.09436815
CTTACAC550.003079404112.0826413
TCTTACA550.003086120812.0791262
GAATATA550.003097343312.0732751
GCGTTAT550.003097343312.0732751
GCCGGTC802.8493032E-511.8783978