##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062686_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	516445
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.941653031784604	32.0	32.0	32.0	32.0	32.0
2	30.692120167684845	32.0	32.0	32.0	32.0	32.0
3	30.701890811219005	32.0	32.0	32.0	32.0	32.0
4	30.71085788418902	32.0	32.0	32.0	32.0	32.0
5	30.596030555044585	32.0	32.0	32.0	32.0	32.0
6	34.17521130033208	36.0	36.0	36.0	32.0	36.0
7	34.00455421196836	36.0	36.0	36.0	32.0	36.0
8	33.9817734705535	36.0	36.0	36.0	32.0	36.0
9	34.10882862647523	36.0	36.0	36.0	32.0	36.0
10	33.856745636030944	36.0	36.0	36.0	32.0	36.0
11	34.17639245224564	36.0	36.0	36.0	32.0	36.0
12	33.949665501650706	36.0	36.0	36.0	32.0	36.0
13	34.089860488532175	36.0	36.0	36.0	32.0	36.0
14	33.99544191540242	36.0	36.0	36.0	32.0	36.0
15	33.968265739817404	36.0	36.0	36.0	32.0	36.0
16	33.96341914434257	36.0	36.0	36.0	32.0	36.0
17	33.89180261208841	36.0	36.0	36.0	32.0	36.0
18	33.915679307573896	36.0	36.0	36.0	32.0	36.0
19	33.89640910455131	36.0	36.0	36.0	32.0	36.0
20	33.84389237963384	36.0	36.0	36.0	32.0	36.0
21	33.79433240712951	36.0	36.0	36.0	32.0	36.0
22	33.77684361355033	36.0	36.0	36.0	32.0	36.0
23	33.76610868533919	36.0	36.0	36.0	32.0	36.0
24	33.773762937002004	36.0	36.0	36.0	32.0	36.0
25	33.303914259988964	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	20.0
5	58.0
6	82.0
7	26.0
8	72.0
9	90.0
10	54.0
11	19.0
12	34.0
13	27.0
14	168.0
15	230.0
16	327.0
17	412.0
18	620.0
19	818.0
20	1201.0
21	1547.0
22	2175.0
23	3138.0
24	4041.0
25	5091.0
26	6814.0
27	8657.0
28	11299.0
29	14748.0
30	18688.0
31	25285.0
32	35943.0
33	51444.0
34	113006.0
35	210308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.097671500278864	17.751905558654023	11.077027948193592	25.07339499287352
2	16.35419369580456	21.049808488472515	37.322285435441835	25.27371238028109
3	19.40331166479802	25.15273509586245	28.357896815945256	27.08605642339427
4	12.389589615178293	15.656633144127552	36.14314569619788	35.81063154449628
5	14.087006041494355	37.21494664541135	33.465113711422276	15.232933601672016
6	32.830443070342646	35.683512328172704	17.611232507322516	13.874812094162134
7	29.066815990575545	31.302993195169964	21.297257917008974	18.332932897245517
8	27.947697268971076	32.433197156567104	20.425933534368486	19.193172040093334
9	27.611034878402368	14.33006953973533	19.460660046049725	38.59823553581258
10	15.514677801751295	27.368774074483326	32.47978567288363	24.63676245088175
11	36.218260630192965	22.089515781964245	22.6263080555674	19.065915532275387
12	24.311087931412217	24.693035943828438	29.43655882290215	21.5593173018572
13	29.323684327861987	20.416253908680808	25.917582811691243	24.34247895176596
14	23.262766081191113	20.169826683726882	26.527664689232676	30.03974254584933
15	24.3785800823843	27.666585897746547	24.224085291979648	23.73074872788951
16	24.29512690138852	26.67439466690445	25.29614847065072	23.734329961056304
17	22.520341122120403	26.37531575508843	26.83302735219366	24.271315770597507
18	23.44548535444795	25.603154784523873	28.157498817343562	22.793861043684615
19	24.2129182613821	25.80511317147699	27.325957895634843	22.65601067150607
20	24.677175154166918	24.737853236584424	27.35883622151959	23.22613538772907
21	25.576115490653027	24.78043049195203	26.10308230996817	23.54037170742678
22	24.56906164129872	25.38623983617652	26.628296394836305	23.416402127688457
23	23.4706144688112	25.378395672154035	26.992806313381035	24.158183545653735
24	23.235852403932846	25.97821850903232	27.090171960159687	23.69575712687515
25	23.610397516756272	25.538362509379052	27.09310419037685	23.758135783487823
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	322.0
1	322.0
2	580.5
3	839.0
4	839.0
5	839.0
6	1964.5
7	3090.0
8	3090.0
9	3090.0
10	3091.5
11	3093.0
12	3093.0
13	3093.0
14	2697.5
15	2302.0
16	2302.0
17	2302.0
18	3767.5
19	5233.0
20	5233.0
21	5233.0
22	7755.5
23	10278.0
24	10278.0
25	10278.0
26	14521.0
27	18764.0
28	18764.0
29	18764.0
30	23013.0
31	27262.0
32	27262.0
33	27262.0
34	31384.5
35	35507.0
36	35507.0
37	35507.0
38	39193.5
39	42880.0
40	42880.0
41	42880.0
42	48558.0
43	54236.0
44	54236.0
45	54236.0
46	61915.0
47	69594.0
48	69594.0
49	69594.0
50	68551.5
51	67509.0
52	67509.0
53	67509.0
54	60830.0
55	54151.0
56	54151.0
57	54151.0
58	48723.0
59	43295.0
60	43295.0
61	43295.0
62	37952.5
63	32610.0
64	32610.0
65	32610.0
66	27021.0
67	21432.0
68	21432.0
69	21432.0
70	16289.0
71	11146.0
72	11146.0
73	11146.0
74	8724.0
75	6302.0
76	6302.0
77	6302.0
78	5054.0
79	3806.0
80	3806.0
81	3806.0
82	2667.5
83	1529.0
84	1529.0
85	1529.0
86	1145.0
87	761.0
88	761.0
89	761.0
90	499.5
91	238.0
92	238.0
93	238.0
94	150.5
95	63.0
96	63.0
97	63.0
98	133.0
99	203.0
100	203.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011811519135629157
2	0.0052280494534752
3	0.005421680914715022
4	0.01471599105422649
5	0.03524092594564765
6	0.0443416046239193
7	0.06602832828277938
8	0.08694052609668018
9	0.0931367328563545
10	0.11579161382141372
11	0.11675977112761281
12	0.12934581610820126
13	0.11734066551133228
14	0.1210196632748889
15	0.11036993290669869
16	0.11075719582917833
17	0.11966424304621015
18	0.12721587003456322
19	0.1314757621818393
20	0.11772792843381193
21	0.12837765880200214
22	0.1487089622321835
23	0.13844649478647292
24	0.13283118241051806
25	0.12934581610820126
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	516445.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.0630431243895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.62680287517887	55.06077324946972
2	10.438244875968726	13.582879530161819
3	2.314328215772647	4.517317095204214
4	0.8249329694859142	2.146905974735709
5	0.4227600560218328	1.3753027878108914
6	0.24703992461309046	0.9643901561128462
7	0.16128880065719833	0.7345758134848249
8	0.12390037778453075	0.6449068498338459
9	0.09533715074091385	0.558263263501125
>10	0.6044495091524238	7.895192211912534
>50	0.08448943838797097	3.838091435044212
>100	0.05344031384249446	5.949148363403002
>500	0.0011941969573741715	0.5811816992621452
>1k	0.0017912954360612572	2.1510715700631673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2844	0.5506878757660544	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2248	0.43528352486712046	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1840	0.3562818886812729	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1682	0.325688117805381	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1374	0.26604962774351576	No Hit
GTACATGGAAGCAGTGGTATCAACG	1086	0.21028376690644693	No Hit
GGTATCAACGCAGAGTACTTTTTTT	940	0.1820135735654329	No Hit
TCCATGTACTCTGCGTTGATACCAC	840	0.1626504274414507	No Hit
GTACATGGGGTGGTATCAACGCAAA	627	0.12140692619736855	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	585	0.11327440482529602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9363146123982226E-4
2	0.0	0.0	0.0	0.0	1.9363146123982226E-4
3	0.0	0.0	0.0	0.0	1.9363146123982226E-4
4	0.0	0.0	0.0	0.0	1.9363146123982226E-4
5	0.0	0.0	0.0	0.0	1.9363146123982226E-4
6	0.0	0.0	0.0	0.0	1.9363146123982226E-4
7	0.0	0.0	0.0	0.0	1.9363146123982226E-4
8	0.0	0.0	0.0	0.0	1.9363146123982226E-4
9	0.0	0.0	0.0	0.0	1.9363146123982226E-4
10	0.0	0.0	0.0	0.0	1.9363146123982226E-4
11	0.0	0.0	0.0	1.9363146123982226E-4	1.9363146123982226E-4
12	0.0	0.0	0.0	1.9363146123982226E-4	1.9363146123982226E-4
13	0.0	0.0	0.0	1.9363146123982226E-4	1.9363146123982226E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCAT	65	9.946234E-9	17.543478	10
AACTATA	35	0.002181718	16.269844	2
CCGACCA	70	4.404974E-7	14.932843	9
GACCATA	70	4.4098306E-7	14.931393	11
ATGGGTA	50	0.0014994254	13.297352	5
TATACTG	50	0.0014994254	13.297352	5
ATCCTAT	50	0.0015137375	13.280602	1
CGGTCCA	80	1.985627E-6	13.066237	10
ACTCTAA	75	1.4697172E-5	12.670291	10
CCATTAA	60	4.1343892E-4	12.648192	1
CGAACCT	85	3.9163388E-6	12.297634	16
TCCGACC	85	3.9163388E-6	12.297634	8
GAACACT	85	3.9163388E-6	12.297634	6
CGCCAGT	70	1.08625514E-4	12.216595	18
GCAGTTA	55	0.0030571055	12.094368	15
CTTACAC	55	0.0030794041	12.082641	3
TCTTACA	55	0.0030861208	12.079126	2
GAATATA	55	0.0030973433	12.073275	1
GCGTTAT	55	0.0030973433	12.073275	1
GCCGGTC	80	2.8493032E-5	11.878397	8
>>END_MODULE