FastQCFastQC Report
Thu 2 Feb 2017
SRR4062686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516445
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.1314757621818393No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6540.12663497565084375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGCT551.5919664E-423.99984429
GTAAACG602.8698213E-421.99985527
TATACAG1158.742063E-921.043345
CTAGAAC755.4651384E-520.5331973
GTATAGT550.004480365719.9998686
ATACGAA1101.262215E-719.99986840
GCCGATC901.0001968E-519.55542613
CGAATGC1152.0159496E-719.13030843
TCGCACG708.115079E-418.8570218
ATAGTGC708.115079E-418.857028
CGCTAGA1051.7887505E-618.85701837
GTATAGA951.5903292E-518.5315761
CCGCTAG951.5943151E-518.52619436
GCTTAGA600.007394378518.338541
CGCAATA1203.153491E-718.33321236
CAATACG1203.153491E-718.33321238
TACGAAT1456.2827894E-918.20677841
TTAGAAC851.4288956E-418.1175273
CCGTCGT851.4288956E-418.1175279
TAGGACG851.4288956E-418.1175274
Produced by FastQC (version 0.11.5)