##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062686_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	516445
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.111115414032472	32.0	32.0	32.0	32.0	32.0
2	31.35380921492124	32.0	32.0	32.0	32.0	32.0
3	31.445764795864033	32.0	32.0	32.0	32.0	32.0
4	31.564937214998693	32.0	32.0	32.0	32.0	32.0
5	31.486806920388425	32.0	32.0	32.0	32.0	32.0
6	35.065660428506426	36.0	36.0	36.0	36.0	36.0
7	35.07376003253009	36.0	36.0	36.0	36.0	36.0
8	35.01584486247325	36.0	36.0	36.0	36.0	36.0
9	35.10592028192741	36.0	36.0	36.0	36.0	36.0
10	35.007809156831804	36.0	36.0	36.0	36.0	36.0
11	35.142745113226	36.0	36.0	36.0	36.0	36.0
12	35.049503819380575	36.0	36.0	36.0	36.0	36.0
13	35.0829304185344	36.0	36.0	36.0	36.0	36.0
14	35.04715894238496	36.0	36.0	36.0	36.0	36.0
15	35.01679365663333	36.0	36.0	36.0	36.0	36.0
16	35.026419076571564	36.0	36.0	36.0	36.0	36.0
17	35.00274375780577	36.0	36.0	36.0	36.0	36.0
18	35.011042802234506	36.0	36.0	36.0	36.0	36.0
19	34.982735818915856	36.0	36.0	36.0	36.0	36.0
20	34.94783955697122	36.0	36.0	36.0	36.0	36.0
21	34.93744348381725	36.0	36.0	36.0	32.0	36.0
22	34.9320624655094	36.0	36.0	36.0	32.0	36.0
23	34.90655152049105	36.0	36.0	36.0	32.0	36.0
24	34.896436212955884	36.0	36.0	36.0	32.0	36.0
25	34.87388589297989	36.0	36.0	36.0	32.0	36.0
26	34.822596791526685	36.0	36.0	36.0	32.0	36.0
27	34.809135532341294	36.0	36.0	36.0	32.0	36.0
28	34.78328766858039	36.0	36.0	36.0	32.0	36.0
29	34.76074509386285	36.0	36.0	36.0	32.0	36.0
30	34.738917019237284	36.0	36.0	36.0	32.0	36.0
31	34.736221669296825	36.0	36.0	36.0	32.0	36.0
32	34.68357908392956	36.0	36.0	36.0	32.0	36.0
33	34.62569876753575	36.0	36.0	36.0	32.0	36.0
34	34.59461510906292	36.0	36.0	36.0	32.0	36.0
35	34.54422639390448	36.0	36.0	36.0	32.0	36.0
36	34.513655858803936	36.0	36.0	36.0	32.0	36.0
37	34.48767632564939	36.0	36.0	36.0	32.0	36.0
38	34.43952018123905	36.0	36.0	36.0	32.0	36.0
39	34.4143209828733	36.0	36.0	36.0	32.0	36.0
40	34.3888022925965	36.0	36.0	36.0	32.0	36.0
41	34.34578706348208	36.0	36.0	36.0	32.0	36.0
42	34.30449128174346	36.0	36.0	36.0	32.0	36.0
43	34.283768842761575	36.0	36.0	36.0	32.0	36.0
44	34.22249997579607	36.0	36.0	36.0	32.0	36.0
45	34.14996950304486	36.0	36.0	36.0	32.0	36.0
46	34.140723600770656	36.0	36.0	36.0	32.0	36.0
47	34.06582501524848	36.0	36.0	36.0	32.0	36.0
48	33.9986097261083	36.0	36.0	36.0	32.0	36.0
49	33.968110834648414	36.0	36.0	36.0	32.0	36.0
50	33.39762801459981	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	12.0
21	32.0
22	60.0
23	179.0
24	434.0
25	1075.0
26	2179.0
27	4005.0
28	6828.0
29	10235.0
30	14136.0
31	19450.0
32	27611.0
33	43251.0
34	88475.0
35	298480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.3246320902156	18.463561486783522	11.513412076388123	25.69839434661275
2	15.807787711379492	21.381448369475763	37.03819886179901	25.772565057345737
3	18.87108111091102	25.32932125838189	28.72816062241379	27.071437008293298
4	12.322730053984555	16.15160598244921	36.088327098388206	35.437336865178025
5	13.70504119509338	37.36525670690974	33.524189410295385	15.405512687701497
6	33.02300544300145	35.87540154479778	17.131609877566355	13.969983134634411
7	28.90801537433802	31.192092091122962	21.31456399035715	18.58532854418186
8	27.937534490604033	32.66098035608826	20.283670090716342	19.11781506259137
9	27.64592550997686	14.000522804945348	19.2171480022074	39.136403682870395
10	15.56564590614683	27.335921540531906	32.36511148331381	24.733321070007456
11	36.3177105016023	21.83911161885583	22.52030709949753	19.32287078004434
12	24.239948106768388	24.612495038193806	29.46528671978623	21.682270135251574
13	29.549516405425553	20.156066957759297	25.957846430888093	24.336570205927057
14	23.101588746139473	20.07687169011221	26.52421845501457	30.297321108733748
15	24.567766170647406	27.520258691632215	23.989776258846536	23.92219887887384
16	24.17546883017553	26.56797916525477	25.29582046490914	23.960731539660564
17	22.622157248109673	26.38112480515834	26.80692038842471	24.189797558307273
18	23.469875785417614	25.28691341769211	28.282973017455877	22.960237779434404
19	24.453136345593432	25.576973346629362	27.239880335756954	22.730009972020255
20	24.8132908634995	24.481212907473207	27.347152165283816	23.358344063743477
21	25.745626349369243	24.637279865232504	26.09764834590324	23.51944543949501
22	24.613075932577523	25.230373031010078	26.71184734095596	23.444703695456436
23	23.315551510809478	25.06249455411515	27.174626533319135	24.44732740175624
24	23.459226055049424	25.829662403547328	27.094850371288327	23.61626117011492
25	23.783558752626128	25.158729390351343	27.235233180687196	23.822478676335333
26	22.980181819942107	25.86083706880694	27.661609658337287	23.497371452913672
27	23.89102421361423	25.707480951505	27.011395211493966	23.39009962338681
28	23.153481977751746	25.47686588116837	27.654251662810175	23.71540047826971
29	23.055310826903156	25.879619320547203	27.412599599182876	23.65247025336677
30	23.25804296682125	25.934223392616833	27.482500556690454	23.325233083871467
31	23.30122278267773	25.642227149067182	26.903736119044623	24.152813949210465
32	23.057440772976793	25.85909438565578	27.05767313073028	24.025791710637144
33	23.06460513704267	25.41432291918791	27.41434228233403	24.106729661435388
34	23.32484582094899	25.80836294281095	27.607199217728894	23.259592018511167
35	24.0970480883734	25.60834164335021	27.214320982873296	23.08028928540309
36	23.206536998131455	26.01303139734144	27.052638712738048	23.727792891789058
37	23.66079640620008	26.0138059231864	26.926971894393404	23.39842577622012
38	23.09616706522476	25.899950623977386	27.09775484320692	23.906127467590935
39	23.374996369410102	25.636611836691227	27.0164296294862	23.971962164412474
40	23.751803192982795	25.750273504439004	27.283834677458398	23.214088625119807
41	22.77377068226045	25.953199275818335	27.773141380011424	23.499888661909786
42	23.657117408436523	26.177230876472805	27.231941445846118	22.933710269244546
43	22.846963374609107	25.504167917203187	27.401756237353446	24.24711247083426
44	22.402191908141234	25.96636621518264	27.379488619310866	24.25195325736526
45	22.568134070423763	26.086030458228855	27.15448886135019	24.19134660999719
46	22.394102748797547	25.70055998327021	27.36114661028108	24.544190657651168
47	22.654106439214246	25.799455895593915	27.48153239938425	24.06490526580759
48	22.801653612678987	26.667505736332036	26.607286351886454	23.923554299102516
49	22.16751057711857	26.477940535778256	27.181984528846247	24.172564358256928
50	22.092381570157517	26.944205094443745	26.53196371346416	24.431449621934572
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	102.0
1	85.5
2	69.0
3	210.5
4	352.0
5	369.5
6	387.0
7	343.0
8	299.0
9	389.5
10	480.0
11	709.5
12	939.0
13	1461.0
14	1983.0
15	2552.0
16	3121.0
17	3457.5
18	3794.0
19	3966.5
20	4139.0
21	4459.5
22	4780.0
23	5046.5
24	5313.0
25	5485.5
26	5658.0
27	6433.5
28	7209.0
29	7998.5
30	8788.0
31	10040.5
32	11293.0
33	12834.5
34	14376.0
35	15990.0
36	17604.0
37	19548.5
38	21493.0
39	22841.0
40	24189.0
41	26041.5
42	27894.0
43	28874.0
44	29854.0
45	32108.5
46	34363.0
47	35905.0
48	37447.0
49	37331.5
50	37216.0
51	35434.5
52	33653.0
53	32656.0
54	31659.0
55	31481.5
56	31304.0
57	29859.5
58	28415.0
59	26109.0
60	23803.0
61	21062.5
62	18322.0
63	15824.0
64	13326.0
65	11470.0
66	9614.0
67	8414.5
68	7215.0
69	6465.0
70	5715.0
71	4575.5
72	3436.0
73	3016.5
74	2597.0
75	1976.0
76	1355.0
77	1209.0
78	1063.0
79	923.0
80	783.0
81	608.5
82	434.0
83	374.5
84	315.0
85	243.0
86	171.0
87	128.5
88	86.0
89	55.0
90	24.0
91	15.5
92	7.0
93	6.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015877779821665425
2	0.0030981033798371557
3	7.745258449592889E-4
4	1.9363146123982223E-4
5	0.0
6	3.8726292247964446E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.9363146123982223E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	516445.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.42627848103817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.77630355818286	60.40905841462651
2	9.893236168692644	13.934876109900543
3	2.0462148789615875	4.323218966933777
4	0.743753313381974	2.09519111877735
5	0.3603936184348436	1.2690590667340649
6	0.23394266875215597	0.9885426922881959
7	0.13426452582458248	0.661902562009261
8	0.10057509706089213	0.5666503835094265
9	0.08612807335426795	0.5459111711174661
>10	0.5199381344378745	7.202766724451654
>50	0.06823291847995161	3.363773270614781
>100	0.03646455123582839	4.379714102434903
>500	5.524932005428165E-4	0.2593354166020559
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	679	0.1314757621818393	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	654	0.12663497565084375	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.9363146123982226E-4	0.0
12	0.0	0.0	0.0	1.9363146123982226E-4	0.0
13	0.0	0.0	0.0	1.9363146123982226E-4	0.0
14	0.0	0.0	0.0	1.9363146123982226E-4	0.0
15	0.0	0.0	0.0	1.9363146123982226E-4	0.0
16	0.0	0.0	0.0	1.9363146123982226E-4	0.0
17	0.0	0.0	0.0	1.9363146123982226E-4	0.0
18	0.0	0.0	0.0	1.9363146123982226E-4	1.9363146123982226E-4
19	0.0	0.0	0.0	1.9363146123982226E-4	1.9363146123982226E-4
20	0.0	0.0	0.0	7.74525844959289E-4	1.9363146123982226E-4
21	0.0	0.0	0.0	0.0011617887674389336	1.9363146123982226E-4
22	0.0	0.0	0.0	0.0029044719185973337	1.9363146123982226E-4
23	0.0	0.0	0.0	0.004840786530995556	1.9363146123982226E-4
24	1.9363146123982226E-4	0.0	0.0	0.009681573061991113	1.9363146123982226E-4
25	1.9363146123982226E-4	0.0	0.0	0.0121987820581088	1.9363146123982226E-4
26	1.9363146123982226E-4	0.0	0.0	0.016845937127864537	5.808943837194668E-4
27	1.9363146123982226E-4	0.0	0.0	0.02246124950381938	7.74525844959289E-4
28	1.9363146123982226E-4	0.0	0.0	0.0400817124766432	7.74525844959289E-4
29	1.9363146123982226E-4	0.0	0.0	0.07435448111609175	7.74525844959289E-4
30	1.9363146123982226E-4	0.0	0.0	0.14193186108878972	7.74525844959289E-4
31	1.9363146123982226E-4	0.0	0.0	0.2749566749605476	7.74525844959289E-4
32	1.9363146123982226E-4	0.0	0.0	0.42966821249116555	7.74525844959289E-4
33	1.9363146123982226E-4	0.0	0.0	0.5830243297931048	7.74525844959289E-4
34	1.9363146123982226E-4	0.0	0.0	0.761552537056221	7.74525844959289E-4
35	1.9363146123982226E-4	0.0	0.0	0.9590566275208395	7.74525844959289E-4
36	1.9363146123982226E-4	0.0	0.0	1.2301406732565907	7.74525844959289E-4
37	1.9363146123982226E-4	0.0	0.0	1.6247615912633484	7.74525844959289E-4
38	1.9363146123982226E-4	0.0	0.0	2.114261925277619	7.74525844959289E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGCT	55	1.5919664E-4	23.999844	29
GTAAACG	60	2.8698213E-4	21.999855	27
TATACAG	115	8.742063E-9	21.04334	5
CTAGAAC	75	5.4651384E-5	20.533197	3
GTATAGT	55	0.0044803657	19.999868	6
ATACGAA	110	1.262215E-7	19.999868	40
GCCGATC	90	1.0001968E-5	19.555426	13
CGAATGC	115	2.0159496E-7	19.130308	43
TCGCACG	70	8.115079E-4	18.85702	18
ATAGTGC	70	8.115079E-4	18.85702	8
CGCTAGA	105	1.7887505E-6	18.857018	37
GTATAGA	95	1.5903292E-5	18.531576	1
CCGCTAG	95	1.5943151E-5	18.526194	36
GCTTAGA	60	0.0073943785	18.33854	1
CGCAATA	120	3.153491E-7	18.333212	36
CAATACG	120	3.153491E-7	18.333212	38
TACGAAT	145	6.2827894E-9	18.206778	41
TTAGAAC	85	1.4288956E-4	18.117527	3
CCGTCGT	85	1.4288956E-4	18.117527	9
TAGGACG	85	1.4288956E-4	18.117527	4
>>END_MODULE