FastQCFastQC Report
Thu 2 Feb 2017
SRR4062685_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062685_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200405
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG270692.2549889412323343No Hit
TCCATGTACTCTGCGTTGATACCAC233431.944593699626376No Hit
GTATCAACGCAGAGTACTTTTTTTT139851.1650234712451215No Hit
GAGTACATGGAAGCAGTGGTATCAA113250.9434315918377548No Hit
CATGTACTCTGCGTTGATACCACTG95510.7956481354209621No Hit
TATCAACGCAGAGTACTTTTTTTTT90910.7573277352226957No Hit
GTACTTTTTTTTTTTTTTTTTTTTT83540.6959317896876471No Hit
CCCATGTACTCTGCGTTGATACCAC71570.5962154439543321No Hit
GCTTCCATGTACTCTGCGTTGATAC71390.5947159500335303No Hit
GTACATGGGAAGCAGTGGTATCAAC66290.552230288944148No Hit
GCGTTGATACCACTGCTTCCATGTA65850.548564859359966No Hit
CATGGAAGCAGTGGTATCAACGCAG63990.5330700888450148No Hit
GTATCAACGCAGAGTACATGGAAGC61160.5094947122012987No Hit
GAGTACTTTTTTTTTTTTTTTTTTT57100.4756727937654375No Hit
GTACATGGGGTGGTATCAACGCAAA56970.4745898259337473No Hit
GGTATCAACGCAGAGTACTTTTTTT54230.4517641962504321No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52380.4363527309533033No Hit
GTATCAACGCAGAGTACATGGGAAG44330.36929203060633703No Hit
ACTCTGCGTTGATACCACTGCTTCC44240.36854228364593616No Hit
ACGCAGAGTACATGGAAGCAGTGGT42750.35612980619041074No Hit
CAGTGGTATCAACGCAGAGTACATG40060.3337207025962071No Hit
GTCCTACAGTGGACATTTCTAAATT38640.3218913616654379No Hit
GTCCTAAAGTGTGTATTTCTCATTT36530.3043139607049287No Hit
TATCAACGCAGAGTACATGGAAGCA36390.3031476876554163No Hit
CCATAGGGTCTTCTCGTCTTATTAT36360.30289777200194934No Hit
GTACATGGGTGGTATCAACGCAAAA35650.29698310153656476No Hit
CTGTAGGACGTGGAATATGGCAAGA35350.29448394500189523No Hit
CTTTAGGACGTGAAATATGGCGAGG32150.2678262752987533No Hit
GTATCAACGCAGAGTACATGGGGTG29760.24791632823921925No Hit
GTCCTACAGTGTGCATTTCTCATTT28320.23592037687280543No Hit
GTACATGGGAGTGGTATCAACGCAA27020.22509069855590405No Hit
GTATTAGAGGCACTGCCTGCCCAGT25640.21359457849642413No Hit
ATACCACTGCTTCCATGTACTCTGC24550.2045143097537914No Hit
GGTATCAACGCAGAGTACATGGAAG24330.20268159496170043No Hit
TATCAACGCAGAGTACATGGGAAGC23940.19943269146663No Hit
GGTATCAACGCAGAGTACATGGGAA23750.19784989232800596No Hit
GCGTTGATACCACTGCTTCCCATGT23610.1966836192784935No Hit
GAGTACATGGGAAGCAGTGGTATCA22280.18560402530812517No Hit
GATATACACTGTTCTACAAATCCCG21250.1770235878724264No Hit
GCAGAGTACTTTTTTTTTTTTTTTT21210.17669036700113713No Hit
CTCTAATACTTGTAATGCTAGAGGT21060.17544078873380234No Hit
CCATTGGGATGTCCTGATCCAACAT20980.1747743469912238No Hit
CTGTAGGACCTGGAATATGGCGAGA20900.17410790524864525No Hit
AAGCAGTGGTATCAACGCAGAGTAC20800.17327485307042206No Hit
GTCAGGATACCGCGGCCGTTAAACT20660.17210858002090962No Hit
TATCAACGCAGAGTACATGGGGTGG20130.16769340347632675No Hit
GTGGTATCAACGCAGAGTACATGGA19570.1630283112782769No Hit
GTACTCTGCGTTGATACCACTGCTT19570.1630283112782769No Hit
TTGTAGAACAGTGTATATCAATGAG19510.162528479971343No Hit
CTGTTAGTATGAGTAACAAGAATTC18930.15769677733764853No Hit
ATCTATAACTTTATAGATGCAACAC18490.15403134775346655No Hit
CTTCTACACCATTGGGATGTCCTGA17990.14986608686235062No Hit
ACATGGAAGCAGTGGTATCAACGCA17530.146034046842524No Hit
ACGCAGAGTACATGGGAAGCAGTGG17380.1447844685751892No Hit
GCTTCCCATGTACTCTGCGTTGATA17300.14411802683261066No Hit
ATCCTGACCGTGCAAAGGTAGCATA17200.14328497465438747No Hit
GTTATATAATTTAAGCTCCATAGGG16860.14045259724842865No Hit
GAAGCAGTGGTATCAACGCAGAGTA16470.13720369375335822No Hit
CCACTGCTTCCATGTACTCTGCGTT16350.13620403113949042No Hit
GATATATTTTGATCAACGGACCAAG16280.1356208946147342No Hit
GTATCAACGCAGAGTACATGGGAGA16230.1352043685256226No Hit
ATCAACGCAGAGTACTTTTTTTTTT16200.13495445287215563No Hit
GTATCAACGCAGAGTACATGGGAGT16200.13495445287215563No Hit
GTATCCTGACCGTGCAAAGGTAGCA16060.13378817982264318No Hit
AAAAAGTACTCTGCGTTGATACCAC15660.13045597110975046No Hit
CTGAAGGACCTGGAATATGGCGAGA15130.1260407945651676No Hit
GTGTATATCAATGAGTTACAATGAA14990.12487452151565513No Hit
GTTATAGATTAACCCAATTTTAAGT14980.12479121629783282No Hit
TGGAAGCAGTGGTATCAACGCAGAG14570.12137570236711777No Hit
GGTATCAACGCAGAGTACATGGGGT14310.11920976670373748No Hit
CCTCTAGCATTACAAGTATTAGAGG14110.11754366234729113No Hit
CTGCTTCCATGTACTCTGCGTTGAT13950.11621077886213402No Hit
GTCCTTCAGTGTGCATTTCTCATTT13800.11496120059479925No Hit
GTTCATGCTAGTCCCTAATTAAGGA13790.11487789537697693No Hit
GCCTAAAGGAAAGATCCAAAAAGAT13740.11446136928786534No Hit
CCTATAACTTCTCTGTTAACCCAAC13720.11429475885222072No Hit
GATTAAAGATAAGAGACAGTTGGAC13670.11387823276310911No Hit
CTATAGAACTAGTACCGCAAGGGAA13650.11371162232746447No Hit
GTACATGGGAGAAATCGTAAATAGA13560.1129618753670636No Hit
ACCTATAACTTCTCTGTTAACCCAA13470.11221212840666274No Hit
GGTATCAACGCAGAGTACATGGGAG13260.11046271883239406No Hit
ATCGTAAATAGATAGAAACCGACCT13220.1101294979611048No Hit
ATAAATAATCCACCTATAACTTCTC13170.10971297187199319No Hit
TGCTAGAGGTGATGTTTTTGGTAAA12970.10804686751554685No Hit
GTTCTACAGTGTGGTTTTTATCATT12960.10796356229772451No Hit
CCATGTACTCTGCGTTGATACCACT12890.10738042577296829No Hit
GATACCACTGCTTCCATGTACTCTG12830.10688059446603437No Hit
GATTAAACCTTGTACCTTTTGCATA12810.10671398403038973No Hit
ACGCAGAGTACATGGGGTGGTATCA12640.10529779532741033No Hit
GTTGATACCACTGCTTCCATGTACT12480.10396491184225323No Hit
GGTCAGGATACCGCGGCCGTTAAAC12400.1032984700996747No Hit
CCCCATGTACTCTGCGTTGATACCA12310.10254872313927384No Hit
GTGTATATCAATGAGTTACAATGAG12280.10229880748580689No Hit
CACTATAAATAATCCACCTATAACT12250.10204889183233992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCT250.006019061419.00667817
TTGTGCG307.7182427E-419.0011277
AGGGGTC307.729078E-418.9971625
CTAAGGC402.7680013E-416.6190533
TATAGGG402.769763E-416.6176662
TTGCTAA1550.016.54936610
GGGGTCC350.002168950416.286686
CTATCGG350.002170159716.28532214
AGCGCCA350.002171974916.2832835
ATAAGGT350.002171974916.2832835
TGCTAAA1550.015.93576211
GGTCGGT508.709984E-515.20026611
CCAACGA1900.015.00464519
CTTGCTA1650.014.9705849
AACTTGC1600.014.844637
GCTAAAA1750.014.65862412
TCCAACG2600.014.61991118
TAACTTT3000.014.567536
AAACTTG1700.014.53027256
TAGGACC8450.014.2747854
Produced by FastQC (version 0.11.5)