##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062685_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1200405
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.424031889237384	32.0	32.0	32.0	32.0	32.0
2	30.41516738100891	32.0	32.0	32.0	32.0	32.0
3	30.5021996742766	32.0	32.0	32.0	32.0	32.0
4	30.36746181497078	32.0	32.0	32.0	27.0	32.0
5	30.480177939945268	32.0	32.0	32.0	32.0	32.0
6	33.92417142547723	36.0	36.0	36.0	32.0	36.0
7	33.57965186749472	36.0	36.0	36.0	21.0	36.0
8	33.49081101794811	36.0	36.0	36.0	21.0	36.0
9	33.472215627225815	36.0	36.0	36.0	21.0	36.0
10	33.446416001266236	36.0	36.0	36.0	21.0	36.0
11	33.81282150607503	36.0	36.0	36.0	32.0	36.0
12	33.596837733931466	36.0	36.0	36.0	27.0	36.0
13	33.68270375414964	36.0	36.0	36.0	27.0	36.0
14	33.60357629300111	36.0	36.0	36.0	27.0	36.0
15	33.689129918652455	36.0	36.0	36.0	27.0	36.0
16	33.62174516100816	36.0	36.0	36.0	27.0	36.0
17	33.550361752908394	36.0	36.0	36.0	27.0	36.0
18	33.65562372699214	36.0	36.0	36.0	27.0	36.0
19	33.54837575651551	36.0	36.0	36.0	27.0	36.0
20	33.35931706382429	36.0	36.0	36.0	21.0	36.0
21	33.20407945651676	36.0	36.0	36.0	21.0	36.0
22	33.22034146808785	36.0	36.0	36.0	21.0	36.0
23	33.229614171883654	36.0	36.0	36.0	21.0	36.0
24	33.223518729095595	36.0	36.0	36.0	21.0	36.0
25	32.64544133021772	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	13.0
4	55.0
5	122.0
6	201.0
7	46.0
8	206.0
9	220.0
10	145.0
11	51.0
12	86.0
13	61.0
14	1143.0
15	1079.0
16	1348.0
17	1894.0
18	2754.0
19	3861.0
20	5511.0
21	7426.0
22	9730.0
23	12172.0
24	14306.0
25	16948.0
26	20479.0
27	23498.0
28	28883.0
29	36342.0
30	44453.0
31	59374.0
32	81125.0
33	111535.0
34	236097.0
35	479240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00691493793219	20.517703907356495	14.1173040073315	21.358077147379824
2	10.869592382996201	23.434916154809322	43.709298906084555	21.986192556109923
3	18.54558147749501	31.075864160299112	30.161302147029716	20.217252215176167
4	9.86937373043897	20.06135552041379	39.020078019159854	31.049192729987386
5	9.26527795833875	42.2628512188699	35.44906347190416	13.022807350887195
6	26.538562191733327	39.10425768468497	20.839952194183624	13.517227929398077
7	23.900841713370628	36.32138662211763	22.640397406752548	17.137374257759195
8	26.092100754012492	36.18326097270363	21.61321819001982	16.111420083264058
9	26.831660133138868	17.75910420205487	21.30547140216094	34.10376426264531
10	15.410868928795729	29.54045968210756	34.21576550991318	20.832905879183524
11	30.151952917782943	25.32112127207396	25.124695143311836	19.402230666831258
12	23.517316053428008	28.62729526548835	31.462780245117507	16.39260843596614
13	29.21854291378875	24.4672945058373	27.38415266317299	18.930009917200962
14	22.455400486449154	25.114939460768777	27.88766627853051	24.54199377425156
15	23.11128458669442	31.15763259205882	29.60560993347879	16.12547288776797
16	20.32103044668369	29.62884503433655	31.72531763599091	18.324806882988856
17	17.899279591426495	29.947627122461117	32.28751617103302	19.86557711507937
18	19.595721821645807	26.77473332287868	37.027163196054815	16.6023816594207
19	20.38854469665435	32.03871265894271	30.08122417689303	17.491518467509913
20	20.53960545927853	27.539112512751135	35.514753781133415	16.406528246836928
21	21.14764286101576	29.06119010180972	27.18432105304866	22.606845984125858
22	19.289169000700003	32.77541801932137	28.704563525337296	19.230849454641326
23	18.217379878783834	32.14180539507878	29.57891355944222	20.06190116669517
24	18.89327304881711	33.55333844973139	30.024191664720213	17.52919683673129
25	21.085388101571827	30.122781421708595	29.866524524168813	18.925305952550765
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1321.0
1	1321.0
2	2232.0
3	3143.0
4	3143.0
5	3143.0
6	7855.0
7	12567.0
8	12567.0
9	12567.0
10	11995.0
11	11423.0
12	11423.0
13	11423.0
14	10197.0
15	8971.0
16	8971.0
17	8971.0
18	14804.5
19	20638.0
20	20638.0
21	20638.0
22	32465.5
23	44293.0
24	44293.0
25	44293.0
26	67076.5
27	89860.0
28	89860.0
29	89860.0
30	109858.5
31	129857.0
32	129857.0
33	129857.0
34	130033.0
35	130209.0
36	130209.0
37	130209.0
38	124830.5
39	119452.0
40	119452.0
41	119452.0
42	128117.5
43	136783.0
44	136783.0
45	136783.0
46	200382.5
47	263982.0
48	263982.0
49	263982.0
50	199532.0
51	135082.0
52	135082.0
53	135082.0
54	90540.0
55	45998.0
56	45998.0
57	45998.0
58	33156.5
59	20315.0
60	20315.0
61	20315.0
62	15572.5
63	10830.0
64	10830.0
65	10830.0
66	8770.5
67	6711.0
68	6711.0
69	6711.0
70	5307.5
71	3904.0
72	3904.0
73	3904.0
74	3256.5
75	2609.0
76	2609.0
77	2609.0
78	1885.5
79	1162.0
80	1162.0
81	1162.0
82	774.0
83	386.0
84	386.0
85	386.0
86	291.5
87	197.0
88	197.0
89	197.0
90	128.0
91	59.0
92	59.0
93	59.0
94	53.5
95	48.0
96	48.0
97	48.0
98	326.5
99	605.0
100	605.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008747047871343422
2	0.0031655982772480955
3	0.004081955673293597
4	0.015578075732773523
5	0.03673760105964237
6	0.045901175020097384
7	0.06897672035687955
8	0.09063607699068231
9	0.10046609269371587
10	0.12045934497107226
11	0.1233750275948534
12	0.13603742070384578
13	0.12312511194138645
14	0.1260407945651676
15	0.11571094755520012
16	0.11587755799084476
17	0.12512443716912208
18	0.13137232850579597
19	0.13528767374344491
20	0.12429138499089891
21	0.13328834851570928
22	0.1529483799217764
23	0.14328497465438747
24	0.1370370833177136
25	0.13395479025828783
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1200405.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.803142347195344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28096914683312	14.115871786607755
2	12.958429524231132	5.132352489288959
3	5.075323400491605	3.015220552739603
4	2.6486228766709674	2.0980422340301277
5	1.5456455073564832	1.5304319000241702
6	1.02019525360564	1.2121843097451326
7	0.7445136465863493	1.0320596805945275
8	0.5356128038584319	0.8485453278231158
9	0.4259358242224381	0.7591380982062224
>10	2.868887206908491	11.15880967214113
>50	0.39759816851254876	5.605901981076959
>100	0.38871565604777514	16.351817839721257
>500	0.059639725247808806	8.008299771546545
>1k	0.04272065425552262	15.326311191640634
>5k	0.0054986980724930105	7.4623345398068155
>10k+	0.0016919070992286185	6.342678625007034
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	27069	2.2549889412323343	No Hit
TCCATGTACTCTGCGTTGATACCAC	23343	1.944593699626376	No Hit
GTATCAACGCAGAGTACTTTTTTTT	13985	1.1650234712451215	No Hit
GAGTACATGGAAGCAGTGGTATCAA	11325	0.9434315918377548	No Hit
CATGTACTCTGCGTTGATACCACTG	9551	0.7956481354209621	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9091	0.7573277352226957	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	8354	0.6959317896876471	No Hit
CCCATGTACTCTGCGTTGATACCAC	7157	0.5962154439543321	No Hit
GCTTCCATGTACTCTGCGTTGATAC	7139	0.5947159500335303	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6629	0.552230288944148	No Hit
GCGTTGATACCACTGCTTCCATGTA	6585	0.548564859359966	No Hit
CATGGAAGCAGTGGTATCAACGCAG	6399	0.5330700888450148	No Hit
GTATCAACGCAGAGTACATGGAAGC	6116	0.5094947122012987	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	5710	0.4756727937654375	No Hit
GTACATGGGGTGGTATCAACGCAAA	5697	0.4745898259337473	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5423	0.4517641962504321	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5238	0.4363527309533033	No Hit
GTATCAACGCAGAGTACATGGGAAG	4433	0.36929203060633703	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4424	0.36854228364593616	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	4275	0.35612980619041074	No Hit
CAGTGGTATCAACGCAGAGTACATG	4006	0.3337207025962071	No Hit
GTCCTACAGTGGACATTTCTAAATT	3864	0.3218913616654379	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3653	0.3043139607049287	No Hit
TATCAACGCAGAGTACATGGAAGCA	3639	0.3031476876554163	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	3636	0.30289777200194934	No Hit
GTACATGGGTGGTATCAACGCAAAA	3565	0.29698310153656476	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3535	0.29448394500189523	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3215	0.2678262752987533	No Hit
GTATCAACGCAGAGTACATGGGGTG	2976	0.24791632823921925	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2832	0.23592037687280543	No Hit
GTACATGGGAGTGGTATCAACGCAA	2702	0.22509069855590405	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	2564	0.21359457849642413	No Hit
ATACCACTGCTTCCATGTACTCTGC	2455	0.2045143097537914	No Hit
GGTATCAACGCAGAGTACATGGAAG	2433	0.20268159496170043	No Hit
TATCAACGCAGAGTACATGGGAAGC	2394	0.19943269146663	No Hit
GGTATCAACGCAGAGTACATGGGAA	2375	0.19784989232800596	No Hit
GCGTTGATACCACTGCTTCCCATGT	2361	0.1966836192784935	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2228	0.18560402530812517	No Hit
GATATACACTGTTCTACAAATCCCG	2125	0.1770235878724264	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2121	0.17669036700113713	No Hit
CTCTAATACTTGTAATGCTAGAGGT	2106	0.17544078873380234	No Hit
CCATTGGGATGTCCTGATCCAACAT	2098	0.1747743469912238	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2090	0.17410790524864525	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2080	0.17327485307042206	No Hit
GTCAGGATACCGCGGCCGTTAAACT	2066	0.17210858002090962	No Hit
TATCAACGCAGAGTACATGGGGTGG	2013	0.16769340347632675	No Hit
GTGGTATCAACGCAGAGTACATGGA	1957	0.1630283112782769	No Hit
GTACTCTGCGTTGATACCACTGCTT	1957	0.1630283112782769	No Hit
TTGTAGAACAGTGTATATCAATGAG	1951	0.162528479971343	No Hit
CTGTTAGTATGAGTAACAAGAATTC	1893	0.15769677733764853	No Hit
ATCTATAACTTTATAGATGCAACAC	1849	0.15403134775346655	No Hit
CTTCTACACCATTGGGATGTCCTGA	1799	0.14986608686235062	No Hit
ACATGGAAGCAGTGGTATCAACGCA	1753	0.146034046842524	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1738	0.1447844685751892	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1730	0.14411802683261066	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	1720	0.14328497465438747	No Hit
GTTATATAATTTAAGCTCCATAGGG	1686	0.14045259724842865	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1647	0.13720369375335822	No Hit
CCACTGCTTCCATGTACTCTGCGTT	1635	0.13620403113949042	No Hit
GATATATTTTGATCAACGGACCAAG	1628	0.1356208946147342	No Hit
GTATCAACGCAGAGTACATGGGAGA	1623	0.1352043685256226	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1620	0.13495445287215563	No Hit
GTATCAACGCAGAGTACATGGGAGT	1620	0.13495445287215563	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	1606	0.13378817982264318	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1566	0.13045597110975046	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1513	0.1260407945651676	No Hit
GTGTATATCAATGAGTTACAATGAA	1499	0.12487452151565513	No Hit
GTTATAGATTAACCCAATTTTAAGT	1498	0.12479121629783282	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	1457	0.12137570236711777	No Hit
GGTATCAACGCAGAGTACATGGGGT	1431	0.11920976670373748	No Hit
CCTCTAGCATTACAAGTATTAGAGG	1411	0.11754366234729113	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	1395	0.11621077886213402	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1380	0.11496120059479925	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	1379	0.11487789537697693	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	1374	0.11446136928786534	No Hit
CCTATAACTTCTCTGTTAACCCAAC	1372	0.11429475885222072	No Hit
GATTAAAGATAAGAGACAGTTGGAC	1367	0.11387823276310911	No Hit
CTATAGAACTAGTACCGCAAGGGAA	1365	0.11371162232746447	No Hit
GTACATGGGAGAAATCGTAAATAGA	1356	0.1129618753670636	No Hit
ACCTATAACTTCTCTGTTAACCCAA	1347	0.11221212840666274	No Hit
GGTATCAACGCAGAGTACATGGGAG	1326	0.11046271883239406	No Hit
ATCGTAAATAGATAGAAACCGACCT	1322	0.1101294979611048	No Hit
ATAAATAATCCACCTATAACTTCTC	1317	0.10971297187199319	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	1297	0.10804686751554685	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1296	0.10796356229772451	No Hit
CCATGTACTCTGCGTTGATACCACT	1289	0.10738042577296829	No Hit
GATACCACTGCTTCCATGTACTCTG	1283	0.10688059446603437	No Hit
GATTAAACCTTGTACCTTTTGCATA	1281	0.10671398403038973	No Hit
ACGCAGAGTACATGGGGTGGTATCA	1264	0.10529779532741033	No Hit
GTTGATACCACTGCTTCCATGTACT	1248	0.10396491184225323	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	1240	0.1032984700996747	No Hit
CCCCATGTACTCTGCGTTGATACCA	1231	0.10254872313927384	No Hit
GTGTATATCAATGAGTTACAATGAG	1228	0.10229880748580689	No Hit
CACTATAAATAATCCACCTATAACT	1225	0.10204889183233992	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.33052178223183E-5	0.0
3	0.0	0.0	0.0	8.33052178223183E-5	0.0
4	0.0	0.0	0.0	1.666104356446366E-4	0.0
5	0.0	0.0	0.0	1.666104356446366E-4	0.0
6	0.0	0.0	0.0	1.666104356446366E-4	0.0
7	0.0	0.0	0.0	5.831365247562281E-4	0.0
8	0.0	0.0	0.0	7.497469604008647E-4	0.0
9	0.0	0.0	0.0	9.996626138678195E-4	0.0
10	0.0	0.0	0.0	0.001082967831690138	0.0
11	0.0	0.0	0.0	0.001082967831690138	0.0
12	0.0	0.0	0.0	0.001082967831690138	0.0
13	0.0	0.0	0.0	0.0012495782673347744	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTCT	25	0.0060190614	19.006678	17
TTGTGCG	30	7.7182427E-4	19.001127	7
AGGGGTC	30	7.729078E-4	18.997162	5
CTAAGGC	40	2.7680013E-4	16.619053	3
TATAGGG	40	2.769763E-4	16.617666	2
TTGCTAA	155	0.0	16.549366	10
GGGGTCC	35	0.0021689504	16.28668	6
CTATCGG	35	0.0021701597	16.285322	14
AGCGCCA	35	0.0021719749	16.283283	5
ATAAGGT	35	0.0021719749	16.283283	5
TGCTAAA	155	0.0	15.935762	11
GGTCGGT	50	8.709984E-5	15.200266	11
CCAACGA	190	0.0	15.004645	19
CTTGCTA	165	0.0	14.970584	9
AACTTGC	160	0.0	14.84463	7
GCTAAAA	175	0.0	14.658624	12
TCCAACG	260	0.0	14.619911	18
TAACTTT	300	0.0	14.56753	6
AAACTTG	170	0.0	14.5302725	6
TAGGACC	845	0.0	14.274785	4
>>END_MODULE