FastQCFastQC Report
Thu 2 Feb 2017
SRR4062684_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062684_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368591
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24510.17908929694846745No Hit
GCGCAAGACGGACCAGAGCGAAAGC22970.16783684826219084No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21440.15665746742452638No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17800.13006077052969076No Hit
GAATAACGCCGCCGCATCGCCAGTC17390.12706498873659114No Hit
GAACTACGACGGTATCTGATCGTCT17230.12589590315879617No Hit
GAATAGGACCGCGGTTCTATTTTGT17210.1257497674615718No Hit
GGGTAGGCACACGCTGAGCCAGTCA16860.12319239276014529No Hit
TATCAACGCAGAGTACTTTTTTTTT16250.11873525399480195No Hit
GATTAAGAGGGACGGCCGGGGGCAT15590.11391277598639768No Hit
GTCCTATTCCATTATTCCTAGCTGC15550.11362050459194895No Hit
GTATCTGATCGTCTTCGAACCTCCG15420.11267062255999054No Hit
TCGTAGTTCCGACCATAAACGATGC14760.10784814455158627No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC13900.10156430957093827No Hit
ATCAGATACCGTCGTAGTTCCGACC13850.10119897032787736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG250.00603748818.9968514
AAGACGG4750.014.1981685
CGTTATT1550.014.0882522
CGGACCA4950.013.8189289
CAAGACG5150.013.2793534
GCGTTAT1650.013.2321
CGACCAT2850.013.00070110
GACGGAC5250.012.6664237
CCCCCGT1352.7284841E-1112.6590112
CGAACGA1301.9645086E-1012.42738516
CGGTCCA3600.012.40344610
AGACGGA5450.012.3754566
GTATAGG701.09876266E-412.2046711
CAGAGCG5700.012.16821314
ACGGACC5550.012.1533648
GCGCAAG5550.012.1435861
GGTCCAA4000.012.11182311
GCAAGAC5500.012.0858113
ACGTAAC550.003086268412.0813911
CGCAAGA5450.012.0202532
Produced by FastQC (version 0.11.5)