##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062684_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1368591
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.233754277209187	32.0	32.0	32.0	32.0	32.0
2	30.934195826218353	32.0	32.0	32.0	32.0	32.0
3	30.905204695924496	32.0	32.0	32.0	32.0	32.0
4	30.980384936040057	32.0	32.0	32.0	32.0	32.0
5	30.7863057699488	32.0	32.0	32.0	32.0	32.0
6	34.423456679168574	36.0	36.0	36.0	32.0	36.0
7	34.32377313602092	36.0	36.0	36.0	32.0	36.0
8	34.33143722266185	36.0	36.0	36.0	32.0	36.0
9	34.492703079298344	36.0	36.0	36.0	32.0	36.0
10	34.18030953002029	36.0	36.0	36.0	32.0	36.0
11	34.44825809902301	36.0	36.0	36.0	32.0	36.0
12	34.238254526005214	36.0	36.0	36.0	32.0	36.0
13	34.36938793255253	36.0	36.0	36.0	32.0	36.0
14	34.27868004392839	36.0	36.0	36.0	32.0	36.0
15	34.20960754527832	36.0	36.0	36.0	32.0	36.0
16	34.21325290024558	36.0	36.0	36.0	32.0	36.0
17	34.134110921378266	36.0	36.0	36.0	32.0	36.0
18	34.137875376938766	36.0	36.0	36.0	32.0	36.0
19	34.13666829607969	36.0	36.0	36.0	32.0	36.0
20	34.141786698874974	36.0	36.0	36.0	32.0	36.0
21	34.128907759878594	36.0	36.0	36.0	32.0	36.0
22	34.0644063858377	36.0	36.0	36.0	32.0	36.0
23	34.04817874733941	36.0	36.0	36.0	32.0	36.0
24	34.00705177806956	36.0	36.0	36.0	32.0	36.0
25	33.5794981846293	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	59.0
5	112.0
6	223.0
7	53.0
8	199.0
9	253.0
10	154.0
11	44.0
12	109.0
13	68.0
14	170.0
15	218.0
16	327.0
17	471.0
18	603.0
19	887.0
20	1319.0
21	2028.0
22	3201.0
23	4721.0
24	6968.0
25	10057.0
26	14734.0
27	19235.0
28	26458.0
29	36420.0
30	47413.0
31	65158.0
32	95326.0
33	138549.0
34	314468.0
35	578580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.87698324544891	17.191318149399617	11.0412111681418	25.890487437009668
2	17.73864143702564	19.282100969713177	35.45813851020314	27.521119083058043
3	19.70739128560332	22.25072834316271	27.855189230385886	30.186691140848087
4	13.273769765813748	14.360441918213853	34.55273977307772	37.813048542894684
5	15.587199745053281	35.22869553967499	32.80933303803954	16.374771677232193
6	35.954169121613816	33.88675030300018	15.835030475458082	14.324050099927923
7	31.60753559270481	29.735262806939556	19.7227307822791	18.934470818076534
8	28.94693540849885	31.769280442062325	19.008358143028165	20.275426006410665
9	26.86380397531554	14.547244353627267	18.629775520025042	39.95917615103215
10	16.679358516597063	25.532271314444056	30.302431787707413	27.48593838125147
11	38.283959476788546	20.866791171154166	21.273661887654814	19.57558746440247
12	25.10869923071014	23.305568663601214	27.254224143922684	24.331507961765965
13	29.11842247718368	18.415015610780948	25.375200072567456	27.091361839467915
14	23.733463257047045	19.38562762810215	23.943345652676456	32.93756346217435
15	25.633333845369478	26.48536198394986	21.698409250006033	26.18289492067463
16	26.84364983344281	25.335857414757392	22.617398313800475	25.203094437999322
17	24.54581500182157	25.611688469399844	24.54391296268931	25.298583566089277
18	25.853581093587565	23.966188173030282	25.424275656816103	24.755955076566057
19	25.888540642412323	24.72001275928267	24.776346748138785	24.61509985016622
20	25.948213303369734	23.79772522443438	24.407384973723328	25.846676498472558
21	27.051624589938456	23.740990351665268	24.016949698142028	25.19043536025424
22	26.09540967004534	23.785024571714576	24.435172528852373	25.684393229387716
23	24.674846715542092	23.52832287048012	25.20274521854925	26.59408519542854
24	24.995939450252777	24.64270820377375	24.652292564438362	25.70905978153511
25	25.326092443522302	23.928882619889134	24.682869657399	26.062155279189565
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	94.0
1	94.0
2	271.5
3	449.0
4	449.0
5	449.0
6	1327.5
7	2206.0
8	2206.0
9	2206.0
10	2385.5
11	2565.0
12	2565.0
13	2565.0
14	2818.0
15	3071.0
16	3071.0
17	3071.0
18	4986.5
19	6902.0
20	6902.0
21	6902.0
22	11457.0
23	16012.0
24	16012.0
25	16012.0
26	24231.0
27	32450.0
28	32450.0
29	32450.0
30	39877.5
31	47305.0
32	47305.0
33	47305.0
34	61225.0
35	75145.0
36	75145.0
37	75145.0
38	87247.5
39	99350.0
40	99350.0
41	99350.0
42	115469.0
43	131588.0
44	131588.0
45	131588.0
46	153342.0
47	175096.0
48	175096.0
49	175096.0
50	180837.5
51	186579.0
52	186579.0
53	186579.0
54	175665.5
55	164752.0
56	164752.0
57	164752.0
58	155178.0
59	145604.0
60	145604.0
61	145604.0
62	130737.0
63	115870.0
64	115870.0
65	115870.0
66	95817.0
67	75764.0
68	75764.0
69	75764.0
70	57660.0
71	39556.0
72	39556.0
73	39556.0
74	31246.0
75	22936.0
76	22936.0
77	22936.0
78	18928.5
79	14921.0
80	14921.0
81	14921.0
82	10379.0
83	5837.0
84	5837.0
85	5837.0
86	4325.5
87	2814.0
88	2814.0
89	2814.0
90	1876.0
91	938.0
92	938.0
93	938.0
94	543.0
95	148.0
96	148.0
97	148.0
98	393.5
99	639.0
100	639.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0092796167737476
2	0.0030688496417118046
3	0.005407020797301751
4	0.014102094782151861
5	0.03375734605882985
6	0.04420604841037242
7	0.06539572450790632
8	0.08636619705960363
9	0.09155401431106883
10	0.1148626580183561
11	0.1148626580183561
12	0.12757646367687644
13	0.11595867574753889
14	0.11946593248092381
15	0.10960177291827872
16	0.10909029797799343
17	0.11961206817814819
18	0.12655351379630583
19	0.12735726013103987
20	0.11683548993088513
21	0.12589590315879617
22	0.14460127240351572
23	0.13524858778115595
24	0.13013383837830295
25	0.12596897100740836
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1368591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.00880371236506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63063663175339	37.74962799832149
2	11.986532403356387	11.509181626892802
3	3.6547582752929317	5.263817179640406
4	1.693970159602202	3.253019235477833
5	0.9696314770191193	2.3275423626770753
6	0.5993021567619465	1.7263067765028794
7	0.4129902637249174	1.3879017954401207
8	0.3116369127423123	1.1969052298698486
9	0.24337419206146685	1.0515693433800957
>10	1.2585342195510087	10.741523453571249
>50	0.10421239764739794	3.503106415557664
>100	0.11371736397193796	11.789889175553476
>500	0.015832123805597863	5.171403309068605
>1k	0.004871422709414728	3.3282060980465413
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2451	0.17908929694846745	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2297	0.16783684826219084	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2144	0.15665746742452638	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1780	0.13006077052969076	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1739	0.12706498873659114	No Hit
GAACTACGACGGTATCTGATCGTCT	1723	0.12589590315879617	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1721	0.1257497674615718	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1686	0.12319239276014529	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1625	0.11873525399480195	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1559	0.11391277598639768	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1555	0.11362050459194895	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1542	0.11267062255999054	No Hit
TCGTAGTTCCGACCATAAACGATGC	1476	0.10784814455158627	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1390	0.10156430957093827	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1385	0.10119897032787736	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.306784861218582E-5	0.0
5	0.0	0.0	0.0	7.306784861218582E-5	0.0
6	0.0	0.0	0.0	7.306784861218582E-5	0.0
7	0.0	0.0	0.0	7.306784861218582E-5	0.0
8	0.0	0.0	0.0	1.4613569722437164E-4	0.0
9	0.0	0.0	0.0	1.4613569722437164E-4	0.0
10	0.0	0.0	0.0	1.4613569722437164E-4	0.0
11	0.0	0.0	0.0	1.4613569722437164E-4	0.0
12	0.0	0.0	0.0	1.4613569722437164E-4	1.4613569722437164E-4
13	0.0	0.0	0.0	1.4613569722437164E-4	2.1920354583655744E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTCG	25	0.006037488	18.996851	4
AAGACGG	475	0.0	14.198168	5
CGTTATT	155	0.0	14.088252	2
CGGACCA	495	0.0	13.818928	9
CAAGACG	515	0.0	13.279353	4
GCGTTAT	165	0.0	13.232	1
CGACCAT	285	0.0	13.000701	10
GACGGAC	525	0.0	12.666423	7
CCCCCGT	135	2.7284841E-11	12.659011	2
CGAACGA	130	1.9645086E-10	12.427385	16
CGGTCCA	360	0.0	12.403446	10
AGACGGA	545	0.0	12.375456	6
GTATAGG	70	1.09876266E-4	12.204671	1
CAGAGCG	570	0.0	12.168213	14
ACGGACC	555	0.0	12.153364	8
GCGCAAG	555	0.0	12.143586	1
GGTCCAA	400	0.0	12.111823	11
GCAAGAC	550	0.0	12.085811	3
ACGTAAC	55	0.0030862684	12.081391	1
CGCAAGA	545	0.0	12.020253	2
>>END_MODULE