FastQCFastQC Report
Thu 2 Feb 2017
SRR4062684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368591
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22070.1612607418870941No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21290.1555614496953436No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18560.13561392702421687No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17250.12604203885602056No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA16450.12019661096704568No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT15520.11340130104611239No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15350.11215914761970525No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15050.10996711216133967No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC15020.1097479086155031No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG14730.10762894100574971No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG13890.1014912417223261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA2250.019.55545815
ATACCGT4300.019.4417656
AGATACG708.124344E-418.8570517
ACCGTCG4250.018.6352028
GTCCTAT4800.018.3359221
TACGCTG600.00741280318.3332425
CCTATTC4600.017.6955643
CGCAATA6150.017.17064536
CCGTAAT902.2157248E-417.11102730
TATACCA1307.318922E-716.9229935
AGCGAAA7300.016.87662717
AGAGCGA7450.016.8321315
GTATCAA25550.016.7929761
TACCGTC4850.016.783427
GCGAAAG7350.016.76182218
ATACGAA6250.016.54391740
GAGCGAA7450.016.5368316
CAATACG6600.016.33325238
CCGTCGT4850.016.3298159
CGTCGTA4850.016.32981510
Produced by FastQC (version 0.11.5)