##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062684_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1368591
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30700771815685	32.0	32.0	32.0	32.0	32.0
2	31.400042817759285	32.0	32.0	32.0	32.0	32.0
3	31.48248234863447	32.0	32.0	32.0	32.0	32.0
4	31.582240420987716	32.0	32.0	32.0	32.0	32.0
5	31.503001992560232	32.0	32.0	32.0	32.0	32.0
6	35.10659210823394	36.0	36.0	36.0	36.0	36.0
7	35.112685236129714	36.0	36.0	36.0	36.0	36.0
8	35.043435913286	36.0	36.0	36.0	36.0	36.0
9	35.19460379324429	36.0	36.0	36.0	36.0	36.0
10	35.050615560090634	36.0	36.0	36.0	36.0	36.0
11	35.185949637254666	36.0	36.0	36.0	36.0	36.0
12	35.08779905757089	36.0	36.0	36.0	36.0	36.0
13	35.14766573797431	36.0	36.0	36.0	36.0	36.0
14	35.108667235134526	36.0	36.0	36.0	36.0	36.0
15	35.06277989552759	36.0	36.0	36.0	36.0	36.0
16	35.06959858716008	36.0	36.0	36.0	36.0	36.0
17	35.044488820984505	36.0	36.0	36.0	36.0	36.0
18	35.058950409581826	36.0	36.0	36.0	36.0	36.0
19	35.04653837413807	36.0	36.0	36.0	36.0	36.0
20	35.03575794375383	36.0	36.0	36.0	36.0	36.0
21	35.036165662349084	36.0	36.0	36.0	36.0	36.0
22	35.01932717663641	36.0	36.0	36.0	36.0	36.0
23	34.98245933226216	36.0	36.0	36.0	32.0	36.0
24	34.949382247873906	36.0	36.0	36.0	32.0	36.0
25	34.92469846725574	36.0	36.0	36.0	32.0	36.0
26	34.880521645984814	36.0	36.0	36.0	32.0	36.0
27	34.866747625842926	36.0	36.0	36.0	32.0	36.0
28	34.85214574697627	36.0	36.0	36.0	32.0	36.0
29	34.82727710470111	36.0	36.0	36.0	32.0	36.0
30	34.81569585069608	36.0	36.0	36.0	32.0	36.0
31	34.81629208434076	36.0	36.0	36.0	32.0	36.0
32	34.79837657853953	36.0	36.0	36.0	32.0	36.0
33	34.777877393611384	36.0	36.0	36.0	32.0	36.0
34	34.76693475260323	36.0	36.0	36.0	32.0	36.0
35	34.73456934906046	36.0	36.0	36.0	32.0	36.0
36	34.7163871456118	36.0	36.0	36.0	32.0	36.0
37	34.705217263594456	36.0	36.0	36.0	32.0	36.0
38	34.67542311764435	36.0	36.0	36.0	32.0	36.0
39	34.68349857627297	36.0	36.0	36.0	32.0	36.0
40	34.67199623554444	36.0	36.0	36.0	32.0	36.0
41	34.64785534904146	36.0	36.0	36.0	32.0	36.0
42	34.61620820245055	36.0	36.0	36.0	32.0	36.0
43	34.61441146405318	36.0	36.0	36.0	32.0	36.0
44	34.57812962382479	36.0	36.0	36.0	32.0	36.0
45	34.541117835788775	36.0	36.0	36.0	32.0	36.0
46	34.562771492725	36.0	36.0	36.0	32.0	36.0
47	34.53446281613718	36.0	36.0	36.0	32.0	36.0
48	34.50965116678394	36.0	36.0	36.0	32.0	36.0
49	34.5103117001354	36.0	36.0	36.0	32.0	36.0
50	33.98417204263363	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	3.0
21	20.0
22	92.0
23	306.0
24	811.0
25	1974.0
26	4060.0
27	7544.0
28	12824.0
29	20231.0
30	30117.0
31	43869.0
32	65946.0
33	110972.0
34	246019.0
35	823800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.07461404951128	17.873316890472278	11.592143966383484	26.459925093632958
2	17.126063364154636	19.630202830181787	35.30361686300815	27.940116942655425
3	18.965222448896085	22.46109847842734	28.371660051556937	30.202019021119643
4	12.9364081348135	14.763328893230756	34.7625689853842	37.537693986571554
5	15.310125523257131	35.56460622640365	32.69026319769748	16.43500505264173
6	36.2421570339541	34.05361603936334	15.63324986500636	14.070977061676201
7	31.66930928912238	29.704659540110622	19.65336587290569	18.9726652978613
8	29.02927171083253	32.021765450744596	18.760827741816218	20.188135096606654
9	26.983810356783	14.255902603480514	18.57107053897037	40.189216500766115
10	16.691546269119115	25.690290232801473	30.21150950137769	27.406653996701717
11	38.69205628270243	20.663003044737252	21.174989459962838	19.46995121259748
12	25.116196146255525	23.320699902308288	27.13096900388794	24.432134947548246
13	29.433263845809304	18.235908317386276	25.24267659220322	27.0881512446012
14	23.7854844873304	19.263534540268058	23.881130301163754	33.069850671237795
15	25.837668083452254	26.549714268178004	21.36555040914342	26.24706723922633
16	27.01303749622787	25.341318187829675	22.488164835221042	25.157479480721413
17	24.796085901485544	25.67721108789989	24.249976800958066	25.276726209656502
18	26.126724492562058	24.009583579023975	25.101363372987258	24.762328555426713
19	26.039919888410783	24.691233538727055	24.498407486239497	24.770439086622666
20	26.04130817753441	23.83495142084085	24.169456031787437	25.9542843698373
21	27.308377740318328	23.826767821796285	23.724326698042	25.14052773984339
22	26.315458745527337	23.694953422899903	24.244423644463538	25.745164187109225
23	24.789071388018773	23.41145017028462	24.94017569894877	26.859302742747833
24	25.253198362403378	24.662152534979406	24.321144885506335	25.76350421711088
25	25.550730641952196	23.85234156881055	24.531141882417757	26.065785906819492
26	25.333719131574007	24.79140955917436	24.81698330618863	25.057888003063006
27	26.101589152639466	24.17559373107086	24.014332989183767	25.70848412710591
28	25.207604024869372	23.945503075791088	25.075205083184095	25.77168781615545
29	25.035967648479346	24.33035143443147	24.982701186841062	25.650979730248118
30	24.94945531572252	24.551235540786106	25.2473529345144	25.25195620897697
31	25.57425848920532	24.28322267207661	24.022881927471392	26.119636911246673
32	25.345848394443628	24.150750662542716	23.92285204272131	26.580548900292346
33	24.83218141869996	23.916202868497603	24.65499188581541	26.596623826987027
34	25.96539068282635	23.862132660524583	24.78198380670339	25.390492849945673
35	26.418484412070516	23.808427791794625	24.771681240049073	25.001406556085787
36	25.070309537327073	24.49957657181729	24.322825446024414	26.107288444831216
37	26.315458745527337	24.033769036914606	24.332908809132896	25.31786340842516
38	25.0908416027871	23.765536964659272	24.912556052173368	26.231065380380258
39	25.815017050382473	23.800755667690346	24.19093797927942	26.19328930264776
40	25.931998676010583	23.968081041012255	24.870395903524138	25.22952437945303
41	24.781837671006166	24.382594946189183	25.386547186120616	25.449020196684035
42	26.25378948129865	24.415329342367443	24.669459319840627	24.661421856493284
43	25.338833880976857	23.257423145410133	24.894727497111994	26.509015476501013
44	24.819248409495607	24.001692251373857	24.92198180464434	26.257077534486196
45	24.869957496432463	24.253337921994227	25.03801354824049	25.83869103333282
46	24.821951919894257	23.88704879690134	25.285640487187187	26.00535879601722
47	24.91562490181508	23.76546389681066	25.6083081066586	25.710603094715662
48	25.902625400868484	24.66295628131414	24.26612479550136	25.168293522316016
49	24.489566276557422	24.646077608284724	24.840365017744524	26.023991097413322
50	24.577101559194823	24.71856091410801	24.815521949216386	25.888815577480784
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	16.5
2	17.0
3	58.5
4	100.0
5	126.5
6	153.0
7	144.5
8	136.0
9	171.5
10	207.0
11	361.0
12	515.0
13	803.5
14	1092.0
15	1359.5
16	1627.0
17	1916.0
18	2205.0
19	2443.5
20	2682.0
21	3303.5
22	3925.0
23	4297.5
24	4670.0
25	5799.0
26	6928.0
27	8724.5
28	10521.0
29	12018.0
30	13515.0
31	14506.5
32	15498.0
33	17971.5
34	20445.0
35	24155.0
36	27865.0
37	35583.0
38	43301.0
39	48407.0
40	53513.0
41	59653.0
42	65793.0
43	68275.0
44	70757.0
45	78705.0
46	86653.0
47	94425.5
48	102198.0
49	105584.0
50	108970.0
51	104573.5
52	100177.0
53	99664.5
54	99152.0
55	102026.0
56	104900.0
57	102261.0
58	99622.0
59	92764.5
60	85907.0
61	75957.5
62	66008.0
63	57779.5
64	49551.0
65	42780.0
66	36009.0
67	30988.5
68	25968.0
69	24155.5
70	22343.0
71	17251.5
72	12160.0
73	10947.0
74	9734.0
75	6949.0
76	4164.0
77	3732.0
78	3300.0
79	2958.0
80	2616.0
81	2058.5
82	1501.0
83	1310.0
84	1119.0
85	879.0
86	639.0
87	467.5
88	296.0
89	187.0
90	78.0
91	51.5
92	25.0
93	18.5
94	12.0
95	7.5
96	3.0
97	3.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015782655300232137
2	0.004164867370894591
3	5.114749402853007E-4
4	2.922713944487433E-4
5	0.0
6	5.845427888974866E-4
7	7.306784861218582E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1368591.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.79091738966838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87288418569493	41.36689946535876
2	11.654367479480168	12.071807667171901
3	3.3719935326074686	5.239159154573084
4	1.5150060512864425	3.1385421298809537
5	0.8483610447413716	2.196869839240657
6	0.5597237307526733	1.7393163300269214
7	0.3755402045822181	1.3614700198411773
8	0.2826356564838813	1.1710367949065108
9	0.21875778177909863	1.0196699584021562
>10	1.0962807309535478	10.046178204080952
>50	0.09406714199610494	3.444914472767309
>100	0.09524026959033012	10.662722360128214
>500	0.011604295179877473	4.028146369844977
>1k	0.0035378948719138642	2.5132672337764577
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2207	0.1612607418870941	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2129	0.1555614496953436	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1856	0.13561392702421687	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1725	0.12604203885602056	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1645	0.12019661096704568	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1552	0.11340130104611239	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1535	0.11215914761970525	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1505	0.10996711216133967	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1502	0.1097479086155031	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1473	0.10762894100574971	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1389	0.1014912417223261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4613569722437164E-4
2	0.0	0.0	0.0	0.0	1.4613569722437164E-4
3	0.0	0.0	0.0	0.0	1.4613569722437164E-4
4	0.0	0.0	0.0	7.306784861218582E-5	1.4613569722437164E-4
5	0.0	0.0	0.0	7.306784861218582E-5	1.4613569722437164E-4
6	0.0	0.0	0.0	7.306784861218582E-5	1.4613569722437164E-4
7	0.0	0.0	0.0	7.306784861218582E-5	1.4613569722437164E-4
8	0.0	0.0	0.0	7.306784861218582E-5	1.4613569722437164E-4
9	0.0	0.0	0.0	7.306784861218582E-5	1.4613569722437164E-4
10	0.0	0.0	0.0	7.306784861218582E-5	1.4613569722437164E-4
11	0.0	0.0	0.0	7.306784861218582E-5	1.4613569722437164E-4
12	0.0	0.0	0.0	7.306784861218582E-5	3.653392430609291E-4
13	0.0	0.0	0.0	7.306784861218582E-5	4.384070916731149E-4
14	0.0	0.0	0.0	7.306784861218582E-5	4.384070916731149E-4
15	0.0	0.0	0.0	7.306784861218582E-5	5.114749402853007E-4
16	0.0	0.0	0.0	1.4613569722437164E-4	5.114749402853007E-4
17	0.0	0.0	0.0	1.4613569722437164E-4	6.576106375096723E-4
18	0.0	0.0	0.0	1.4613569722437164E-4	6.576106375096723E-4
19	0.0	0.0	0.0	2.1920354583655744E-4	6.576106375096723E-4
20	0.0	0.0	0.0	3.653392430609291E-4	7.306784861218582E-4
21	0.0	0.0	0.0	4.384070916731149E-4	8.03746334734044E-4
22	0.0	0.0	0.0	5.845427888974866E-4	8.768141833462298E-4
23	0.0	0.0	0.0	0.001607492669468088	8.768141833462298E-4
24	0.0	0.0	0.0	0.0026304425500386893	9.498820319584156E-4
25	0.0	0.0	0.0	0.003653392430609291	9.498820319584156E-4
26	0.0	0.0	0.0	0.00453020661395552	0.0011690855777949732
27	0.0	0.0	0.0	0.006429970677872352	0.001242153426407159
28	0.0	0.0	0.0	0.01395595908492749	0.001242153426407159
29	0.0	0.0	0.0	0.031199971357403343	0.001242153426407159
30	0.0	0.0	0.0	0.06422663893011134	0.001242153426407159
31	0.0	0.0	0.0	0.132910416625566	0.001242153426407159
32	0.0	0.0	0.0	0.2050283832057934	0.001242153426407159
33	0.0	0.0	0.0	0.28839879847229744	0.001242153426407159
34	0.0	0.0	0.0	0.3797336092375297	0.001242153426407159
35	0.0	0.0	0.0	0.4807864438681827	0.001242153426407159
36	0.0	0.0	0.0	0.6342289259537729	0.001242153426407159
37	0.0	0.0	0.0	0.8544554216709009	0.001242153426407159
38	0.0	0.0	0.0	1.1422696773543008	0.0013152212750193446
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	225	0.0	19.555458	15
ATACCGT	430	0.0	19.441765	6
AGATACG	70	8.124344E-4	18.85705	17
ACCGTCG	425	0.0	18.635202	8
GTCCTAT	480	0.0	18.335922	1
TACGCTG	60	0.007412803	18.333242	5
CCTATTC	460	0.0	17.695564	3
CGCAATA	615	0.0	17.170645	36
CCGTAAT	90	2.2157248E-4	17.111027	30
TATACCA	130	7.318922E-7	16.922993	5
AGCGAAA	730	0.0	16.876627	17
AGAGCGA	745	0.0	16.83213	15
GTATCAA	2555	0.0	16.792976	1
TACCGTC	485	0.0	16.78342	7
GCGAAAG	735	0.0	16.761822	18
ATACGAA	625	0.0	16.543917	40
GAGCGAA	745	0.0	16.53683	16
CAATACG	660	0.0	16.333252	38
CCGTCGT	485	0.0	16.329815	9
CGTCGTA	485	0.0	16.329815	10
>>END_MODULE