FastQCFastQC Report
Thu 2 Feb 2017
SRR4062683_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062683_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084073
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG44940.41454772879686147No Hit
TCCATGTACTCTGCGTTGATACCAC38990.35966212607453557No Hit
GTATCAACGCAGAGTACTTTTTTTT22900.21124038694811142No Hit
GAGTACATGGAAGCAGTGGTATCAA19380.17877024886700435No Hit
CATGTACTCTGCGTTGATACCACTG16580.15294172993885097No Hit
TATCAACGCAGAGTACTTTTTTTTT14240.13135646769175138No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12170.11226181262700943No Hit
GCTTCCATGTACTCTGCGTTGATAC11680.1077418218145826No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10860.10017775555705197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT508.708789E-515.2002577
CGGTTCT1250.014.4415812
AACCGGT400.00527713814.25024314
AACGGAC400.00527713814.25024315
CTCGTAG551.9590049E-413.81841710
CGACGCT500.001498843313.30022515
ACGACCG500.00150040913.2983845
CGTTATT500.001503544413.29472
AATCGTA655.44628E-513.15467613
CGTCTTA655.4486405E-513.15406915
CTAATGG957.408198E-812.996023
TCGCCAG1251.0186341E-1012.923817
CAAGACG1301.9826984E-1012.4209944
TCTATAC853.9537063E-612.2903543
CGGTCCA2350.012.12786610
TCGACGC550.00306702112.09111514
TAAGGGA1103.817513E-812.08888154
GGTTCTA1353.7107384E-1011.96371913
CGCCGGT2250.011.8224237
ATCGCCA1302.6011548E-911.69466716
Produced by FastQC (version 0.11.5)