##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062683_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1084073
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226631416887976	32.0	32.0	32.0	32.0	32.0
2	30.99813388950744	32.0	32.0	32.0	32.0	32.0
3	31.000980561272165	32.0	32.0	32.0	32.0	32.0
4	30.99559623752275	32.0	32.0	32.0	32.0	32.0
5	30.937192421543568	32.0	32.0	32.0	32.0	32.0
6	34.5070645611504	36.0	36.0	36.0	32.0	36.0
7	34.42376758760711	36.0	36.0	36.0	32.0	36.0
8	34.43326141320741	36.0	36.0	36.0	32.0	36.0
9	34.517416262557965	36.0	36.0	36.0	32.0	36.0
10	34.3212597306639	36.0	36.0	36.0	32.0	36.0
11	34.50877201074098	36.0	36.0	36.0	32.0	36.0
12	34.369068319199904	36.0	36.0	36.0	32.0	36.0
13	34.44114741350444	36.0	36.0	36.0	32.0	36.0
14	34.38372323635032	36.0	36.0	36.0	32.0	36.0
15	34.33568864827369	36.0	36.0	36.0	32.0	36.0
16	34.32381583159068	36.0	36.0	36.0	32.0	36.0
17	34.2615229786186	36.0	36.0	36.0	32.0	36.0
18	34.27183040256514	36.0	36.0	36.0	32.0	36.0
19	34.26354682756604	36.0	36.0	36.0	32.0	36.0
20	34.24543550111478	36.0	36.0	36.0	32.0	36.0
21	34.20976447158079	36.0	36.0	36.0	32.0	36.0
22	34.16267723668056	36.0	36.0	36.0	32.0	36.0
23	34.11725594125119	36.0	36.0	36.0	32.0	36.0
24	34.093535213957	36.0	36.0	36.0	32.0	36.0
25	33.57475649702557	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	9.0
4	30.0
5	120.0
6	179.0
7	39.0
8	152.0
9	217.0
10	135.0
11	32.0
12	87.0
13	80.0
14	184.0
15	344.0
16	511.0
17	735.0
18	935.0
19	1162.0
20	1601.0
21	2113.0
22	2753.0
23	3747.0
24	4909.0
25	6955.0
26	9620.0
27	12702.0
28	17397.0
29	24488.0
30	32755.0
31	47269.0
32	71439.0
33	108714.0
34	263507.0
35	469150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.72841374029604	17.292624761869618	11.014746786104718	25.96421471172962
2	16.8067622243829	20.53408510159388	36.31346965282992	26.345683021193295
3	19.432147031447037	24.034213611829376	28.254642702624416	28.278996654099164
4	12.830091198029347	15.536601454924556	36.06459975735881	35.568707589687286
5	14.438157248792082	36.54515522975332	33.80653400806877	15.210153513385821
6	33.50507424726077	35.40815921610101	17.190333413620596	13.896433123017621
7	29.59385811711449	30.58507397656824	21.181538904622474	18.639529001694797
8	28.122106083422356	32.24401273761854	20.237634695514966	19.396246483444145
9	27.392318431627466	14.364344492376787	18.583519235314974	39.65981784068077
10	15.580770555709995	26.747335785669623	32.21900723787077	25.45288642074961
11	37.27644679239707	21.5316696529176	21.824907179932392	19.36697637475294
12	23.89647097945215	24.057657757517816	29.084930190883938	22.960941072146092
13	29.530343290598648	19.733506230102325	25.327898907111397	25.408251572187634
14	23.537012476032494	19.399639425297124	25.27477196236132	31.78857613630906
15	25.349524255445687	26.564397517982986	23.018500612746795	25.067577613824525
16	25.88736153373751	25.975924532135252	23.635792749654843	24.500921184472386
17	23.90016514147859	25.599330567429075	25.78479146732083	24.715712823771508
18	24.85607115114446	24.80499292016925	26.444576424763984	23.89435950392231
19	25.151113212984654	25.864097865202577	25.419071300312574	23.565717621500195
20	25.06033220811726	24.32766415301707	26.038577517536282	24.573426121329387
21	26.081450672105213	24.750171570868698	24.394837448725017	24.773540308301072
22	25.034067151320976	25.011432705019583	25.16673272229567	24.787767421363778
23	24.21492725924051	24.58073258983708	25.315668754035634	25.888671396886775
24	23.9412993255907	25.731934079515824	25.219822700833287	25.10694389406019
25	24.765201841438273	24.664706012754102	25.198405367659554	25.371686778148067
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	103.0
1	103.0
2	341.5
3	580.0
4	580.0
5	580.0
6	1442.0
7	2304.0
8	2304.0
9	2304.0
10	2564.0
11	2824.0
12	2824.0
13	2824.0
14	3152.5
15	3481.0
16	3481.0
17	3481.0
18	5800.0
19	8119.0
20	8119.0
21	8119.0
22	12348.5
23	16578.0
24	16578.0
25	16578.0
26	24263.5
27	31949.0
28	31949.0
29	31949.0
30	39167.0
31	46385.0
32	46385.0
33	46385.0
34	57303.5
35	68222.0
36	68222.0
37	68222.0
38	77876.5
39	87531.0
40	87531.0
41	87531.0
42	100502.0
43	113473.0
44	113473.0
45	113473.0
46	133861.0
47	154249.0
48	154249.0
49	154249.0
50	149891.0
51	145533.0
52	145533.0
53	145533.0
54	133507.5
55	121482.0
56	121482.0
57	121482.0
58	110282.5
59	99083.0
60	99083.0
61	99083.0
62	87684.0
63	76285.0
64	76285.0
65	76285.0
66	63374.0
67	50463.0
68	50463.0
69	50463.0
70	38304.5
71	26146.0
72	26146.0
73	26146.0
74	20495.5
75	14845.0
76	14845.0
77	14845.0
78	11632.0
79	8419.0
80	8419.0
81	8419.0
82	5905.0
83	3391.0
84	3391.0
85	3391.0
86	2500.0
87	1609.0
88	1609.0
89	1609.0
90	1044.0
91	479.0
92	479.0
93	479.0
94	277.5
95	76.0
96	76.0
97	76.0
98	270.0
99	464.0
100	464.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009962428729430583
2	0.004704480233342219
3	0.005534682627461435
4	0.015497111356892017
5	0.03588319236804164
6	0.042801545652368427
7	0.06982924581647178
8	0.09058430566945215
9	0.09824061663744046
10	0.11954914475316698
11	0.12296219904010156
12	0.13633768205646668
13	0.1241613802760515
14	0.1251760720910861
15	0.11475241980936708
16	0.11327650444204404
17	0.12591402977474764
18	0.13098748884992062
19	0.13292462776953212
20	0.12157852838323617
21	0.13190993595449754
22	0.15238826167610484
23	0.14113440700026658
24	0.1379980868447051
25	0.13292462776953212
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1084073.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.42005618496313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16186222672064	45.79156826907816
2	11.687401776131685	13.18807729731175
3	3.085000796169783	5.221677547516654
4	1.226510617881253	2.767991918892566
5	0.6513771867925644	1.8375368738219853
6	0.4163851566426846	1.4095484359418964
7	0.28490605825418736	1.1252091069896342
8	0.20972034908787193	0.9465947102934252
9	0.16616965659923186	0.8437771225408222
>10	0.9215581684860283	9.836593308397063
>50	0.0986563875791057	3.9069387525056642
>100	0.08341831179430269	9.150291973872559
>500	0.0052340895707306675	2.010122108012873
>1k	0.0017992182899386668	1.964072574824992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4494	0.41454772879686147	No Hit
TCCATGTACTCTGCGTTGATACCAC	3899	0.35966212607453557	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2290	0.21124038694811142	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1938	0.17877024886700435	No Hit
CATGTACTCTGCGTTGATACCACTG	1658	0.15294172993885097	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1424	0.13135646769175138	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1217	0.11226181262700943	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1168	0.1077418218145826	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1086	0.10017775555705197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.224471045769058E-5
2	0.0	0.0	0.0	0.0	1.8448942091538116E-4
3	0.0	0.0	0.0	0.0	1.8448942091538116E-4
4	0.0	0.0	0.0	0.0	1.8448942091538116E-4
5	0.0	0.0	0.0	0.0	2.7673413137307176E-4
6	9.224471045769058E-5	0.0	0.0	0.0	3.6897884183076233E-4
7	9.224471045769058E-5	0.0	0.0	0.0	3.6897884183076233E-4
8	9.224471045769058E-5	0.0	0.0	0.0	3.6897884183076233E-4
9	9.224471045769058E-5	0.0	0.0	9.224471045769058E-5	3.6897884183076233E-4
10	9.224471045769058E-5	0.0	0.0	3.6897884183076233E-4	3.6897884183076233E-4
11	9.224471045769058E-5	0.0	0.0	3.6897884183076233E-4	3.6897884183076233E-4
12	9.224471045769058E-5	0.0	0.0	4.612235522884529E-4	5.534682627461435E-4
13	9.224471045769058E-5	0.0	0.0	4.612235522884529E-4	5.534682627461435E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTT	50	8.708789E-5	15.200257	7
CGGTTCT	125	0.0	14.44158	12
AACCGGT	40	0.005277138	14.250243	14
AACGGAC	40	0.005277138	14.250243	15
CTCGTAG	55	1.9590049E-4	13.818417	10
CGACGCT	50	0.0014988433	13.300225	15
ACGACCG	50	0.001500409	13.298384	5
CGTTATT	50	0.0015035444	13.2947	2
AATCGTA	65	5.44628E-5	13.154676	13
CGTCTTA	65	5.4486405E-5	13.154069	15
CTAATGG	95	7.408198E-8	12.99602	3
TCGCCAG	125	1.0186341E-10	12.9238	17
CAAGACG	130	1.9826984E-10	12.420994	4
TCTATAC	85	3.9537063E-6	12.290354	3
CGGTCCA	235	0.0	12.127866	10
TCGACGC	55	0.003067021	12.091115	14
TAAGGGA	110	3.817513E-8	12.0888815	4
GGTTCTA	135	3.7107384E-10	11.963719	13
CGCCGGT	225	0.0	11.822423	7
ATCGCCA	130	2.6011548E-9	11.694667	16
>>END_MODULE