FastQCFastQC Report
Thu 2 Feb 2017
SRR4062683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084073
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20560.18965512470101184No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18120.16714741534933533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA2500.021.99993136
GTCGCGC654.931815E-420.307638
ATATAGG1252.2873792E-819.359943
CTAGCGG2900.018.96545829
ATACGAA3100.017.7418840
GTATAGA1509.576979E-917.602381
TCGTTAG750.001290781317.59994514
CATATAG1254.8514994E-717.5999452
GTAGTAC1002.4826966E-517.5999453
TCTAGCG3150.017.46026228
TACCGTC2300.017.2173377
TATTAGG902.2151586E-417.1110572
CGGTCCA3550.016.73234210
GATAGTA800.001988213716.4999492
GTACGAA800.001988213716.49994925
ACTACCC953.3459958E-416.2104743
TAGCGGC3400.016.1764230
ATGGGTG4550.015.9559935
ATACCGT2353.6379788E-1215.9148436
GTATTAC1259.426289E-615.8421421
Produced by FastQC (version 0.11.5)