##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062683_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1084073
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249405713452877	32.0	32.0	32.0	32.0	32.0
2	31.331281196008018	32.0	32.0	32.0	32.0	32.0
3	31.443094699342204	32.0	32.0	32.0	32.0	32.0
4	31.57205557190337	32.0	32.0	32.0	32.0	32.0
5	31.482179705610232	32.0	32.0	32.0	32.0	32.0
6	35.08363366673647	36.0	36.0	36.0	36.0	36.0
7	35.10366829540077	36.0	36.0	36.0	36.0	36.0
8	35.036450497337356	36.0	36.0	36.0	36.0	36.0
9	35.19217340529651	36.0	36.0	36.0	36.0	36.0
10	35.045888053664285	36.0	36.0	36.0	36.0	36.0
11	35.19643880070807	36.0	36.0	36.0	36.0	36.0
12	35.09373722987289	36.0	36.0	36.0	36.0	36.0
13	35.14221735990104	36.0	36.0	36.0	36.0	36.0
14	35.10404096403102	36.0	36.0	36.0	36.0	36.0
15	35.05906428810606	36.0	36.0	36.0	36.0	36.0
16	35.06443662004312	36.0	36.0	36.0	36.0	36.0
17	35.04497298613654	36.0	36.0	36.0	36.0	36.0
18	35.05653586059241	36.0	36.0	36.0	36.0	36.0
19	35.04369170710829	36.0	36.0	36.0	36.0	36.0
20	35.02980611084309	36.0	36.0	36.0	36.0	36.0
21	35.01964443353907	36.0	36.0	36.0	36.0	36.0
22	34.99548093163468	36.0	36.0	36.0	36.0	36.0
23	34.95019062369416	36.0	36.0	36.0	32.0	36.0
24	34.92985435482666	36.0	36.0	36.0	32.0	36.0
25	34.906834687331944	36.0	36.0	36.0	32.0	36.0
26	34.85723378407174	36.0	36.0	36.0	32.0	36.0
27	34.83094680893261	36.0	36.0	36.0	32.0	36.0
28	34.815175730785654	36.0	36.0	36.0	32.0	36.0
29	34.79141533826596	36.0	36.0	36.0	32.0	36.0
30	34.77414620602118	36.0	36.0	36.0	32.0	36.0
31	34.77046748696813	36.0	36.0	36.0	32.0	36.0
32	34.73059102108437	36.0	36.0	36.0	32.0	36.0
33	34.70559731678586	36.0	36.0	36.0	32.0	36.0
34	34.69132706007806	36.0	36.0	36.0	32.0	36.0
35	34.65460351839775	36.0	36.0	36.0	32.0	36.0
36	34.61414222104969	36.0	36.0	36.0	32.0	36.0
37	34.608842762433895	36.0	36.0	36.0	32.0	36.0
38	34.56375354796218	36.0	36.0	36.0	32.0	36.0
39	34.52857510518203	36.0	36.0	36.0	32.0	36.0
40	34.49645457455356	36.0	36.0	36.0	32.0	36.0
41	34.47476784312496	36.0	36.0	36.0	32.0	36.0
42	34.415559653270584	36.0	36.0	36.0	32.0	36.0
43	34.417322449687425	36.0	36.0	36.0	32.0	36.0
44	34.40255776133157	36.0	36.0	36.0	32.0	36.0
45	34.31663919311707	36.0	36.0	36.0	32.0	36.0
46	34.36361112212923	36.0	36.0	36.0	32.0	36.0
47	34.31141814250516	36.0	36.0	36.0	32.0	36.0
48	34.285439264698965	36.0	36.0	36.0	32.0	36.0
49	34.293387068952	36.0	36.0	36.0	32.0	36.0
50	33.72319207285856	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	4.0
21	21.0
22	80.0
23	262.0
24	616.0
25	1567.0
26	3468.0
27	6490.0
28	11302.0
29	17593.0
30	26106.0
31	37837.0
32	56762.0
33	91892.0
34	202013.0
35	628057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0189277532934	17.991421804926787	11.490150021542359	26.499500420237453
2	16.22389272690877	21.018839762849424	35.97939922714815	26.77786828309366
3	18.802712378478454	24.403288726619294	28.650350946943316	28.14364794795894
4	12.58044906652793	16.042768416459808	36.25721931497107	35.11956320204119
5	14.323574150449279	36.688119711495446	33.73259918843104	15.25570694962424
6	33.87650394302197	35.701667885841005	16.859197817109088	13.562630354027933
7	29.733145277116947	30.608455334649975	21.175234509115164	18.48316487911792
8	28.264978465472346	32.36802318663042	20.083702850269308	19.28329549762793
9	27.522408546287934	14.094899513224663	18.42163765724264	39.96105428324476
10	15.443978403668387	26.76840028300677	32.309909019042074	25.477712294282767
11	37.52044373395518	21.36304473960702	21.86909922117791	19.247412305259886
12	23.894331839276507	23.949863154972036	29.13669097929752	23.019114026453938
13	29.66036420056583	19.65863922448027	25.32338689368705	25.35760968126685
14	23.594721019709926	19.33273866243325	25.157254170152747	31.915286147704077
15	25.45585029790429	26.748844404389743	22.819957696575784	24.97534760113018
16	26.069831090710682	25.908587336830635	23.60864997098904	24.41293160146964
17	24.090536338420016	25.665245790643247	25.552614999174413	24.691602871762324
18	25.043055218606124	24.904226929367304	26.327747301150385	23.724970550876186
19	25.319604860558282	25.93460034517971	25.211217325770498	23.534577468491513
20	25.19479776730903	24.315705676647237	25.872704144462595	24.61679241158114
21	26.296199610173854	24.761432117578796	24.258329466742552	24.684038805504795
22	25.17431944158742	24.99370429851126	24.964185991164804	24.867790268736513
23	24.339043588393032	24.53847665240256	25.119433838865096	26.003045920339314
24	24.17779983451299	25.73276891869828	25.047206230576723	25.042225016212004
25	25.084842072443458	24.579525548556234	25.06381027845911	25.271822100541204
26	24.292828988453728	25.075156377845403	25.586653297333296	25.045361336367574
27	25.518945679857353	25.26305885304772	24.862716809661343	24.355278657433583
28	24.572238216430076	24.420218933595798	25.69753143930344	25.310011410670686
29	24.26755393778832	25.409635697964987	25.718839967419164	24.60397039682752
30	25.002283056583828	24.91787914651504	25.655560096045193	24.42427770085594
31	24.382490847018605	25.112515485580765	24.970735365607297	25.53425830179333
32	24.426860552748757	25.371354143125046	25.047851943549926	25.153933360576268
33	24.331295032714586	24.846112761778958	25.237414823540483	25.585177381965973
34	24.409703036603624	25.124230563808897	25.975095773070635	24.49097062651685
35	25.794941853546767	24.668357204726988	25.169891695485454	24.3668092462408
36	24.64907806024133	25.308258761171988	25.072758015373502	24.96990516321318
37	25.543667262260012	25.076540048502267	25.039734409029652	24.34005828020807
38	24.64234419637792	24.80681651512398	25.18160677371358	25.369232514784525
39	24.64511153769165	25.00126836476879	25.237414823540483	25.116205273999075
40	25.25900008578758	24.998501023455063	25.186587988078298	24.555910902679063
41	24.08712328413308	25.29386858634059	25.70352734548319	24.91548078404314
42	25.488135946564483	25.19489001201949	25.373291282044658	23.94368275937137
43	24.806355291571695	24.172818620148274	25.366649662891703	25.654176425388325
44	24.313030580043964	24.765214150707564	25.890691863001848	25.031063406246627
45	24.708022430223796	24.97710025062888	25.47918820965009	24.83568910949724
46	24.293150540877857	24.849756150658028	25.852458003211964	25.004635305252148
47	24.541243993716293	24.595945107017698	26.203124697322046	24.659686201943963
48	24.722966073317938	25.93312442981238	24.884117582487526	24.45979191438215
49	23.76315986100567	25.049235614206793	26.21825282983711	24.969351694950433
50	23.90586242808372	25.848443785612226	24.988538594725632	25.257155191578427
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	13.0
2	18.0
3	52.5
4	87.0
5	103.5
6	120.0
7	127.0
8	134.0
9	190.0
10	246.0
11	349.0
12	452.0
13	774.0
14	1096.0
15	1504.5
16	1913.0
17	2330.5
18	2748.0
19	3131.5
20	3515.0
21	4287.0
22	5059.0
23	5528.5
24	5998.0
25	7001.5
26	8005.0
27	10074.5
28	12144.0
29	13661.5
30	15179.0
31	16445.5
32	17712.0
33	20127.5
34	22543.0
35	26194.5
36	29846.0
37	35149.0
38	40452.0
39	44248.5
40	48045.0
41	53099.0
42	58153.0
43	59641.0
44	61129.0
45	68062.0
46	74995.0
47	80209.5
48	85424.0
49	85664.5
50	85905.0
51	82030.5
52	78156.0
53	75336.5
54	72517.0
55	72559.5
56	72602.0
57	70390.5
58	68179.0
59	62322.0
60	56465.0
61	49756.0
62	43047.0
63	37860.5
64	32674.0
65	28075.0
66	23476.0
67	20852.5
68	18229.0
69	16090.0
70	13951.0
71	11247.5
72	8544.0
73	7412.0
74	6280.0
75	4700.5
76	3121.0
77	2681.0
78	2241.0
79	1945.0
80	1649.0
81	1261.5
82	874.0
83	722.0
84	570.0
85	460.5
86	351.0
87	250.0
88	149.0
89	96.5
90	44.0
91	31.0
92	18.0
93	10.0
94	2.0
95	2.5
96	3.0
97	3.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014943643094145873
2	0.003320809576476861
3	5.534682627461435E-4
4	1.8448942091538116E-4
5	0.0
6	5.534682627461435E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.8448942091538116E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1084073.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.263247371051555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.87601326264156	49.34114441002067
2	11.443757031272677	13.792759216595918
3	2.975391494569966	5.37920260988978
4	1.1459005950035648	2.7622276407734
5	0.5902608493830966	1.7785517789910281
6	0.3959611474113031	1.431714274546366
7	0.2554561362089765	1.0776231430170213
8	0.19150620698234402	0.9232628739575038
9	0.13878923561752313	0.752750103461216
>10	0.8344937437075987	9.48568409983251
>50	0.07886380369586495	3.329426412253743
>100	0.06930381865466982	7.987804877580162
>500	0.003995340932997575	1.5996535810028185
>1k	3.0733391792289033E-4	0.35819497807797285
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2056	0.18965512470101184	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1812	0.16714741534933533	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	9.224471045769058E-5	0.0	0.0	0.0	0.0
7	9.224471045769058E-5	0.0	0.0	0.0	0.0
8	9.224471045769058E-5	0.0	0.0	0.0	0.0
9	9.224471045769058E-5	0.0	0.0	0.0	0.0
10	9.224471045769058E-5	0.0	0.0	9.224471045769058E-5	0.0
11	9.224471045769058E-5	0.0	0.0	9.224471045769058E-5	0.0
12	9.224471045769058E-5	0.0	0.0	1.8448942091538116E-4	9.224471045769058E-5
13	9.224471045769058E-5	0.0	0.0	1.8448942091538116E-4	9.224471045769058E-5
14	9.224471045769058E-5	0.0	0.0	1.8448942091538116E-4	1.8448942091538116E-4
15	9.224471045769058E-5	0.0	0.0	1.8448942091538116E-4	2.7673413137307176E-4
16	1.8448942091538116E-4	0.0	0.0	2.7673413137307176E-4	4.612235522884529E-4
17	1.8448942091538116E-4	0.0	0.0	2.7673413137307176E-4	4.612235522884529E-4
18	1.8448942091538116E-4	0.0	0.0	3.6897884183076233E-4	4.612235522884529E-4
19	1.8448942091538116E-4	0.0	0.0	4.612235522884529E-4	4.612235522884529E-4
20	1.8448942091538116E-4	0.0	0.0	7.379576836615247E-4	5.534682627461435E-4
21	1.8448942091538116E-4	0.0	0.0	9.224471045769058E-4	6.45712973203834E-4
22	1.8448942091538116E-4	0.0	0.0	0.0014759153673230493	6.45712973203834E-4
23	1.8448942091538116E-4	0.0	0.0	0.002213873050984574	6.45712973203834E-4
24	1.8448942091538116E-4	0.0	0.0	0.0033208095764768607	6.45712973203834E-4
25	1.8448942091538116E-4	0.0	0.0	0.003874277839223004	6.45712973203834E-4
26	1.8448942091538116E-4	0.0	0.0	0.0048889696542576	6.45712973203834E-4
27	1.8448942091538116E-4	0.0	0.0	0.006826108573869103	6.45712973203834E-4
28	1.8448942091538116E-4	0.0	0.0	0.01282201475361899	6.45712973203834E-4
29	1.8448942091538116E-4	0.0	0.0	0.024352603560830313	6.45712973203834E-4
30	1.8448942091538116E-4	0.0	0.0	0.04455419515106455	6.45712973203834E-4
31	1.8448942091538116E-4	0.0	0.0	0.08947736914395986	6.45712973203834E-4
32	1.8448942091538116E-4	0.0	0.0	0.14528541897086267	6.45712973203834E-4
33	1.8448942091538116E-4	0.0	0.0	0.20607468316248076	6.45712973203834E-4
34	1.8448942091538116E-4	0.0	0.0	0.27645739724169865	7.379576836615247E-4
35	1.8448942091538116E-4	0.0	0.0	0.35006867618693577	7.379576836615247E-4
36	1.8448942091538116E-4	0.0	0.0	0.46177702055119907	7.379576836615247E-4
37	1.8448942091538116E-4	0.0	0.0	0.6376876833940149	7.379576836615247E-4
38	1.8448942091538116E-4	0.0	0.0	0.8555696894950802	7.379576836615247E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	250	0.0	21.999931	36
GTCGCGC	65	4.931815E-4	20.30763	8
ATATAGG	125	2.2873792E-8	19.35994	3
CTAGCGG	290	0.0	18.965458	29
ATACGAA	310	0.0	17.74188	40
GTATAGA	150	9.576979E-9	17.60238	1
TCGTTAG	75	0.0012907813	17.599945	14
CATATAG	125	4.8514994E-7	17.599945	2
GTAGTAC	100	2.4826966E-5	17.599945	3
TCTAGCG	315	0.0	17.460262	28
TACCGTC	230	0.0	17.217337	7
TATTAGG	90	2.2151586E-4	17.111057	2
CGGTCCA	355	0.0	16.732342	10
GATAGTA	80	0.0019882137	16.499949	2
GTACGAA	80	0.0019882137	16.499949	25
ACTACCC	95	3.3459958E-4	16.210474	3
TAGCGGC	340	0.0	16.17642	30
ATGGGTG	455	0.0	15.955993	5
ATACCGT	235	3.6379788E-12	15.914843	6
GTATTAC	125	9.426289E-6	15.842142	1
>>END_MODULE