FastQCFastQC Report
Thu 2 Feb 2017
SRR4062682_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062682_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704835
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45870.6507906105684309No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31030.4402448800073776No Hit
TATCAACGCAGAGTACTTTTTTTTT27150.38519653535933945No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24440.34674782041186947No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19970.28332872232508316No Hit
GGTATCAACGCAGAGTACTTTTTTT16070.22799662332318912No Hit
GTACATGGAAGCAGTGGTATCAACG14530.20614753807628736No Hit
TCCATGTACTCTGCGTTGATACCAC12090.1715295069058716No Hit
GTACATGGGGTGGTATCAACGCAAA10690.1516667021359609No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7850.11137358388842779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG359.944351E-518.9963785
TAACTTT453.5402365E-516.885676
GTATCTA350.002168289216.2860811
GCGTTAT508.743759E-515.1917091
CCCTTAC456.783841E-414.7697171
TAGGACG400.005284473314.2462734
CGGTCCA707.247376E-613.571732510
TGTGCGG500.001497873813.30029810
GTTATAC500.00150108413.2965213
CGACCAT655.4430828E-513.15414110
CAGTACT802.0008447E-613.0590844
GCCGGTC751.4776811E-512.6651538
TAAACTG604.093666E-412.6642535
GCCCGTG604.093666E-412.6642537
GTATTAG1001.4483157E-712.3432641
GCAATAC701.09193075E-412.211093
TATTAGC701.09265486E-412.2102232
TATACAC951.0403637E-611.99686053
CCGGTCC1001.9239396E-611.4002559
TAAGGTG752.0744145E-411.3978275