##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062682_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	704835
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.948789432987862	32.0	32.0	32.0	32.0	32.0
2	30.76891612930686	32.0	32.0	32.0	32.0	32.0
3	30.781510566302753	32.0	32.0	32.0	32.0	32.0
4	30.77072506331269	32.0	32.0	32.0	32.0	32.0
5	30.70471245043166	32.0	32.0	32.0	32.0	32.0
6	34.2734540708109	36.0	36.0	36.0	32.0	36.0
7	34.09612888122752	36.0	36.0	36.0	32.0	36.0
8	34.087946824434084	36.0	36.0	36.0	32.0	36.0
9	34.165630253889205	36.0	36.0	36.0	32.0	36.0
10	33.97056899841807	36.0	36.0	36.0	32.0	36.0
11	34.2667390240269	36.0	36.0	36.0	32.0	36.0
12	34.08243347733867	36.0	36.0	36.0	32.0	36.0
13	34.16851036058085	36.0	36.0	36.0	32.0	36.0
14	34.09817191257528	36.0	36.0	36.0	32.0	36.0
15	34.080372001957905	36.0	36.0	36.0	32.0	36.0
16	34.079150439464556	36.0	36.0	36.0	32.0	36.0
17	34.01722388927905	36.0	36.0	36.0	32.0	36.0
18	34.040998247816866	36.0	36.0	36.0	32.0	36.0
19	34.020579284513396	36.0	36.0	36.0	32.0	36.0
20	33.95917058602368	36.0	36.0	36.0	32.0	36.0
21	33.917739612817186	36.0	36.0	36.0	32.0	36.0
22	33.906027651861784	36.0	36.0	36.0	32.0	36.0
23	33.88774819638639	36.0	36.0	36.0	32.0	36.0
24	33.889525917413295	36.0	36.0	36.0	32.0	36.0
25	33.4455042669561	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	41.0
5	54.0
6	118.0
7	38.0
8	113.0
9	139.0
10	77.0
11	22.0
12	51.0
13	30.0
14	282.0
15	308.0
16	430.0
17	513.0
18	767.0
19	998.0
20	1554.0
21	2052.0
22	3009.0
23	4072.0
24	5170.0
25	6592.0
26	8809.0
27	10958.0
28	14437.0
29	18826.0
30	24014.0
31	32260.0
32	45980.0
33	66298.0
34	150762.0
35	306058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.22233510316782	17.637710812260384	11.209976531483852	24.92997755308794
2	16.458029173778502	20.933271472777808	37.27297105181389	25.3357283016298
3	19.61021621705985	25.192217377671145	28.206969059351504	26.9905973459175
4	12.697984748682467	15.827002267547954	35.70110936732858	35.773903616441
5	14.012827445453551	37.26289166783757	33.33957816183473	15.384702724874144
6	32.75548079857489	35.41238174320977	17.468577227982767	14.363560230232576
7	28.95101122319318	31.44368171847603	21.19955419574214	18.405752862588646
8	28.03577336906126	32.264477550052476	20.279149289062513	19.420599791823754
9	27.575115067322013	14.635881690484881	19.343381338998768	38.44562190319433
10	15.82307879152894	27.5799470736381	32.129114186566134	24.467859948266828
11	35.68513533669041	22.491104213868194	22.326185927256972	19.497574522184422
12	24.23559228368359	24.93639110626201	29.146440388060423	21.68157622199397
13	29.217508402203467	20.686892562593933	25.89483822029541	24.2007608149072
14	23.254991370309604	20.182253379074243	26.597060933427088	29.96569431718906
15	24.538263447282564	27.129925414792332	24.497640005283888	23.83417113264122
16	24.081562504882474	26.920711484569964	25.216568733142154	23.781157277405406
17	22.6929349061321	26.39708054911587	26.504471794172463	24.40551275057957
18	23.020327680678264	25.864270929669264	28.05146061254381	23.063940777108666
19	23.90304955491216	26.086011484487987	27.550695689481262	22.46024327111859
20	24.477043316694555	24.916971837188093	27.614215216952804	22.991769629164548
21	25.349513501073297	24.900874689412095	26.414142777383944	23.335469032130664
22	24.242273541702485	25.401863926806982	26.81991466119528	23.53594787029525
23	23.506863639334295	25.359236432555615	27.094965717322054	24.03893421078803
24	23.177583901961537	26.100496244425596	27.102787805428996	23.61913204818387
25	23.573067388354964	25.757168936149057	27.18264538538581	23.48711829011017
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	461.0
1	461.0
2	792.5
3	1124.0
4	1124.0
5	1124.0
6	2761.0
7	4398.0
8	4398.0
9	4398.0
10	4417.5
11	4437.0
12	4437.0
13	4437.0
14	3882.0
15	3327.0
16	3327.0
17	3327.0
18	5183.0
19	7039.0
20	7039.0
21	7039.0
22	10476.0
23	13913.0
24	13913.0
25	13913.0
26	19409.0
27	24905.0
28	24905.0
29	24905.0
30	30418.5
31	35932.0
32	35932.0
33	35932.0
34	41300.5
35	46669.0
36	46669.0
37	46669.0
38	52344.0
39	58019.0
40	58019.0
41	58019.0
42	66160.0
43	74301.0
44	74301.0
45	74301.0
46	85536.5
47	96772.0
48	96772.0
49	96772.0
50	95718.0
51	94664.0
52	94664.0
53	94664.0
54	85592.5
55	76521.0
56	76521.0
57	76521.0
58	68389.0
59	60257.0
60	60257.0
61	60257.0
62	52065.5
63	43874.0
64	43874.0
65	43874.0
66	35966.5
67	28059.0
68	28059.0
69	28059.0
70	21416.0
71	14773.0
72	14773.0
73	14773.0
74	11308.5
75	7844.0
76	7844.0
77	7844.0
78	6159.0
79	4474.0
80	4474.0
81	4474.0
82	3082.0
83	1690.0
84	1690.0
85	1690.0
86	1242.5
87	795.0
88	795.0
89	795.0
90	517.5
91	240.0
92	240.0
93	240.0
94	147.0
95	54.0
96	54.0
97	54.0
98	173.5
99	293.0
100	293.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008654507792603943
2	0.0034050522462704034
3	0.004823824015549739
4	0.01546461228514475
5	0.035753048585839234
6	0.04681946838621805
7	0.06951981669468742
8	0.0885313584030305
9	0.09676023466485063
10	0.11889307426560826
11	0.12059560038874347
12	0.13109451148141055
13	0.12102123191952727
14	0.12343314392730213
15	0.11463675895777026
16	0.11151546106535572
17	0.12300751239651833
18	0.13024324841984294
19	0.13180389736605022
20	0.121588740627239
21	0.1316620201891223
22	0.14925479012818602
23	0.13918151056630276
24	0.13449956372768096
25	0.13194577454297815
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	704835.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.83431383193674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.56097604850106	53.52784231399531
2	11.680972341385177	15.14655653287079
3	2.793126867421819	5.432713916845218
4	1.0271507660459276	2.663784604741436
5	0.5046333667844878	1.635877903608616
6	0.2831260321409528	1.1013769213090525
7	0.1996926351306552	0.9062854483191135
8	0.1444134865564664	0.7490359447172897
9	0.10628436722371383	0.6201786618009967
>10	0.5894791562711813	7.251192015783296
>50	0.0696926976798322	3.206831062546408
>100	0.036934649219378404	4.2384997703422895
>500	0.0015389437172356633	0.6428448332822555
>1k	0.0019786419221601383	2.8769800698379253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4587	0.6507906105684309	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3103	0.4402448800073776	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2715	0.38519653535933945	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2444	0.34674782041186947	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1997	0.28332872232508316	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1607	0.22799662332318912	No Hit
GTACATGGAAGCAGTGGTATCAACG	1453	0.20614753807628736	No Hit
TCCATGTACTCTGCGTTGATACCAC	1209	0.1715295069058716	No Hit
GTACATGGGGTGGTATCAACGCAAA	1069	0.1516667021359609	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	785	0.11137358388842779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4187717692793348E-4
2	0.0	0.0	0.0	0.0	1.4187717692793348E-4
3	0.0	0.0	0.0	0.0	1.4187717692793348E-4
4	0.0	0.0	0.0	0.0	1.4187717692793348E-4
5	0.0	0.0	0.0	1.4187717692793348E-4	1.4187717692793348E-4
6	0.0	0.0	0.0	1.4187717692793348E-4	2.8375435385586697E-4
7	0.0	0.0	0.0	1.4187717692793348E-4	2.8375435385586697E-4
8	0.0	0.0	0.0	1.4187717692793348E-4	2.8375435385586697E-4
9	0.0	0.0	0.0	4.256315307838005E-4	2.8375435385586697E-4
10	0.0	0.0	0.0	4.256315307838005E-4	2.8375435385586697E-4
11	0.0	0.0	0.0	4.256315307838005E-4	2.8375435385586697E-4
12	0.0	0.0	0.0	4.256315307838005E-4	4.256315307838005E-4
13	0.0	0.0	0.0	5.675087077117339E-4	5.675087077117339E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTACCG	35	9.944351E-5	18.996378	5
TAACTTT	45	3.5402365E-5	16.88567	6
GTATCTA	35	0.0021682892	16.28608	11
GCGTTAT	50	8.743759E-5	15.191709	1
CCCTTAC	45	6.783841E-4	14.769717	1
TAGGACG	40	0.0052844733	14.246273	4
CGGTCCA	70	7.247376E-6	13.5717325	10
TGTGCGG	50	0.0014978738	13.300298	10
GTTATAC	50	0.001501084	13.296521	3
CGACCAT	65	5.4430828E-5	13.154141	10
CAGTACT	80	2.0008447E-6	13.059084	4
GCCGGTC	75	1.4776811E-5	12.665153	8
TAAACTG	60	4.093666E-4	12.664253	5
GCCCGTG	60	4.093666E-4	12.664253	7
GTATTAG	100	1.4483157E-7	12.343264	1
GCAATAC	70	1.09193075E-4	12.21109	3
TATTAGC	70	1.09265486E-4	12.210223	2
TATACAC	95	1.0403637E-6	11.9968605	3
CCGGTCC	100	1.9239396E-6	11.400255	9
TAAGGTG	75	2.0744145E-4	11.397827	5
>>END_MODULE