FastQCFastQC Report
Thu 2 Feb 2017
SRR4062682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704835
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11100.15748366639000616No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8970.12726382770435635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA353.216634E-431.428375
ATAGTAC508.336951E-526.399833
CGTCGTA1004.6175046E-821.99985910
ACCGTCG1158.756615E-921.0433438
ATACCGT1101.263852E-719.9998726
TACCGTC1152.0185507E-719.1303127
CGAACGA600.007409389618.33321416
CCGATAA600.007409389618.3332149
CGAGACT600.007409389618.33321420
CGATAAC600.007409389618.33321410
CGGGTTA851.4298318E-418.1175343
TATAGTA750.001289588517.6011352
TATGGCG750.00129020217.59988616
ATAACGC1509.576979E-917.5998863
AAGACGG1651.6552804E-917.3332215
GAGCGAA1651.6552804E-917.33322116
GACGGAC1651.6552804E-917.3332217
CGACCAT1307.306644E-716.92296821
CGTGGAC800.00198732816.4998933
TAATACT1206.3993157E-616.4998934
Produced by FastQC (version 0.11.5)