FastQCFastQC Report
Thu 2 Feb 2017
SRR4062681_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062681_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397852
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT164881.179524012556408No Hit
GTACATGGAAGCAGTGGTATCAACG145501.0408827257821285No Hit
TCCATGTACTCTGCGTTGATACCAC126220.9029568223245379No Hit
TATCAACGCAGAGTACTTTTTTTTT105580.7553017057599803No Hit
GTACTTTTTTTTTTTTTTTTTTTTT98650.7057256419134501No Hit
GAGTACTTTTTTTTTTTTTTTTTTT67800.48502988871497127No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63230.4523368711422954No Hit
GAGTACATGGAAGCAGTGGTATCAA62250.4453261146387458No Hit
GGTATCAACGCAGAGTACTTTTTTT61070.4368845915018185No Hit
CCATAGGGTCTTCTCGTCTTATTAT57640.4123469437393944No Hit
GTACATGGGGTGGTATCAACGCAAA56300.40276080729576524No Hit
CATGTACTCTGCGTTGATACCACTG51610.3692093297430629No Hit
GTACATGGGAAGCAGTGGTATCAAC41940.300031763019261No Hit
GTATTAGAGGCACTGCCTGCCCAGT41880.2996025330292477No Hit
CCCATGTACTCTGCGTTGATACCAC41810.2991017647075656No Hit
GCTTCCATGTACTCTGCGTTGATAC38320.2741348869551283No Hit
GCGTTGATACCACTGCTTCCATGTA36930.2641910588531547No Hit
CATGGAAGCAGTGGTATCAACGCAG35650.25503415239953875No Hit
GTCAGGATACCGCGGCCGTTAAACT33970.24301571267916774No Hit
GTACATGGGTGGTATCAACGCAAAA33910.2425864826891545No Hit
CCATTGGGATGTCCTGATCCAACAT33810.24187109937246573No Hit
GTATCAACGCAGAGTACATGGGAAG33660.24079802439743264No Hit
CTCTAATACTTGTAATGCTAGAGGT33190.2374357228089955No Hit
GTATCAACGCAGAGTACATGGAAGC32690.2338588062255518No Hit
GTATCAACGCAGAGTACATGGGGTG32160.23006727464710142No Hit
CTGTTAGTATGAGTAACAAGAATTC30130.2155449933183198No Hit
ATCTATAACTTTATAGATGCAACAC28570.20438501357797537No Hit
GTATCCTGACCGTGCAAAGGTAGCA27220.19472733880267723No Hit
ATCCTGACCGTGCAAAGGTAGCATA27210.1946558004710084No Hit
CTTCTACACCATTGGGATGTCCTGA27090.19379734049098188No Hit
GTATCAACGCAGAGTACATGGGAGA26980.19301041884262424No Hit
GCAGAGTACTTTTTTTTTTTTTTTT26540.18986273224919376No Hit
GATATATTTTGATCAACGGACCAAG26450.1892188872641739No Hit
ACTCTGCGTTGATACCACTGCTTCC26050.1863573539974189No Hit
GTACATGGGAGTGGTATCAACGCAA25100.17956121248887577No Hit
ACGCAGAGTACATGGAAGCAGTGGT24950.17848813751384268No Hit
GTTATATAATTTAAGCTCCATAGGG24880.17798736919216054No Hit
CTATAGAACTAGTACCGCAAGGGAA24600.17598429590543205No Hit
GTTATAGATTAACCCAATTTTAAGT24290.17376660762369694No Hit
ATAAATAATCCACCTATAACTTCTC24050.17204968766364395No Hit
CCTCTAGCATTACAAGTATTAGAGG24030.1719066110003062No Hit
GCCTAAAGGAAAGATCCAAAAAGAT23800.17026122937192206No Hit
ACCTATAACTTCTCTGTTAACCCAA23780.1701181527085843No Hit
GTTCATGCTAGTCCCTAATTAAGGA23640.16911661606522008No Hit
CAGTGGTATCAACGCAGAGTACATG23190.1658973911401207No Hit
TGCTAGAGGTGATGTTTTTGGTAAA23130.16546816115010746No Hit
ATCGTAAATAGATAGAAACCGACCT22860.16353662619504783No Hit
CCTATAACTTCTCTGTTAACCCAAC22490.16088970792329946No Hit
CACTATAAATAATCCACCTATAACT22300.15953047962159084No Hit
GGTCAGGATACCGCGGCCGTTAAAC22070.1578850979932067No Hit
GATTAAAGATAAGAGACAGTTGGAC22050.15774202132986898No Hit
GATTAAACCTTGTACCTTTTGCATA22000.1573843296715246No Hit
GTACATGGGAGAAATCGTAAATAGA21640.1548089497314451No Hit
CTGTTAACCCAACACCGGAATGCCT21390.15302049143972324No Hit
GACTATAGGCAATAATCACACTATA20610.147440501569551No Hit
TATCAACGCAGAGTACATGGGGTGG20450.146295888262849No Hit
ATCAACGCAGAGTACTTTTTTTTTT20010.1431482016694185No Hit
TATCAACGCAGAGTACATGGAAGCA19620.14035820673433239No Hit
GTGTAGAAGCTATTAATGGTTCGTT19600.14021513007099465No Hit
TCCCAATGGTGTAGAAGCTATTAAT19590.14014359173932575No Hit
GATAGAAACCGACCTGGATTGCTCC19410.138855901769286No Hit
GTTAGTATGAGTAACAAGAATTCCA19400.13878436343761716No Hit
GTCCTGATCCAACATCGAGGTCGTA19020.13606590683419992No Hit
AGCATGAACGGCTAAACGAGGGTCC18870.13499283185916677No Hit
GACCTGGATTGCTCCGGTCTGAACT18710.13384821855246476No Hit
TATCAACGCAGAGTACATGGGAAGC18500.1323459135874184No Hit
GGTATCAACGCAGAGTACATGGGAA18300.1309151469540409No Hit
TACTAACAGTGTTGCATCTATAAAG18290.13084360862237204No Hit
GCTATCACCAAGCTCGTTAGGCTTT18010.12884053533564355No Hit
CTTTATTGGTGGCTGCTTTTAGGCC17900.12805361368728593No Hit
CCTATAGTCTGATTAACTAACAATG17770.12712361537559053No Hit
GTATCAACGCAGAGTACATGGGAGT17090.12225900882210707No Hit
GGTATCAACGCAGAGTACATGGGGT17040.12190131716376268No Hit
GGTATCAACGCAGAGTACATGGGAG16810.12025593553537857No Hit
GATATACACTGTTCTACAAATCCCG16640.1190397838970077No Hit
CTATTAAAGGTTTTTTCCGTTCCAG16610.11882516890200107No Hit
GTTTAAAATTGAACTTAAATTCATT16580.11861055390699445No Hit
TTGTAGAACAGTGTATATCAATGAG16570.11853901557532558No Hit
GCGTTGATACCACTGCTTCCCATGT16540.11832440058031896No Hit
ATTATAACCTAGACTTACAAGTCAA16350.11696517227861032No Hit
AGGGATAACAGCGCAATCCTATTTA16330.11682209561527257No Hit
ACCTTTGCACGGTCAGGATACCGCG16220.11603517396691496No Hit
GTCCTAAAGTGTGTATTTCTCATTT15890.11367440902184209No Hit
AAATATATCTGGGTCAATAAGATAT15860.11345979402683545No Hit
GGATTGCTCCGGTCTGAACTCAGAT15790.11295902570515333No Hit
CCTTTAGGCATTCCGGTGTTGGGTT15700.11231518072013347No Hit
ATTTAAGAGTTCATATCGACAATTA15220.10888134080002747No Hit
TATCAACGCAGAGTACATGGGAGAA14970.10709288250830559No Hit
GGGCAGGCAGTGCCTCTAATACTTG14950.10694980584496785No Hit
GTGTATATCAATGAGTTACAATGAA14850.10623442252827911No Hit
CTTAAATAGGATTGCGCTGTTATCC14590.10437442590488835No Hit
GTTTAGATTATAGCCAAAAGAGGGA14520.10387365758320623No Hit
CTGTAGGACGTGGAATATGGCAAGA14510.10380211925153734No Hit
CTATAGTATAAGTTTGAAATTTCGG14430.10322981259818637No Hit
ACTAAAAGTATTGGAGAAAGAAATT14420.10315827426651748No Hit
GTTATACGCGTATGCCTGGAGAATT14400.10301519760317973No Hit
TCTAGGAGCTATAGAACTAGTACCG14180.10144135430646449No Hit
GTCCTACAGTGGACATTTCTAAATT14140.10115520097978899No Hit
GTTATCCCTAGGGTAACTTGGTCCG14110.10094058598478237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTGA250.006023570419.00447817
GAACTGC350.002169230716.2866346
CTACACG551.1302223E-515.5424374
ACGGTTG508.6927685E-515.20412819
ATAGGAC456.782721E-414.7717343
TTGCTAA1900.014.5008210
AAGTCGT602.5665218E-514.2508067
AACTTGC2100.014.0246037
GCCTATC759.664509E-713.9341227
ACAACTG551.9554174E-413.82193519
TACACGG551.9613694E-413.8169825
TCTAGGG551.9667398E-413.81253053
TATAACG551.9691308E-413.8105532
TAAATTC3450.013.49593416
TGAACTT3100.013.48463310
TTGAACT3100.013.4846339
TCGTCTT7750.013.36348314
GATCGAA500.001498370213.30122911
CCGTCTC500.001498774913.3007539
TAGCCTA1001.0080839E-813.2988475
Produced by FastQC (version 0.11.5)