##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062681_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1397852
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.61867207687223	32.0	32.0	32.0	32.0	32.0
2	30.487410684392913	32.0	32.0	32.0	32.0	32.0
3	30.562927262685893	32.0	32.0	32.0	32.0	32.0
4	30.508550261401062	32.0	32.0	32.0	32.0	32.0
5	30.469036779287077	32.0	32.0	32.0	32.0	32.0
6	33.95921098943236	36.0	36.0	36.0	32.0	36.0
7	33.65401845116651	36.0	36.0	36.0	27.0	36.0
8	33.60955952418425	36.0	36.0	36.0	27.0	36.0
9	33.67943387425851	36.0	36.0	36.0	27.0	36.0
10	33.52460632456083	36.0	36.0	36.0	27.0	36.0
11	33.94511722271027	36.0	36.0	36.0	32.0	36.0
12	33.688898395538295	36.0	36.0	36.0	27.0	36.0
13	33.83805009400137	36.0	36.0	36.0	32.0	36.0
14	33.73809959852688	36.0	36.0	36.0	32.0	36.0
15	33.76371604433087	36.0	36.0	36.0	32.0	36.0
16	33.718119657875086	36.0	36.0	36.0	27.0	36.0
17	33.63220498307403	36.0	36.0	36.0	27.0	36.0
18	33.73170621782563	36.0	36.0	36.0	32.0	36.0
19	33.66337494956548	36.0	36.0	36.0	27.0	36.0
20	33.526961366439366	36.0	36.0	36.0	27.0	36.0
21	33.44049155418456	36.0	36.0	36.0	21.0	36.0
22	33.43885690330593	36.0	36.0	36.0	27.0	36.0
23	33.48511931162956	36.0	36.0	36.0	27.0	36.0
24	33.465441978120715	36.0	36.0	36.0	27.0	36.0
25	33.013185229909894	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	22.0
4	72.0
5	120.0
6	260.0
7	67.0
8	202.0
9	308.0
10	141.0
11	53.0
12	101.0
13	65.0
14	1223.0
15	1140.0
16	1278.0
17	1825.0
18	2503.0
19	3278.0
20	4993.0
21	6669.0
22	8953.0
23	11798.0
24	14636.0
25	18221.0
26	22407.0
27	26716.0
28	32837.0
29	41657.0
30	52309.0
31	69202.0
32	95792.0
33	134334.0
34	291407.0
35	553263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.907193541509564	21.81872555851433	13.593365223226868	21.680715676749234
2	12.95446545847892	23.24781298558588	41.95419074027969	21.843530815655505
3	17.261018995999336	29.93515375942216	31.773584743677063	21.030242500901437
4	9.624410794165037	19.811840497294018	40.9952749054838	29.568473803057145
5	9.771113972116883	41.826393565456485	35.796907394300426	12.60558506812621
6	25.62220194320683	41.423157442696876	20.34641329826257	12.608227315833737
7	24.237541002618727	37.1232090213636	22.343008829791348	16.29624114622633
8	25.751186668356535	34.78339946024401	21.427052386690985	18.038361484708464
9	26.202337756638716	17.446455949191783	22.614641090695304	33.73656520347419
10	14.864937459307184	28.66700904141517	34.28503485704422	22.183018642233428
11	29.69127384100553	26.361913411702687	25.930146605888876	18.01666614140291
12	22.63410126364652	29.76496461216654	30.80403449955586	16.796899624631077
13	26.015902610300095	23.96314651359515	29.185179242803105	20.83577163330165
14	21.527931980488084	25.418782725794554	29.088441124012405	23.964844169704957
15	23.021868189244028	29.65007889107016	29.02546788528243	18.302585034403382
16	20.872456207448277	30.730561034548455	28.7130024737832	19.68398028422007
17	17.927150202209333	29.403514703333194	30.845267957807515	21.82406713664996
18	18.753810082903104	28.48247845400357	33.98325318578448	18.78045827730885
19	20.268013779636142	30.800810617397328	31.276894323887355	17.65428127907918
20	20.16736839240102	28.80181823130733	33.1951848100613	17.835628566230348
21	22.331002598169174	27.75363310298191	29.286531426242295	20.628832872606615
22	19.776315365242905	30.32359758260103	29.799780041054223	20.100307011101837
23	20.068231169264738	29.9658342664612	30.31866764385393	19.647266920420133
24	19.127178061034066	32.34243779501386	29.994218820854368	18.53616532309771
25	20.56026630773099	29.735404329148217	30.909060952922644	18.795268410198148
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1293.0
1	1293.0
2	2401.0
3	3509.0
4	3509.0
5	3509.0
6	9378.5
7	15248.0
8	15248.0
9	15248.0
10	14606.0
11	13964.0
12	13964.0
13	13964.0
14	13179.5
15	12395.0
16	12395.0
17	12395.0
18	20640.0
19	28885.0
20	28885.0
21	28885.0
22	43676.0
23	58467.0
24	58467.0
25	58467.0
26	86158.0
27	113849.0
28	113849.0
29	113849.0
30	133261.0
31	152673.0
32	152673.0
33	152673.0
34	154431.5
35	156190.0
36	156190.0
37	156190.0
38	147702.5
39	139215.0
40	139215.0
41	139215.0
42	140526.5
43	141838.0
44	141838.0
45	141838.0
46	190571.0
47	239304.0
48	239304.0
49	239304.0
50	196033.5
51	152763.0
52	152763.0
53	152763.0
54	113843.5
55	74924.0
56	74924.0
57	74924.0
58	57013.5
59	39103.0
60	39103.0
61	39103.0
62	30980.5
63	22858.0
64	22858.0
65	22858.0
66	18697.0
67	14536.0
68	14536.0
69	14536.0
70	11239.0
71	7942.0
72	7942.0
73	7942.0
74	6379.0
75	4816.0
76	4816.0
77	4816.0
78	3445.0
79	2074.0
80	2074.0
81	2074.0
82	1458.0
83	842.0
84	842.0
85	842.0
86	562.5
87	283.0
88	283.0
89	283.0
90	187.0
91	91.0
92	91.0
93	91.0
94	80.5
95	70.0
96	70.0
97	70.0
98	395.0
99	720.0
100	720.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009013829790278228
2	0.00400614657345699
3	0.006009219860185484
4	0.014879972987125963
5	0.03712839413614603
6	0.048431450539828245
7	0.07082294835218607
8	0.09264213951119288
9	0.09908058936139161
10	0.12311746880213356
11	0.12361823712381569
12	0.13535052351751115
13	0.12368977545548455
14	0.12748130703393493
15	0.11610671229858383
16	0.11517671398688846
17	0.12690900038058392
18	0.134849755195829
19	0.13442052520581577
20	0.12662284705390844
21	0.13370514188912702
22	0.15287741477638547
23	0.1437205083227695
24	0.13899897843262377
25	0.13399129521580252
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1397852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.234341463517598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.43519341962808	14.252097547016623
2	13.50662010202238	5.465951263246634
3	5.373083832950136	3.2616243896405703
4	2.7150876826583796	2.1975204509720143
5	1.5396660829863582	1.5577064631471298
6	1.0267827059636248	1.2465763128781542
7	0.7309039300405196	1.0352551788226794
8	0.5141457725204164	0.8322720898561717
9	0.40970233829050445	0.7461051310234501
>10	2.8670730755558993	11.60355766541211
>50	0.38063067621037244	5.4265766229352455
>100	0.3777874717640435	16.519053291329815
>500	0.06788090979015207	9.499115438144537
>1k	0.051177230417706276	18.728631550226346
>5k	0.002843179467650349	3.729013884497929
>10k+	0.0014215897338251745	3.8989427208506164
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	16488	1.179524012556408	No Hit
GTACATGGAAGCAGTGGTATCAACG	14550	1.0408827257821285	No Hit
TCCATGTACTCTGCGTTGATACCAC	12622	0.9029568223245379	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10558	0.7553017057599803	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	9865	0.7057256419134501	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	6780	0.48502988871497127	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	6323	0.4523368711422954	No Hit
GAGTACATGGAAGCAGTGGTATCAA	6225	0.4453261146387458	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6107	0.4368845915018185	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	5764	0.4123469437393944	No Hit
GTACATGGGGTGGTATCAACGCAAA	5630	0.40276080729576524	No Hit
CATGTACTCTGCGTTGATACCACTG	5161	0.3692093297430629	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4194	0.300031763019261	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	4188	0.2996025330292477	No Hit
CCCATGTACTCTGCGTTGATACCAC	4181	0.2991017647075656	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3832	0.2741348869551283	No Hit
GCGTTGATACCACTGCTTCCATGTA	3693	0.2641910588531547	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3565	0.25503415239953875	No Hit
GTCAGGATACCGCGGCCGTTAAACT	3397	0.24301571267916774	No Hit
GTACATGGGTGGTATCAACGCAAAA	3391	0.2425864826891545	No Hit
CCATTGGGATGTCCTGATCCAACAT	3381	0.24187109937246573	No Hit
GTATCAACGCAGAGTACATGGGAAG	3366	0.24079802439743264	No Hit
CTCTAATACTTGTAATGCTAGAGGT	3319	0.2374357228089955	No Hit
GTATCAACGCAGAGTACATGGAAGC	3269	0.2338588062255518	No Hit
GTATCAACGCAGAGTACATGGGGTG	3216	0.23006727464710142	No Hit
CTGTTAGTATGAGTAACAAGAATTC	3013	0.2155449933183198	No Hit
ATCTATAACTTTATAGATGCAACAC	2857	0.20438501357797537	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	2722	0.19472733880267723	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	2721	0.1946558004710084	No Hit
CTTCTACACCATTGGGATGTCCTGA	2709	0.19379734049098188	No Hit
GTATCAACGCAGAGTACATGGGAGA	2698	0.19301041884262424	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2654	0.18986273224919376	No Hit
GATATATTTTGATCAACGGACCAAG	2645	0.1892188872641739	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2605	0.1863573539974189	No Hit
GTACATGGGAGTGGTATCAACGCAA	2510	0.17956121248887577	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2495	0.17848813751384268	No Hit
GTTATATAATTTAAGCTCCATAGGG	2488	0.17798736919216054	No Hit
CTATAGAACTAGTACCGCAAGGGAA	2460	0.17598429590543205	No Hit
GTTATAGATTAACCCAATTTTAAGT	2429	0.17376660762369694	No Hit
ATAAATAATCCACCTATAACTTCTC	2405	0.17204968766364395	No Hit
CCTCTAGCATTACAAGTATTAGAGG	2403	0.1719066110003062	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	2380	0.17026122937192206	No Hit
ACCTATAACTTCTCTGTTAACCCAA	2378	0.1701181527085843	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	2364	0.16911661606522008	No Hit
CAGTGGTATCAACGCAGAGTACATG	2319	0.1658973911401207	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	2313	0.16546816115010746	No Hit
ATCGTAAATAGATAGAAACCGACCT	2286	0.16353662619504783	No Hit
CCTATAACTTCTCTGTTAACCCAAC	2249	0.16088970792329946	No Hit
CACTATAAATAATCCACCTATAACT	2230	0.15953047962159084	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	2207	0.1578850979932067	No Hit
GATTAAAGATAAGAGACAGTTGGAC	2205	0.15774202132986898	No Hit
GATTAAACCTTGTACCTTTTGCATA	2200	0.1573843296715246	No Hit
GTACATGGGAGAAATCGTAAATAGA	2164	0.1548089497314451	No Hit
CTGTTAACCCAACACCGGAATGCCT	2139	0.15302049143972324	No Hit
GACTATAGGCAATAATCACACTATA	2061	0.147440501569551	No Hit
TATCAACGCAGAGTACATGGGGTGG	2045	0.146295888262849	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2001	0.1431482016694185	No Hit
TATCAACGCAGAGTACATGGAAGCA	1962	0.14035820673433239	No Hit
GTGTAGAAGCTATTAATGGTTCGTT	1960	0.14021513007099465	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	1959	0.14014359173932575	No Hit
GATAGAAACCGACCTGGATTGCTCC	1941	0.138855901769286	No Hit
GTTAGTATGAGTAACAAGAATTCCA	1940	0.13878436343761716	No Hit
GTCCTGATCCAACATCGAGGTCGTA	1902	0.13606590683419992	No Hit
AGCATGAACGGCTAAACGAGGGTCC	1887	0.13499283185916677	No Hit
GACCTGGATTGCTCCGGTCTGAACT	1871	0.13384821855246476	No Hit
TATCAACGCAGAGTACATGGGAAGC	1850	0.1323459135874184	No Hit
GGTATCAACGCAGAGTACATGGGAA	1830	0.1309151469540409	No Hit
TACTAACAGTGTTGCATCTATAAAG	1829	0.13084360862237204	No Hit
GCTATCACCAAGCTCGTTAGGCTTT	1801	0.12884053533564355	No Hit
CTTTATTGGTGGCTGCTTTTAGGCC	1790	0.12805361368728593	No Hit
CCTATAGTCTGATTAACTAACAATG	1777	0.12712361537559053	No Hit
GTATCAACGCAGAGTACATGGGAGT	1709	0.12225900882210707	No Hit
GGTATCAACGCAGAGTACATGGGGT	1704	0.12190131716376268	No Hit
GGTATCAACGCAGAGTACATGGGAG	1681	0.12025593553537857	No Hit
GATATACACTGTTCTACAAATCCCG	1664	0.1190397838970077	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	1661	0.11882516890200107	No Hit
GTTTAAAATTGAACTTAAATTCATT	1658	0.11861055390699445	No Hit
TTGTAGAACAGTGTATATCAATGAG	1657	0.11853901557532558	No Hit
GCGTTGATACCACTGCTTCCCATGT	1654	0.11832440058031896	No Hit
ATTATAACCTAGACTTACAAGTCAA	1635	0.11696517227861032	No Hit
AGGGATAACAGCGCAATCCTATTTA	1633	0.11682209561527257	No Hit
ACCTTTGCACGGTCAGGATACCGCG	1622	0.11603517396691496	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1589	0.11367440902184209	No Hit
AAATATATCTGGGTCAATAAGATAT	1586	0.11345979402683545	No Hit
GGATTGCTCCGGTCTGAACTCAGAT	1579	0.11295902570515333	No Hit
CCTTTAGGCATTCCGGTGTTGGGTT	1570	0.11231518072013347	No Hit
ATTTAAGAGTTCATATCGACAATTA	1522	0.10888134080002747	No Hit
TATCAACGCAGAGTACATGGGAGAA	1497	0.10709288250830559	No Hit
GGGCAGGCAGTGCCTCTAATACTTG	1495	0.10694980584496785	No Hit
GTGTATATCAATGAGTTACAATGAA	1485	0.10623442252827911	No Hit
CTTAAATAGGATTGCGCTGTTATCC	1459	0.10437442590488835	No Hit
GTTTAGATTATAGCCAAAAGAGGGA	1452	0.10387365758320623	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1451	0.10380211925153734	No Hit
CTATAGTATAAGTTTGAAATTTCGG	1443	0.10322981259818637	No Hit
ACTAAAAGTATTGGAGAAAGAAATT	1442	0.10315827426651748	No Hit
GTTATACGCGTATGCCTGGAGAATT	1440	0.10301519760317973	No Hit
TCTAGGAGCTATAGAACTAGTACCG	1418	0.10144135430646449	No Hit
GTCCTACAGTGGACATTTCTAAATT	1414	0.10115520097978899	No Hit
GTTATCCCTAGGGTAACTTGGTCCG	1411	0.10094058598478237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.4307666333774962E-4	0.0
3	0.0	0.0	0.0	1.4307666333774962E-4	0.0
4	0.0	0.0	0.0	2.1461499500662445E-4	0.0
5	0.0	0.0	0.0	2.1461499500662445E-4	0.0
6	0.0	0.0	0.0	2.1461499500662445E-4	0.0
7	0.0	0.0	0.0	3.5769165834437407E-4	0.0
8	0.0	0.0	2.1461499500662445E-4	3.5769165834437407E-4	0.0
9	0.0	0.0	2.1461499500662445E-4	9.299983116953727E-4	0.0
10	0.0	0.0	2.1461499500662445E-4	0.001144613306701997	0.0
11	0.0	0.0	2.1461499500662445E-4	0.001216151638370872	0.0
12	0.0	0.0	2.1461499500662445E-4	0.001216151638370872	0.0
13	0.0	0.0	2.1461499500662445E-4	0.001216151638370872	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTGA	25	0.0060235704	19.004478	17
GAACTGC	35	0.0021692307	16.286634	6
CTACACG	55	1.1302223E-5	15.542437	4
ACGGTTG	50	8.6927685E-5	15.204128	19
ATAGGAC	45	6.782721E-4	14.771734	3
TTGCTAA	190	0.0	14.50082	10
AAGTCGT	60	2.5665218E-5	14.250806	7
AACTTGC	210	0.0	14.024603	7
GCCTATC	75	9.664509E-7	13.934122	7
ACAACTG	55	1.9554174E-4	13.821935	19
TACACGG	55	1.9613694E-4	13.816982	5
TCTAGGG	55	1.9667398E-4	13.8125305	3
TATAACG	55	1.9691308E-4	13.810553	2
TAAATTC	345	0.0	13.495934	16
TGAACTT	310	0.0	13.484633	10
TTGAACT	310	0.0	13.484633	9
TCGTCTT	775	0.0	13.363483	14
GATCGAA	50	0.0014983702	13.301229	11
CCGTCTC	50	0.0014987749	13.300753	9
TAGCCTA	100	1.0080839E-8	13.298847	5
>>END_MODULE