FastQCFastQC Report
Thu 2 Feb 2017
SRR4062680_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062680_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541055
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG80081.4800713421001561No Hit
TCCATGTACTCTGCGTTGATACCAC62691.1586622432100249No Hit
GAGTACATGGAAGCAGTGGTATCAA35690.659637190304128No Hit
CATGTACTCTGCGTTGATACCACTG27740.5127020358373918No Hit
GCTTCCATGTACTCTGCGTTGATAC20500.3788893920211439No Hit
GTACATGGGAAGCAGTGGTATCAAC18260.33748879503932133No Hit
CCCATGTACTCTGCGTTGATACCAC17960.3319440722292558No Hit
CATGGAAGCAGTGGTATCAACGCAG17580.3249207566698395No Hit
GCGTTGATACCACTGCTTCCATGTA17490.3232573398268198No Hit
GTATCAACGCAGAGTACTTTTTTTT17480.323072515733151No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14710.27187624178687936No Hit
GTATCAACGCAGAGTACATGGAAGC13250.24489192411122715No Hit
TATCAACGCAGAGTACTTTTTTTTT11550.21347182818752253No Hit
ACTCTGCGTTGATACCACTGCTTCC11200.20700298490911273No Hit
ACGCAGAGTACATGGAAGCAGTGGT11150.20607886444076848No Hit
CAGTGGTATCAACGCAGAGTACATG11110.20533956806609308No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9300.17188640711203113No Hit
TATCAACGCAGAGTACATGGAAGCA8040.14859857130975593No Hit
GTATCAACGCAGAGTACATGGGAAG7190.13288852334790363No Hit
GCGCAAGACGGACCAGAGCGAAAGC7020.12974651375553317No Hit
GGTATCAACGCAGAGTACTTTTTTT6660.12309284638345455No Hit
GTCTTGCGCCGGTCCAAGAATTTCA6570.12142942954043488No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG6440.11902671632273983No Hit
GAATAGGACCGCGGTTCTATTTTGT6420.11865706813540212No Hit
GAGTACATGGGAAGCAGTGGTATCA6310.11662400310504478No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6210.11477576216835626No Hit
AAAAAGTACTCTGCGTTGATACCAC5940.1097855116392973No Hit
GGTATCAACGCAGAGTACATGGAAG5900.1090462152646219No Hit
AAGCAGTGGTATCAACGCAGAGTAC5880.1086765670772842No Hit
GAACTACGACGGTATCTGATCGTCT5760.10645867795325799No Hit
GAATAACGCCGCCGCATCGCCAGTC5610.10368631654822523No Hit
GCGTTGATACCACTGCTTCCCATGT5500.10165325151786787No Hit
ATACCACTGCTTCCATGTACTCTGC5480.10128360333053017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG402.7590885E-416.62335
TCGCCAG704.402409E-714.93395417
TGTAGAA1850.014.8862352
TCTTATA456.771713E-414.7721642
ACGCCCC801.277458E-714.25513817
TCCAACG801.277458E-714.25513818
TCGGCGT602.5623825E-514.24986213
CTAAGGT400.00527678714.2485434
CGTTATT400.005286515714.2445882
TAAGAGC759.670421E-713.929333
GGCGGTC551.9504533E-413.82316418
GCATCGA551.9565909E-413.8180478
ATCCTAG551.9673724E-413.8091011
GGTTCTA1051.3624231E-913.57129613
GTATTAA500.001504777713.291261
GTCGGCG655.4401553E-513.15371812
CGCCAGT801.9844738E-613.0672118
GAGCATC801.9927447E-613.0623726
AAATGTC801.9927447E-613.0623727
ATCGCCA905.371676E-712.66888916
Produced by FastQC (version 0.11.5)