##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062680_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541055
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.123723096542864	32.0	32.0	32.0	32.0	32.0
2	30.816552845828983	32.0	32.0	32.0	32.0	32.0
3	30.79486558667788	32.0	32.0	32.0	32.0	32.0
4	30.86820193880474	32.0	32.0	32.0	32.0	32.0
5	30.667714003197457	32.0	32.0	32.0	32.0	32.0
6	34.310739203962626	36.0	36.0	36.0	32.0	36.0
7	34.17210080306069	36.0	36.0	36.0	32.0	36.0
8	34.16393342636146	36.0	36.0	36.0	32.0	36.0
9	34.31878644500097	36.0	36.0	36.0	32.0	36.0
10	33.994383195793404	36.0	36.0	36.0	32.0	36.0
11	34.33711545037011	36.0	36.0	36.0	32.0	36.0
12	34.11588470673037	36.0	36.0	36.0	32.0	36.0
13	34.218868691722655	36.0	36.0	36.0	32.0	36.0
14	34.109881620168004	36.0	36.0	36.0	32.0	36.0
15	34.055508220051564	36.0	36.0	36.0	32.0	36.0
16	34.05323673194037	36.0	36.0	36.0	32.0	36.0
17	33.94634371736699	36.0	36.0	36.0	32.0	36.0
18	33.95977858073579	36.0	36.0	36.0	32.0	36.0
19	33.9578397759932	36.0	36.0	36.0	32.0	36.0
20	33.9488776556912	36.0	36.0	36.0	32.0	36.0
21	33.86816867046788	36.0	36.0	36.0	32.0	36.0
22	33.81611666096792	36.0	36.0	36.0	32.0	36.0
23	33.72617016754304	36.0	36.0	36.0	27.0	36.0
24	33.68104721331473	36.0	36.0	36.0	27.0	36.0
25	33.13138035874356	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	28.0
5	47.0
6	100.0
7	10.0
8	87.0
9	99.0
10	72.0
11	20.0
12	23.0
13	24.0
14	88.0
15	111.0
16	168.0
17	242.0
18	293.0
19	495.0
20	721.0
21	1081.0
22	1690.0
23	2511.0
24	3476.0
25	4713.0
26	6481.0
27	8655.0
28	11514.0
29	15706.0
30	20268.0
31	28419.0
32	40873.0
33	57822.0
34	122470.0
35	212745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.500716246314795	16.324316331340167	11.226675785329428	24.94829163701561
2	15.595109373700408	20.397201659781715	37.023852430988754	26.983836535529125
3	20.397914313862124	24.881474101744665	27.196726189926085	27.52388539446713
4	12.50727865943268	15.412457367851895	34.65769504496594	37.42256892774948
5	13.910932461615833	36.985807472709524	33.729994379197116	15.37326568647753
6	34.735006638239966	33.57969401227057	17.445257633975956	14.24004171551351
7	29.767519280918826	30.09062494220348	21.1257837208012	19.016072056076496
8	28.913168551464892	32.46786161578976	20.178594883387937	18.440374949357416
9	27.139276100972165	15.075525626913578	18.032911228482366	39.75228704363189
10	16.0040338995596	27.067836127456424	31.708115909847894	25.22001406313608
11	37.60094000962215	21.229229118093336	20.737389437844637	20.43244143443988
12	24.017522203489264	23.742141565635162	29.012682684887192	23.22765354598838
13	31.090398365679267	19.45844621101094	24.647118473449687	24.804036949860105
14	24.30453907035013	19.478272455250494	24.219786564322156	31.997401910077222
15	25.955092562610442	26.293332346492242	24.130855128643987	23.62071996225333
16	26.41677351791308	24.911049806557614	25.19006570195256	23.48211097357675
17	24.006180721330892	25.271193027258093	26.929624900534797	23.79300135087622
18	25.85602558360754	23.022285720630855	28.592235345500843	22.52945335026076
19	24.77588682932607	27.228177188203244	24.692052587942676	23.30388339452801
20	24.308435340161548	23.385423895041683	28.31135907328763	23.994781691509147
21	25.484531808937263	25.3684995567827	23.383737348875858	25.76323128540418
22	24.227532119448625	25.936558883033562	24.7117532841338	25.12415571338401
23	22.85806295193377	25.272016242317008	25.036413808930686	26.833506996818535
24	23.310107431363296	26.98738768749595	24.998103063783322	24.70440181735743
25	25.470666289134286	24.50055612000348	24.23184189536061	25.796935695501627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	97.0
2	222.5
3	348.0
4	348.0
5	348.0
6	1195.5
7	2043.0
8	2043.0
9	2043.0
10	1920.0
11	1797.0
12	1797.0
13	1797.0
14	1543.0
15	1289.0
16	1289.0
17	1289.0
18	2069.0
19	2849.0
20	2849.0
21	2849.0
22	4876.0
23	6903.0
24	6903.0
25	6903.0
26	10777.0
27	14651.0
28	14651.0
29	14651.0
30	18942.0
31	23233.0
32	23233.0
33	23233.0
34	27106.5
35	30980.0
36	30980.0
37	30980.0
38	34834.5
39	38689.0
40	38689.0
41	38689.0
42	47098.0
43	55507.0
44	55507.0
45	55507.0
46	75882.5
47	96258.0
48	96258.0
49	96258.0
50	84803.5
51	73349.0
52	73349.0
53	73349.0
54	64482.0
55	55615.0
56	55615.0
57	55615.0
58	51590.0
59	47565.0
60	47565.0
61	47565.0
62	42620.5
63	37676.0
64	37676.0
65	37676.0
66	31451.5
67	25227.0
68	25227.0
69	25227.0
70	18880.0
71	12533.0
72	12533.0
73	12533.0
74	9707.5
75	6882.0
76	6882.0
77	6882.0
78	5670.0
79	4458.0
80	4458.0
81	4458.0
82	3114.0
83	1770.0
84	1770.0
85	1770.0
86	1264.0
87	758.0
88	758.0
89	758.0
90	510.5
91	263.0
92	263.0
93	263.0
94	157.5
95	52.0
96	52.0
97	52.0
98	157.5
99	263.0
100	263.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0073929637467540265
2	0.0036964818733770132
3	0.006284019184740923
4	0.01663416843019656
5	0.038258587389452085
6	0.046021199323543815
7	0.0674607941891305
8	0.09278169502176305
9	0.09426028777111384
10	0.11736329947972018
11	0.11736329947972018
12	0.13159475469222168
13	0.11958118860374639
14	0.12161425363410375
15	0.11274269713799892
16	0.10793727070260879
17	0.12290802228978569
18	0.13104028241121513
19	0.12993133784920202
20	0.12013566088475294
21	0.12715897644416926
22	0.14896821949709366
23	0.1364001811276118
24	0.13122510650488398
25	0.13011616194287087
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	541055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.82154706621727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.50693999619979	38.8165181311538
2	12.410128944018945	12.117633886764922
3	3.484883240015119	5.104121733680094
4	1.4179958897231693	2.7691501227928934
5	0.7338237214342167	1.7913204677153658
6	0.4545733188051065	1.3315783607454055
7	0.29557056918205077	1.0101148718297082
8	0.2092337022329247	0.8172090433122898
9	0.16156001536816011	0.7098848904883873
>10	1.0616595782404088	10.175736202865227
>50	0.13560331449650687	4.806236032746352
>100	0.11477040300573789	11.131087310448015
>500	0.007196823950854852	2.2361295148514317
>1k	0.005302922911156207	4.543085824624143
>5k	7.575604158794582E-4	2.640193605981963
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8008	1.4800713421001561	No Hit
TCCATGTACTCTGCGTTGATACCAC	6269	1.1586622432100249	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3569	0.659637190304128	No Hit
CATGTACTCTGCGTTGATACCACTG	2774	0.5127020358373918	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2050	0.3788893920211439	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1826	0.33748879503932133	No Hit
CCCATGTACTCTGCGTTGATACCAC	1796	0.3319440722292558	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1758	0.3249207566698395	No Hit
GCGTTGATACCACTGCTTCCATGTA	1749	0.3232573398268198	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1748	0.323072515733151	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1471	0.27187624178687936	No Hit
GTATCAACGCAGAGTACATGGAAGC	1325	0.24489192411122715	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1155	0.21347182818752253	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1120	0.20700298490911273	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1115	0.20607886444076848	No Hit
CAGTGGTATCAACGCAGAGTACATG	1111	0.20533956806609308	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	930	0.17188640711203113	No Hit
TATCAACGCAGAGTACATGGAAGCA	804	0.14859857130975593	No Hit
GTATCAACGCAGAGTACATGGGAAG	719	0.13288852334790363	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	702	0.12974651375553317	No Hit
GGTATCAACGCAGAGTACTTTTTTT	666	0.12309284638345455	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	657	0.12142942954043488	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	644	0.11902671632273983	No Hit
GAATAGGACCGCGGTTCTATTTTGT	642	0.11865706813540212	No Hit
GAGTACATGGGAAGCAGTGGTATCA	631	0.11662400310504478	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	621	0.11477576216835626	No Hit
AAAAAGTACTCTGCGTTGATACCAC	594	0.1097855116392973	No Hit
GGTATCAACGCAGAGTACATGGAAG	590	0.1090462152646219	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	588	0.1086765670772842	No Hit
GAACTACGACGGTATCTGATCGTCT	576	0.10645867795325799	No Hit
GAATAACGCCGCCGCATCGCCAGTC	561	0.10368631654822523	No Hit
GCGTTGATACCACTGCTTCCCATGT	550	0.10165325151786787	No Hit
ATACCACTGCTTCCATGTACTCTGC	548	0.10128360333053017	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	1.8482409366885066E-4
8	0.0	0.0	0.0	0.0	1.8482409366885066E-4
9	0.0	0.0	0.0	0.0	1.8482409366885066E-4
10	0.0	0.0	0.0	0.0	1.8482409366885066E-4
11	0.0	0.0	0.0	1.8482409366885066E-4	1.8482409366885066E-4
12	0.0	0.0	0.0	3.696481873377013E-4	1.8482409366885066E-4
13	0.0	0.0	0.0	3.696481873377013E-4	1.8482409366885066E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACAG	40	2.7590885E-4	16.6233	5
TCGCCAG	70	4.402409E-7	14.933954	17
TGTAGAA	185	0.0	14.886235	2
TCTTATA	45	6.771713E-4	14.772164	2
ACGCCCC	80	1.277458E-7	14.255138	17
TCCAACG	80	1.277458E-7	14.255138	18
TCGGCGT	60	2.5623825E-5	14.249862	13
CTAAGGT	40	0.005276787	14.248543	4
CGTTATT	40	0.0052865157	14.244588	2
TAAGAGC	75	9.670421E-7	13.92933	3
GGCGGTC	55	1.9504533E-4	13.823164	18
GCATCGA	55	1.9565909E-4	13.818047	8
ATCCTAG	55	1.9673724E-4	13.809101	1
GGTTCTA	105	1.3624231E-9	13.571296	13
GTATTAA	50	0.0015047777	13.29126	1
GTCGGCG	65	5.4401553E-5	13.153718	12
CGCCAGT	80	1.9844738E-6	13.06721	18
GAGCATC	80	1.9927447E-6	13.062372	6
AAATGTC	80	1.9927447E-6	13.062372	7
ATCGCCA	90	5.371676E-7	12.668889	16
>>END_MODULE