FastQCFastQC Report
Thu 2 Feb 2017
SRR4062680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541055
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24590.45448244633170376No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21830.403470996479101No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC9460.17484359261073273No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA9380.17336499986138193No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC6850.1266045041631627No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6040.1116337525759858No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC5970.11033998392030386No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG5970.11033998392030386No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT5710.10553455748491374No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5650.10442561292290063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAA300.005742245429.3333321
GTCCTAC3100.024.838711
GCATTCG1800.024.44444322
GAGCGAA1450.022.7586216
TGGACTA602.8699386E-422.05
TGAACCG500.002578612922.05
CTAAGAC500.002578612922.03
CGGGGGC2000.022.017
AGCGAAA1407.2759576E-1222.017
GTATAGA904.0731902E-721.9999981
GGCGAGA2050.021.46341519
TCCTACA3600.021.3888872
CCTACAG3550.021.0704213
ATTCGTA2100.020.95238124
GCGAGAA2000.020.920
CGCAATA1900.020.84210636
AATCGCT956.8518784E-720.84210615
CGAATGC1800.020.77777743
ATACGAA1800.020.77777740
TAACGGC755.4654767E-520.53333336
Produced by FastQC (version 0.11.5)