##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062680_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541055
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11590688562161	32.0	32.0	32.0	32.0	32.0
2	31.338244725582427	32.0	32.0	32.0	32.0	32.0
3	31.461912374897192	32.0	32.0	32.0	32.0	32.0
4	31.59115431887701	32.0	32.0	32.0	32.0	32.0
5	31.506113056898098	32.0	32.0	32.0	32.0	32.0
6	35.11910988716489	36.0	36.0	36.0	36.0	36.0
7	35.1364500836329	36.0	36.0	36.0	36.0	36.0
8	35.074153274620876	36.0	36.0	36.0	36.0	36.0
9	35.19158865549713	36.0	36.0	36.0	36.0	36.0
10	35.06738501631072	36.0	36.0	36.0	36.0	36.0
11	35.20102392547893	36.0	36.0	36.0	36.0	36.0
12	35.10296919906479	36.0	36.0	36.0	36.0	36.0
13	35.13357052425354	36.0	36.0	36.0	36.0	36.0
14	35.08573435233017	36.0	36.0	36.0	36.0	36.0
15	35.05190969494783	36.0	36.0	36.0	36.0	36.0
16	35.04325992736413	36.0	36.0	36.0	36.0	36.0
17	35.01237397307113	36.0	36.0	36.0	36.0	36.0
18	35.03184888782101	36.0	36.0	36.0	36.0	36.0
19	34.987134394839714	36.0	36.0	36.0	36.0	36.0
20	34.99018953710806	36.0	36.0	36.0	36.0	36.0
21	34.93440408091599	36.0	36.0	36.0	32.0	36.0
22	34.895156684625405	36.0	36.0	36.0	32.0	36.0
23	34.81644195137278	36.0	36.0	36.0	32.0	36.0
24	34.77315245215366	36.0	36.0	36.0	32.0	36.0
25	34.74640101283603	36.0	36.0	36.0	32.0	36.0
26	34.65695724094593	36.0	36.0	36.0	32.0	36.0
27	34.615425418857605	36.0	36.0	36.0	32.0	36.0
28	34.540745395569765	36.0	36.0	36.0	32.0	36.0
29	34.50733289591631	36.0	36.0	36.0	32.0	36.0
30	34.48092892589478	36.0	36.0	36.0	32.0	36.0
31	34.462087957786174	36.0	36.0	36.0	32.0	36.0
32	34.39018584062618	36.0	36.0	36.0	32.0	36.0
33	34.35873986932937	36.0	36.0	36.0	32.0	36.0
34	34.315352413340605	36.0	36.0	36.0	32.0	36.0
35	34.2344271839277	36.0	36.0	36.0	32.0	36.0
36	34.16822689005739	36.0	36.0	36.0	32.0	36.0
37	34.07762057461811	36.0	36.0	36.0	32.0	36.0
38	33.998645239393404	36.0	36.0	36.0	32.0	36.0
39	33.80907116651727	36.0	36.0	36.0	32.0	36.0
40	33.70685604975465	36.0	36.0	36.0	27.0	36.0
41	33.71365387991979	36.0	36.0	36.0	32.0	36.0
42	33.498361534409625	36.0	36.0	36.0	21.0	36.0
43	33.579767306466074	36.0	36.0	36.0	27.0	36.0
44	33.556656901793716	36.0	36.0	36.0	21.0	36.0
45	33.313609522137305	36.0	36.0	36.0	14.0	36.0
46	33.497167570764525	36.0	36.0	36.0	21.0	36.0
47	33.36795889512157	36.0	36.0	36.0	21.0	36.0
48	33.38888837548863	36.0	36.0	36.0	21.0	36.0
49	33.465547864819655	36.0	36.0	36.0	21.0	36.0
50	32.84045614586317	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	12.0
22	68.0
23	243.0
24	634.0
25	1559.0
26	3431.0
27	6343.0
28	10159.0
29	13628.0
30	17895.0
31	24016.0
32	34371.0
33	45978.0
34	94281.0
35	288435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.622424779055514	17.095492751024544	11.744763601880317	25.537318868039627
2	15.065328249269458	20.84848171197164	36.40287078332905	27.68331925542985
3	19.836910315459512	24.93808358531158	27.669244360985633	27.555761738243273
4	12.41611296448605	15.887294267680735	34.7702174455462	36.926375322287015
5	13.769210154235706	37.17939950651967	33.48347210542366	15.56791823382096
6	35.14258307411659	33.673780572328404	17.136953311413112	14.046683042141897
7	29.749655765125542	30.134644352237753	21.11947953535223	18.996220347284474
8	29.03641958765745	32.568777665856516	20.033083512766726	18.361719233719306
9	27.083013741671362	14.65618097975252	17.971001099703358	40.28980417887276
10	15.954015765495189	26.992819583960966	31.73651477206569	25.31664987847816
11	37.736644148931255	20.956464684736304	20.79455877868239	20.512332387650055
12	23.965031281477852	23.726977848832373	29.05102069105729	23.256970178632486
13	31.547809372429793	19.23113177033758	24.588997421703894	24.632061435528733
14	24.237831643733077	19.395994861890195	24.168707432700927	32.1974660616758
15	26.211013667741728	26.28937908345732	23.75137462919666	23.74823261960429
16	26.436683886111396	24.97084399922374	25.052536248625373	23.539935866039496
17	23.863747678147323	25.6308508377152	26.610973006441117	23.89442847769635
18	26.002162441895926	23.089704373862176	28.266257589339343	22.64187559490255
19	25.138479452181382	27.248800953692324	24.49455230983911	23.118167284287182
20	24.72262524142647	23.41388583415734	27.896609402001644	23.96687952241454
21	25.638798273742964	25.582796573361303	23.090258846143183	25.688146306752547
22	24.457217842918002	25.91048969143618	24.404173328035043	25.228119137610776
23	22.91190359575274	24.833889345815123	24.89672953766253	27.357477520769606
24	23.62144329134746	27.056214248089383	24.735008455702285	24.58733400486087
25	25.821958950568796	24.337636654314256	23.89812495956973	25.942279435547217
26	23.189509384443355	25.0974485033869	25.83563593350029	25.87740617866945
27	25.92324255389933	25.554888135217308	25.059744388278453	23.46212492260491
28	24.28274389849461	23.810703163264364	25.551930949718603	26.354621988522425
29	23.217048174400016	25.770762676622528	26.466625389285745	24.545563759691714
30	25.339752890186766	24.358706600992505	26.406003086562365	23.895537422258364
31	23.87557642014213	24.999861381929747	24.963266211383317	26.161295986544808
32	23.798504773082218	25.42754433467947	25.465248449787914	25.308702442450397
33	23.19505410725342	25.065289111088525	25.917328182901922	25.822328598756133
34	23.37193076489451	26.136159909805844	27.04493997837558	23.446969346924064
35	25.626784707654487	25.028324292354753	26.0479988171258	23.29689218286496
36	23.905333099222815	26.065187457837002	25.669848721479333	24.35963072146085
37	25.370618513829463	25.59481013944978	25.550637181062925	23.483934165657836
38	24.281265305745254	25.606269233257244	24.43503895167774	25.677426509319755
39	24.42542809880696	24.77936623818281	25.863729195737957	24.931476467272272
40	25.76152147193908	25.363780022363713	24.655164447237343	24.21953405845986
41	23.25308887266544	25.071203482085924	26.65588526120265	25.019822384045987
42	26.42152831043055	25.834896637125617	25.47596824722071	22.267606805223128
43	24.766243727532324	23.788339447930433	25.153450203768564	26.29196662076868
44	23.99571208102688	24.884715971574053	26.94476531960706	24.174806627791998
45	24.708948258494978	25.320900832632542	25.490754174714215	24.479396734158264
46	23.590436444421446	24.632476610467716	26.991760526675712	24.785326418435126
47	24.964005507757992	23.98295921856373	27.197974327933387	23.85506094574489
48	24.34003936753195	27.811775143007644	23.896091894539374	23.952093594921035
49	23.35640554102633	25.07508478805297	27.4583914759128	24.1101181950079
50	23.113546681945458	28.066093095895983	23.60203676151223	25.21832346064633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	17.0
2	13.0
3	70.0
4	127.0
5	137.5
6	148.0
7	134.5
8	121.0
9	161.0
10	201.0
11	289.0
12	377.0
13	645.0
14	913.0
15	1229.0
16	1545.0
17	1720.5
18	1896.0
19	2077.5
20	2259.0
21	2628.5
22	2998.0
23	3164.0
24	3330.0
25	3503.0
26	3676.0
27	4607.0
28	5538.0
29	6428.5
30	7319.0
31	8004.5
32	8690.0
33	10166.5
34	11643.0
35	13402.5
36	15162.0
37	17619.5
38	20077.0
39	20967.0
40	21857.0
41	23964.0
42	26071.0
43	27671.0
44	29271.0
45	34907.0
46	40543.0
47	45110.5
48	49678.0
49	47759.0
50	45840.0
51	40867.0
52	35894.0
53	34282.5
54	32671.0
55	33586.5
56	34502.0
57	33630.5
58	32759.0
59	30633.0
60	28507.0
61	25408.5
62	22310.0
63	19398.5
64	16487.0
65	14086.0
66	11685.0
67	10118.5
68	8552.0
69	8020.0
70	7488.0
71	5620.0
72	3752.0
73	3382.0
74	3012.0
75	2113.0
76	1214.0
77	1125.5
78	1037.0
79	897.5
80	758.0
81	590.0
82	422.0
83	380.5
84	339.0
85	275.0
86	211.0
87	152.0
88	93.0
89	59.5
90	26.0
91	17.0
92	8.0
93	7.5
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015155575680845756
2	0.003326833686039312
3	5.544722810065521E-4
4	0.0
5	0.0
6	3.696481873377013E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.8482409366885066E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	541055.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.92715627037259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.15691890603128	46.20032604985599
2	11.581568737786204	13.186115467840342
3	3.184323182642833	5.438233903010363
4	1.2577077351200745	2.8639089911854745
5	0.6518830234467549	1.8554923372878187
6	0.41019334188735795	1.40106842848128
7	0.2738585150434884	1.0912990537296994
8	0.1921836518373981	0.8752375024606753
9	0.1409318754001415	0.722056580494248
>10	0.9492915640704783	10.304347187704685
>50	0.10935738072812011	4.409105807747841
>100	0.08624912337425668	9.099466401557873
>500	0.004882025851373014	1.6932729973089544
>1k	6.509367801830685E-4	0.8600692913347375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2459	0.45448244633170376	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2183	0.403470996479101	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	946	0.17484359261073273	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	938	0.17336499986138193	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	685	0.1266045041631627	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	604	0.1116337525759858	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	597	0.11033998392030386	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	597	0.11033998392030386	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	571	0.10553455748491374	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	565	0.10442561292290063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.696481873377013E-4
2	0.0	0.0	0.0	0.0	3.696481873377013E-4
3	0.0	0.0	0.0	0.0	3.696481873377013E-4
4	0.0	0.0	0.0	1.8482409366885066E-4	3.696481873377013E-4
5	0.0	0.0	0.0	1.8482409366885066E-4	3.696481873377013E-4
6	0.0	0.0	0.0	1.8482409366885066E-4	3.696481873377013E-4
7	0.0	0.0	0.0	1.8482409366885066E-4	3.696481873377013E-4
8	0.0	0.0	0.0	3.696481873377013E-4	3.696481873377013E-4
9	0.0	0.0	0.0	3.696481873377013E-4	3.696481873377013E-4
10	0.0	0.0	0.0	3.696481873377013E-4	3.696481873377013E-4
11	0.0	0.0	0.0	5.54472281006552E-4	3.696481873377013E-4
12	0.0	0.0	0.0	7.392963746754026E-4	9.241204683442533E-4
13	0.0	0.0	0.0	9.241204683442533E-4	9.241204683442533E-4
14	0.0	0.0	0.0	9.241204683442533E-4	9.241204683442533E-4
15	0.0	0.0	0.0	0.0012937686556819547	9.241204683442533E-4
16	0.0	0.0	0.0	0.0020330650303573574	9.241204683442533E-4
17	0.0	0.0	0.0	0.002217889124026208	9.241204683442533E-4
18	0.0	0.0	0.0	0.00277236140503276	0.001108944562013104
19	0.0	0.0	0.0	0.00277236140503276	0.001108944562013104
20	0.0	0.0	0.0	0.0035116577797081627	0.001108944562013104
21	0.0	0.0	0.0	0.0053598987163966695	0.001108944562013104
22	0.0	0.0	0.0	0.0077626119340917284	0.0012937686556819547
23	0.0	0.0	0.0	0.012383214275812996	0.0012937686556819547
24	0.0	0.0	0.0	0.01922170574156047	0.0012937686556819547
25	0.0	0.0	0.0	0.021254770771917827	0.0012937686556819547
26	0.0	0.0	0.0	0.0273539658629899	0.0012937686556819547
27	0.0	0.0	0.0	0.034562105516075074	0.0012937686556819547
28	0.0	0.0	0.0	0.049902505290589684	0.0012937686556819547
29	0.0	0.0	0.0	0.06653667372078624	0.0012937686556819547
30	0.0	0.0	0.0	0.09037898180406798	0.0012937686556819547
31	0.0	0.0	0.0	0.13418229200358558	0.001663416843019656
32	0.0	0.0	0.0	0.19277152969661124	0.001663416843019656
33	0.0	0.0	0.0	0.2521000637643123	0.001663416843019656
34	0.0	0.0	0.0	0.33028065538623613	0.0018482409366885066
35	0.0	0.0	0.0	0.4062433578841338	0.0018482409366885066
36	0.0	0.0	0.0	0.5163985177107687	0.0018482409366885066
37	0.0	0.0	0.0	0.6816312574507213	0.0018482409366885066
38	0.0	0.0	0.0	0.8590623873728179	0.0018482409366885066
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAA	30	0.0057422454	29.333332	1
GTCCTAC	310	0.0	24.83871	1
GCATTCG	180	0.0	24.444443	22
GAGCGAA	145	0.0	22.75862	16
TGGACTA	60	2.8699386E-4	22.0	5
TGAACCG	50	0.0025786129	22.0	5
CTAAGAC	50	0.0025786129	22.0	3
CGGGGGC	200	0.0	22.0	17
AGCGAAA	140	7.2759576E-12	22.0	17
GTATAGA	90	4.0731902E-7	21.999998	1
GGCGAGA	205	0.0	21.463415	19
TCCTACA	360	0.0	21.388887	2
CCTACAG	355	0.0	21.070421	3
ATTCGTA	210	0.0	20.952381	24
GCGAGAA	200	0.0	20.9	20
CGCAATA	190	0.0	20.842106	36
AATCGCT	95	6.8518784E-7	20.842106	15
CGAATGC	180	0.0	20.777777	43
ATACGAA	180	0.0	20.777777	40
TAACGGC	75	5.4654767E-5	20.533333	36
>>END_MODULE