FastQCFastQC Report
Thu 2 Feb 2017
SRR4062679_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062679_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083834
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29550.14180592120101698No Hit
GCGCAAGACGGACCAGAGCGAAAGC28920.13878264775409174No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24940.1196832377243101No Hit
GTCTTGCGCCGGTCCAAGAATTTCA24850.11925134151760648No Hit
GAATAGGACCGCGGTTCTATTTTGT24620.11814760676714171No Hit
GTATCTGATCGTCTTCGAACCTCCG24340.1168039296796194No Hit
GGGTAGGCACACGCTGAGCCAGTCA24010.11522031025503951No Hit
GTGCATGGCCGTTCTTAGTTGGTGG22970.11022950964424229No Hit
GATTAAGAGGGACGGCCGGGGGCAT22760.10922175182860054No Hit
GAACTACGACGGTATCTGATCGTCT22680.10883784408930845No Hit
GAATAACGCCGCCGCATCGCCAGTC22410.10754215546919764No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC21850.10485480129415299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG1200.015.0426947
TCGCGCA456.760192E-414.7787889
GCGTTAT2750.013.4616781
TTAGACG500.001502062713.2973934
ACGGTAT3900.013.1547449
CAAGACG4600.013.008324
CGCATCG3300.012.66783613
AAGACGG4750.012.5996495
CGGTTCT4800.012.470812
CGTTATT2900.012.4413522
GTATCAA26850.012.3027511
TTATACC701.093242E-412.2118914
ATCGCCA3600.012.14205216
GGTTCTA5250.012.12492913
CCTATAC1459.822543E-1111.7885273
GGTATCA10650.011.7649921
CGCAAGA4950.011.7005592
CGAACGA2450.011.63568516
AGGCCCG4900.011.63344710
GATATAC4850.011.5472241
Produced by FastQC (version 0.11.5)