##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062679_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2083834
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227334327014532	32.0	32.0	32.0	32.0	32.0
2	30.900122082661095	32.0	32.0	32.0	32.0	32.0
3	30.8790095564234	32.0	32.0	32.0	32.0	32.0
4	30.95943198930433	32.0	32.0	32.0	32.0	32.0
5	30.776180348338688	32.0	32.0	32.0	32.0	32.0
6	34.4350015404298	36.0	36.0	36.0	32.0	36.0
7	34.32662102643493	36.0	36.0	36.0	32.0	36.0
8	34.3308243362955	36.0	36.0	36.0	32.0	36.0
9	34.47775878500879	36.0	36.0	36.0	32.0	36.0
10	34.15942296747246	36.0	36.0	36.0	32.0	36.0
11	34.44635273251132	36.0	36.0	36.0	32.0	36.0
12	34.24351651811037	36.0	36.0	36.0	32.0	36.0
13	34.359827126344996	36.0	36.0	36.0	32.0	36.0
14	34.26679572365169	36.0	36.0	36.0	32.0	36.0
15	34.206118625571904	36.0	36.0	36.0	32.0	36.0
16	34.21230769821396	36.0	36.0	36.0	32.0	36.0
17	34.13543209295942	36.0	36.0	36.0	32.0	36.0
18	34.14901666831427	36.0	36.0	36.0	32.0	36.0
19	34.144024907934124	36.0	36.0	36.0	32.0	36.0
20	34.13747304247843	36.0	36.0	36.0	32.0	36.0
21	34.11653519426211	36.0	36.0	36.0	32.0	36.0
22	34.08079338373402	36.0	36.0	36.0	32.0	36.0
23	34.04172693218366	36.0	36.0	36.0	32.0	36.0
24	34.00734223551396	36.0	36.0	36.0	32.0	36.0
25	33.57365269978319	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	9.0
4	89.0
5	184.0
6	379.0
7	68.0
8	304.0
9	383.0
10	247.0
11	72.0
12	149.0
13	113.0
14	257.0
15	357.0
16	532.0
17	829.0
18	1045.0
19	1504.0
20	2313.0
21	3504.0
22	5287.0
23	8000.0
24	11345.0
25	16228.0
26	22895.0
27	29542.0
28	40774.0
29	54172.0
30	70808.0
31	97441.0
32	141313.0
33	204655.0
34	466638.0
35	902396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.345463236430405	17.7400792641431	10.952283139428296	25.962174359998198
2	16.95173587399335	20.27749796881891	37.06606749423999	25.704698662947756
3	19.24328749872824	23.964258222298156	28.55085414383095	28.241600135142658
4	12.48728718178358	15.534523910657267	36.575767916614474	35.402420990944684
5	14.38510180293839	36.66830849468374	33.90902928598724	15.037560416390628
6	33.46173050517077	36.07021115197373	17.139654129418204	13.328404213437292
7	29.516973586398848	30.53363119305882	21.19859086482406	18.750804355718273
8	27.532385942548483	33.05731655010401	20.005725266918954	19.40457224042855
9	26.991642477292892	14.109798221474593	18.933855052052586	39.96470424917993
10	15.48090056822413	26.8396178143814	32.10877117628208	25.57071044111239
11	37.641037861855445	21.297702074993417	22.326694712485516	18.734565350665626
12	24.050615880362464	24.11519754589671	29.335789102420563	22.498397471320263
13	29.01128985296958	19.53450136402641	25.71403587499964	25.74017290800437
14	22.95096854045307	20.26031146277572	25.286218646637927	31.502501350133283
15	24.85050465937354	27.75965252031825	22.443013533970404	24.946829286337806
16	25.35774372357097	26.103497543619607	24.29251152284054	24.246247209968878
17	23.71324077001356	26.027644074848872	25.45065280875447	24.808462346383102
18	24.455550302135705	25.25927548933684	26.55344265571905	23.7317315528084
19	25.021274510133683	25.25667558330118	25.564249020603718	24.157800885961418
20	25.16574618816198	24.976781840588657	25.12188032257987	24.735591648669487
21	26.600687215211956	24.603140662892155	24.562105717359557	24.234066404536332
22	25.453864752989443	24.919343637675233	25.36802994702368	24.25876166231165
23	24.313864000595878	24.546784784943522	25.7156175139899	25.423733700470695
24	24.327817869564424	25.55762501573719	25.565409517658804	24.549147597039582
25	24.52174439506486	24.9499196840988	25.564337529556873	24.963998391279464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	154.0
1	154.0
2	497.0
3	840.0
4	840.0
5	840.0
6	1933.0
7	3026.0
8	3026.0
9	3026.0
10	3565.5
11	4105.0
12	4105.0
13	4105.0
14	5411.0
15	6717.0
16	6717.0
17	6717.0
18	10751.0
19	14785.0
20	14785.0
21	14785.0
22	23885.5
23	32986.0
24	32986.0
25	32986.0
26	49611.5
27	66237.0
28	66237.0
29	66237.0
30	83808.0
31	101379.0
32	101379.0
33	101379.0
34	122694.5
35	144010.0
36	144010.0
37	144010.0
38	161787.5
39	179565.0
40	179565.0
41	179565.0
42	202632.5
43	225700.0
44	225700.0
45	225700.0
46	247132.0
47	268564.0
48	268564.0
49	268564.0
50	270997.0
51	273430.0
52	273430.0
53	273430.0
54	251348.5
55	229267.0
56	229267.0
57	229267.0
58	208794.5
59	188322.0
60	188322.0
61	188322.0
62	165980.5
63	143639.0
64	143639.0
65	143639.0
66	119000.5
67	94362.0
68	94362.0
69	94362.0
70	71592.0
71	48822.0
72	48822.0
73	48822.0
74	38085.5
75	27349.0
76	27349.0
77	27349.0
78	22714.5
79	18080.0
80	18080.0
81	18080.0
82	12533.0
83	6986.0
84	6986.0
85	6986.0
86	5171.0
87	3356.0
88	3356.0
89	3356.0
90	2209.5
91	1063.0
92	1063.0
93	1063.0
94	638.0
95	213.0
96	213.0
97	213.0
98	545.0
99	877.0
100	877.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008062062525133959
2	0.003407181186217328
3	0.005278731415266283
4	0.014732459495334083
5	0.03791088925509421
6	0.04674076725881236
7	0.06823960065916959
8	0.0880588377001239
9	0.0958329694207888
10	0.11694789508185392
11	0.1189154222457259
12	0.13187230844683404
13	0.11977921465913312
14	0.12275449963864685
15	0.11186111753623369
16	0.11114129052506101
17	0.12093093787700941
18	0.1271694386405059
19	0.12980880434813905
20	0.11944329538725253
21	0.12865708113026278
22	0.1484283297038056
23	0.13767891300362697
24	0.13321598553435637
25	0.12846512726061673
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2083834.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.99062178707803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85419741326433	40.476881322495345
2	13.783858758436734	14.332627749527743
3	3.9686450609627855	6.189969731150142
4	1.6220703454764562	3.3732978337480577
5	0.7884388408813824	2.0495712789253075
6	0.44750396685065924	1.3959605693249837
7	0.2909504745229685	1.0588687255786176
8	0.2004696669805117	0.8338034108612216
9	0.15131344787445006	0.7080192215765352
>10	0.7103999717791223	6.736071944525077
>50	0.07623743898060449	2.7841171734324037
>100	0.08515827940159791	9.694690675710152
>500	0.014760060151799426	5.386656936092759
>1k	0.005996274436668517	4.979463427051628
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2955	0.14180592120101698	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2892	0.13878264775409174	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2494	0.1196832377243101	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2485	0.11925134151760648	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2462	0.11814760676714171	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2434	0.1168039296796194	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2401	0.11522031025503951	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2297	0.11022950964424229	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2276	0.10922175182860054	No Hit
GAACTACGACGGTATCTGATCGTCT	2268	0.10883784408930845	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2241	0.10754215546919764	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2185	0.10485480129415299	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.7988467411511665E-5
2	0.0	0.0	0.0	0.0	4.7988467411511665E-5
3	0.0	0.0	0.0	0.0	4.7988467411511665E-5
4	0.0	0.0	0.0	0.0	4.7988467411511665E-5
5	0.0	0.0	0.0	0.0	4.7988467411511665E-5
6	0.0	0.0	0.0	0.0	4.7988467411511665E-5
7	0.0	0.0	0.0	0.0	4.7988467411511665E-5
8	0.0	0.0	0.0	0.0	4.7988467411511665E-5
9	0.0	0.0	0.0	0.0	4.7988467411511665E-5
10	0.0	0.0	0.0	0.0	4.7988467411511665E-5
11	0.0	0.0	0.0	0.0	4.7988467411511665E-5
12	0.0	0.0	0.0	0.0	4.7988467411511665E-5
13	0.0	0.0	0.0	0.0	9.597693482302333E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	120	0.0	15.042694	7
TCGCGCA	45	6.760192E-4	14.778788	9
GCGTTAT	275	0.0	13.461678	1
TTAGACG	50	0.0015020627	13.297393	4
ACGGTAT	390	0.0	13.154744	9
CAAGACG	460	0.0	13.00832	4
CGCATCG	330	0.0	12.667836	13
AAGACGG	475	0.0	12.599649	5
CGGTTCT	480	0.0	12.4708	12
CGTTATT	290	0.0	12.441352	2
GTATCAA	2685	0.0	12.302751	1
TTATACC	70	1.093242E-4	12.211891	4
ATCGCCA	360	0.0	12.142052	16
GGTTCTA	525	0.0	12.124929	13
CCTATAC	145	9.822543E-11	11.788527	3
GGTATCA	1065	0.0	11.764992	1
CGCAAGA	495	0.0	11.700559	2
CGAACGA	245	0.0	11.635685	16
AGGCCCG	490	0.0	11.633447	10
GATATAC	485	0.0	11.547224	1
>>END_MODULE