FastQCFastQC Report
Thu 2 Feb 2017
SRR4062679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083834
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT27410.13153638917495347No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT25650.12309041891052742No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24830.11915536458278347No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24630.11819559523455322No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA24400.11709186048408846No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23860.11450048324386684No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC23070.11070939431835741No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC21660.10394302041333427No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG21600.10365508960886521No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG21390.10264733179322345No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT21390.10264733179322345No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT21250.10197549324946228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29000.022.686471
TAGGACG4600.021.0433864
CGTCTTA2250.020.53324515
AGGACGT4600.019.130355
GATATAC5800.018.5892471
GGTATCA14000.018.545891
CGAGCGT600.00741404418.33325228
TCGGTAT600.00741404418.33325212
AACGCAG39300.016.5699026
GGACGTG5350.016.4485266
TTAGGAC4950.016.4443723
GACGTGA3100.016.322517
ATACACT7700.016.2856434
ACTGTTC8700.016.1838388
ATACCGT6300.016.0634216
CGGGATT1958.931238E-1015.79480444
ACCGTCG6550.015.7861918
CGTCGTA6150.015.73976810
CCGTCGT6100.015.508139
TCAACGC42250.015.412954
Produced by FastQC (version 0.11.5)