##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062679_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2083834
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34382201269391	32.0	32.0	32.0	32.0	32.0
2	31.432559887207905	32.0	32.0	32.0	32.0	32.0
3	31.514429172381295	32.0	32.0	32.0	32.0	32.0
4	31.614489925781037	32.0	32.0	32.0	32.0	32.0
5	31.554367574384525	32.0	32.0	32.0	32.0	32.0
6	35.16398619083862	36.0	36.0	36.0	36.0	36.0
7	35.17402345868241	36.0	36.0	36.0	36.0	36.0
8	35.123231984889394	36.0	36.0	36.0	36.0	36.0
9	35.24432272436288	36.0	36.0	36.0	36.0	36.0
10	35.12427765359429	36.0	36.0	36.0	36.0	36.0
11	35.25157282201941	36.0	36.0	36.0	36.0	36.0
12	35.162414088646216	36.0	36.0	36.0	36.0	36.0
13	35.2164246288332	36.0	36.0	36.0	36.0	36.0
14	35.18052445636265	36.0	36.0	36.0	36.0	36.0
15	35.13446512534108	36.0	36.0	36.0	36.0	36.0
16	35.15369410423287	36.0	36.0	36.0	36.0	36.0
17	35.12435779433486	36.0	36.0	36.0	36.0	36.0
18	35.14094164890294	36.0	36.0	36.0	36.0	36.0
19	35.126723625778254	36.0	36.0	36.0	36.0	36.0
20	35.119442335617904	36.0	36.0	36.0	36.0	36.0
21	35.120760098933026	36.0	36.0	36.0	36.0	36.0
22	35.10170483829326	36.0	36.0	36.0	36.0	36.0
23	35.07101525361425	36.0	36.0	36.0	36.0	36.0
24	35.04367190476785	36.0	36.0	36.0	36.0	36.0
25	35.01909413129837	36.0	36.0	36.0	36.0	36.0
26	34.984431101517686	36.0	36.0	36.0	36.0	36.0
27	34.97329297823147	36.0	36.0	36.0	36.0	36.0
28	34.959717040800754	36.0	36.0	36.0	32.0	36.0
29	34.93518293683662	36.0	36.0	36.0	32.0	36.0
30	34.92504777251931	36.0	36.0	36.0	32.0	36.0
31	34.92742560107955	36.0	36.0	36.0	32.0	36.0
32	34.898354187521655	36.0	36.0	36.0	32.0	36.0
33	34.88332323975902	36.0	36.0	36.0	32.0	36.0
34	34.875857673883814	36.0	36.0	36.0	32.0	36.0
35	34.8542801393969	36.0	36.0	36.0	32.0	36.0
36	34.829447547165465	36.0	36.0	36.0	32.0	36.0
37	34.82956847810334	36.0	36.0	36.0	32.0	36.0
38	34.800812828661016	36.0	36.0	36.0	32.0	36.0
39	34.80049178581403	36.0	36.0	36.0	32.0	36.0
40	34.798830425072246	36.0	36.0	36.0	32.0	36.0
41	34.76550531376299	36.0	36.0	36.0	32.0	36.0
42	34.74692369929659	36.0	36.0	36.0	32.0	36.0
43	34.7439033051577	36.0	36.0	36.0	32.0	36.0
44	34.71327274629361	36.0	36.0	36.0	32.0	36.0
45	34.69105792495947	36.0	36.0	36.0	32.0	36.0
46	34.689027532903296	36.0	36.0	36.0	32.0	36.0
47	34.6703696167737	36.0	36.0	36.0	32.0	36.0
48	34.627308605196	36.0	36.0	36.0	32.0	36.0
49	34.616275096768746	36.0	36.0	36.0	32.0	36.0
50	34.12214312656383	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	12.0
21	39.0
22	106.0
23	357.0
24	900.0
25	2259.0
26	4841.0
27	9303.0
28	16452.0
29	26676.0
30	40623.0
31	60570.0
32	92826.0
33	159660.0
34	364299.0
35	1304909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9243463870341	18.319209636111612	11.425123926323833	26.331320050530454
2	16.3761776968971	20.790093524682685	36.71808412614314	26.11564465227707
3	18.676232409151442	24.28582054635106	28.875169460007438	28.162777584490062
4	12.167301475023647	16.088060884016027	36.733401125139224	35.011236515821096
5	14.0968590093352	36.91596207565577	33.82545530280018	15.161723612208847
6	33.71015676475401	36.38063309599712	16.75257447489542	13.15663566435345
7	29.58112746133086	30.644025046164952	21.15679191028835	18.61805558221584
8	27.607237428701136	33.26263032468037	19.776527304958073	19.35360494166042
9	27.25159489671442	13.884647241574905	18.772464601307014	40.09129326040366
10	15.596395874143527	26.96102472653772	32.03393360507603	25.40864579424273
11	37.8478803973829	21.292147071215844	22.235696317460988	18.624276213940266
12	24.099664368658924	24.13800715412072	29.184378410180468	22.57795006703989
13	29.20146230457896	19.544215134218945	25.650363704594515	25.603958856607576
14	23.078805701413838	20.252908820952147	25.25441085998213	31.41387461765189
15	24.95481885793206	27.87218175728009	22.229361839762667	24.94363754502518
16	25.5606732590024	26.08561910401692	24.136807442435433	24.216900194545246
17	23.876853914467276	26.16940696811742	25.241118054509144	24.712621062906162
18	24.65215559396766	25.278741012959767	26.450715364083706	23.618388028988875
19	25.25037982871956	25.28646715621302	25.395592931106798	24.067560083960622
20	25.403511028229698	24.908845906151832	24.962640978120138	24.725002087498332
21	26.79589641017471	24.623794409727452	24.405926767679194	24.174382412418645
22	25.613220630818002	24.970127179036332	25.169135353391876	24.24751683675379
23	24.513085015409096	24.50463904514467	25.489842281103	25.492433658343227
24	24.567311983584105	25.56763158677707	25.31948322179214	24.54557320784669
25	24.71602824409238	24.934231805412523	25.425201815499697	24.924538134995398
26	24.039150911253007	26.09948777109885	26.025057658143595	23.836303659504548
27	25.076181692015776	25.07162278761168	25.254026952242835	24.598168568129708
28	24.210853647651394	25.3293208576115	25.759873387227582	24.699952107509525
29	24.1942016494596	25.053435158462715	25.69350533679746	25.05885785528022
30	24.003207549161782	25.709197565641023	25.92351406110084	24.364080824096355
31	24.739110696917315	25.294001345596627	24.880484721911632	25.08640323557443
32	24.508094214798298	25.34995589859845	24.871462890038266	25.270486996564983
33	24.192330099230553	24.971566833058677	25.578285026542417	25.257818041168346
34	24.948964264907858	25.20387900379781	25.687986663045137	24.159170068249196
35	25.46795953996336	24.94531714138458	25.593833289983753	23.99289002866831
36	23.96937567963667	25.702575157138234	25.258729822049165	25.069319341175927
37	25.129400902375142	25.456346330849772	25.06082538244409	24.353427384331
38	24.27309468988413	25.050123954211323	25.5669597482333	25.109821607671247
39	25.008901860704835	24.87539794436601	25.015764211544685	25.099935983384476
40	25.38589926068967	25.130408660190785	25.508797725730552	23.974894353388994
41	24.039390853590064	25.532024143957727	26.23217588349168	24.19640911896053
42	25.411717056157062	25.74336535443802	25.409605563590958	23.435312025813957
43	24.46879165998827	24.665352422505823	25.809301508661438	25.056554408844466
44	23.992842040200898	25.410277402134717	25.357106180242766	25.239774377421618
45	24.232352481051752	25.53475948660018	25.573342214399037	24.65954581794903
46	24.102505531883857	25.076721746362107	25.524023324370663	25.296749397383373
47	24.377097396960313	24.884767637329862	25.89012651205735	24.848008453652476
48	25.094993171241086	25.420115037954076	24.96811166340505	24.516780127399784
49	24.04308596558075	25.678916842704364	25.422850380596536	24.855146811118352
50	24.070679334342373	25.76908717297059	25.283923767440207	24.876309725246827
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	10.0
2	16.0
3	48.0
4	80.0
5	116.5
6	153.0
7	154.5
8	156.0
9	218.0
10	280.0
11	421.5
12	563.0
13	983.5
14	1404.0
15	1998.5
16	2593.0
17	3174.0
18	3755.0
19	4340.5
20	4926.0
21	6168.0
22	7410.0
23	8504.5
24	9599.0
25	12469.0
26	15339.0
27	20209.5
28	25080.0
29	28830.5
30	32581.0
31	35735.0
32	38889.0
33	44556.5
34	50224.0
35	58247.5
36	66271.0
37	78001.0
38	89731.0
39	95272.5
40	100814.0
41	108060.5
42	115307.0
43	117127.5
44	118948.0
45	128688.5
46	138429.0
47	146127.0
48	153825.0
49	158559.0
50	163293.0
51	154935.5
52	146578.0
53	142178.5
54	137779.0
55	138784.5
56	139790.0
57	135506.5
58	131223.0
59	118959.0
60	106695.0
61	93643.0
62	80591.0
63	70117.0
64	59643.0
65	51166.0
66	42689.0
67	37383.0
68	32077.0
69	29178.0
70	26279.0
71	20026.5
72	13774.0
73	12434.5
74	11095.0
75	7883.0
76	4671.0
77	4295.5
78	3920.0
79	3398.5
80	2877.0
81	2299.5
82	1722.0
83	1488.5
84	1255.0
85	1084.5
86	914.0
87	681.0
88	448.0
89	270.5
90	93.0
91	60.0
92	27.0
93	18.5
94	10.0
95	7.5
96	5.0
97	5.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016220101985090944
2	0.005086777545620236
3	4.31896206703605E-4
4	1.43965402234535E-4
5	4.798846741151167E-5
6	5.278731415266284E-4
7	9.597693482302334E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.399423370575583E-4
47	4.798846741151167E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2083834.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.46271940777992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.10826373192585	42.53988451060751
2	13.834728482613166	15.069538708627636
3	3.947884588576879	6.450375918058839
4	1.5573707437709428	3.3927458332752956
5	0.7268430777425192	1.9792925298288997
6	0.42180977972479256	1.37837446059653
7	0.2690084439775471	1.0255651981870844
8	0.18537381489257648	0.8076769652835303
9	0.12614626278740265	0.6183241663077343
>10	0.6667312798973644	6.545034055968857
>50	0.06425562683506267	2.4720460220023526
>100	0.07402892749663546	8.753250804257682
>500	0.012526854501637255	4.6588311518719925
>1k	0.00502838525769946	4.309059675126005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2741	0.13153638917495347	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2565	0.12309041891052742	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2483	0.11915536458278347	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2463	0.11819559523455322	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2440	0.11709186048408846	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2386	0.11450048324386684	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2307	0.11070939431835741	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2166	0.10394302041333427	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2160	0.10365508960886521	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2139	0.10264733179322345	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	2139	0.10264733179322345	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2125	0.10197549324946228	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.7988467411511665E-5	0.0
5	0.0	0.0	0.0	4.7988467411511665E-5	0.0
6	0.0	0.0	0.0	4.7988467411511665E-5	4.7988467411511665E-5
7	0.0	0.0	0.0	4.7988467411511665E-5	4.7988467411511665E-5
8	0.0	0.0	0.0	4.7988467411511665E-5	4.7988467411511665E-5
9	0.0	0.0	0.0	4.7988467411511665E-5	4.7988467411511665E-5
10	0.0	0.0	0.0	9.597693482302333E-5	4.7988467411511665E-5
11	0.0	0.0	0.0	9.597693482302333E-5	4.7988467411511665E-5
12	0.0	0.0	0.0	9.597693482302333E-5	4.7988467411511665E-5
13	0.0	0.0	0.0	9.597693482302333E-5	4.7988467411511665E-5
14	0.0	0.0	0.0	9.597693482302333E-5	9.597693482302333E-5
15	0.0	0.0	0.0	9.597693482302333E-5	9.597693482302333E-5
16	0.0	0.0	0.0	9.597693482302333E-5	9.597693482302333E-5
17	0.0	0.0	0.0	9.597693482302333E-5	9.597693482302333E-5
18	0.0	0.0	0.0	9.597693482302333E-5	9.597693482302333E-5
19	0.0	0.0	0.0	9.597693482302333E-5	9.597693482302333E-5
20	0.0	0.0	0.0	1.9195386964604666E-4	9.597693482302333E-5
21	0.0	0.0	0.0	1.9195386964604666E-4	9.597693482302333E-5
22	0.0	4.7988467411511665E-5	0.0	1.9195386964604666E-4	9.597693482302333E-5
23	0.0	4.7988467411511665E-5	0.0	5.7586160893814E-4	1.43965402234535E-4
24	0.0	4.7988467411511665E-5	0.0	7.19827011172675E-4	1.43965402234535E-4
25	0.0	4.7988467411511665E-5	0.0	7.19827011172675E-4	1.43965402234535E-4
26	0.0	4.7988467411511665E-5	0.0	0.001007757815641745	1.43965402234535E-4
27	0.0	4.7988467411511665E-5	0.0	0.0011997116852877916	1.43965402234535E-4
28	0.0	4.7988467411511665E-5	0.0	0.002543388772810118	1.43965402234535E-4
29	0.0	4.7988467411511665E-5	0.0	0.0057586160893814	1.43965402234535E-4
30	0.0	4.7988467411511665E-5	0.0	0.014348551756041989	1.43965402234535E-4
31	0.0	4.7988467411511665E-5	0.0	0.03316003098135456	1.43965402234535E-4
32	0.0	4.7988467411511665E-5	0.0	0.059073803383570864	1.43965402234535E-4
33	0.0	4.7988467411511665E-5	0.0	0.086379241340721	1.43965402234535E-4
34	4.7988467411511665E-5	4.7988467411511665E-5	0.0	0.12083496094218638	1.9195386964604666E-4
35	4.7988467411511665E-5	4.7988467411511665E-5	0.0	0.16095331969821014	1.9195386964604666E-4
36	4.7988467411511665E-5	4.7988467411511665E-5	0.0	0.22655355464974658	1.9195386964604666E-4
37	4.7988467411511665E-5	4.7988467411511665E-5	0.0	0.3206109507763094	1.9195386964604666E-4
38	4.7988467411511665E-5	4.7988467411511665E-5	0.0	0.462752791249207	1.9195386964604666E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2900	0.0	22.68647	1
TAGGACG	460	0.0	21.043386	4
CGTCTTA	225	0.0	20.533245	15
AGGACGT	460	0.0	19.13035	5
GATATAC	580	0.0	18.589247	1
GGTATCA	1400	0.0	18.54589	1
CGAGCGT	60	0.007414044	18.333252	28
TCGGTAT	60	0.007414044	18.333252	12
AACGCAG	3930	0.0	16.569902	6
GGACGTG	535	0.0	16.448526	6
TTAGGAC	495	0.0	16.444372	3
GACGTGA	310	0.0	16.32251	7
ATACACT	770	0.0	16.285643	4
ACTGTTC	870	0.0	16.183838	8
ATACCGT	630	0.0	16.063421	6
CGGGATT	195	8.931238E-10	15.794804	44
ACCGTCG	655	0.0	15.786191	8
CGTCGTA	615	0.0	15.739768	10
CCGTCGT	610	0.0	15.50813	9
TCAACGC	4225	0.0	15.41295	4
>>END_MODULE