##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062678_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174
Sequences flagged as poor quality	0
Sequence length	25
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.358603066439525	32.0	32.0	32.0	32.0	32.0
2	30.948892674616694	32.0	32.0	32.0	32.0	32.0
3	31.094548551959114	32.0	32.0	32.0	32.0	32.0
4	31.08603066439523	32.0	32.0	32.0	32.0	32.0
5	30.637989778534923	32.0	32.0	32.0	32.0	32.0
6	34.695911413969334	36.0	36.0	36.0	32.0	36.0
7	34.30068143100511	36.0	36.0	36.0	32.0	36.0
8	34.369676320272575	36.0	36.0	36.0	32.0	36.0
9	34.59114139693356	36.0	36.0	36.0	32.0	36.0
10	34.48211243611584	36.0	36.0	36.0	32.0	36.0
11	34.724020442930154	36.0	36.0	36.0	32.0	36.0
12	34.45485519591141	36.0	36.0	36.0	32.0	36.0
13	34.33304940374787	36.0	36.0	36.0	32.0	36.0
14	34.36541737649063	36.0	36.0	36.0	32.0	36.0
15	34.35008517887564	36.0	36.0	36.0	32.0	36.0
16	34.441226575809196	36.0	36.0	36.0	32.0	36.0
17	34.228279386712096	36.0	36.0	36.0	32.0	36.0
18	34.19080068143101	36.0	36.0	36.0	32.0	36.0
19	34.11499148211244	36.0	36.0	36.0	32.0	36.0
20	34.28449744463373	36.0	36.0	36.0	32.0	36.0
21	34.21039182282794	36.0	36.0	36.0	32.0	36.0
22	34.14991482112436	36.0	36.0	36.0	32.0	36.0
23	33.9045996592845	36.0	36.0	36.0	32.0	36.0
24	34.12350936967632	36.0	36.0	36.0	32.0	36.0
25	33.85604770017036	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	0.0
17	1.0
18	0.0
19	1.0
20	2.0
21	4.0
22	2.0
23	4.0
24	5.0
25	7.0
26	5.0
27	17.0
28	30.0
29	26.0
30	34.0
31	51.0
32	84.0
33	90.0
34	246.0
35	563.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.826235093696766	17.717206132879046	9.114139693356048	27.342419080068144
2	24.190800681431003	17.632027257240203	30.40885860306644	27.76831345826235
3	26.575809199318567	18.05792163543441	25.979557069846678	29.38671209540034
4	21.312872975277067	14.663256606990622	31.9693094629156	32.05456095481671
5	23.35890878090367	30.861040068201195	29.838022165387894	15.942028985507244
6	41.17647058823529	29.326513213981244	14.23699914748508	15.26001705029838
7	35.80562659846547	27.365728900255753	17.647058823529413	19.18158567774936
8	32.93515358361775	28.071672354948806	19.283276450511945	19.7098976109215
9	31.569965870307165	14.334470989761092	19.453924914675767	34.641638225255974
10	26.109215017064848	21.928327645051194	26.535836177474405	25.426621160409557
11	41.894197952218434	18.941979522184297	19.965870307167236	19.197952218430032
12	28.86421861656704	20.40990606319385	25.78992314261315	24.93595217762596
13	34.8976109215017	18.515358361774744	21.160409556313994	25.426621160409557
14	28.693424423569596	16.737830913748933	22.032450896669513	32.53629376601195
15	31.25533731853117	24.25277540563621	21.77625960717336	22.715627668659266
16	31.8531169940222	22.801024765157983	22.28864218616567	23.05721605465414
17	31.48464163822526	20.989761092150168	23.378839590443686	24.14675767918089
18	29.63279248505551	22.544833475661825	23.740392826643895	24.08198121263877
19	32.9059829059829	24.615384615384617	22.47863247863248	20.0
20	33.39026473099915	21.861656703672075	20.922288642186164	23.82578992314261
21	31.169940222032448	24.765157984628523	22.544833475661825	21.5200683176772
22	33.81725021349274	21.43467122117848	20.40990606319385	24.338172502134928
23	32.19470538001708	21.77625960717336	19.89752348420154	26.131511528608026
24	31.93851409052092	20.40990606319385	22.28864218616567	25.362937660119556
25	31.08454312553373	24.850555081127244	21.690862510674638	22.37403928266439
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	1.0
7	1.0
8	1.0
9	1.0
10	2.5
11	4.0
12	4.0
13	4.0
14	3.0
15	2.0
16	2.0
17	2.0
18	9.5
19	17.0
20	17.0
21	17.0
22	19.0
23	21.0
24	21.0
25	21.0
26	25.5
27	30.0
28	30.0
29	30.0
30	44.0
31	58.0
32	58.0
33	58.0
34	62.5
35	67.0
36	67.0
37	67.0
38	70.0
39	73.0
40	73.0
41	73.0
42	84.5
43	96.0
44	96.0
45	96.0
46	109.5
47	123.0
48	123.0
49	123.0
50	114.5
51	106.0
52	106.0
53	106.0
54	103.5
55	101.0
56	101.0
57	101.0
58	100.5
59	100.0
60	100.0
61	100.0
62	88.5
63	77.0
64	77.0
65	77.0
66	74.0
67	71.0
68	71.0
69	71.0
70	60.5
71	50.0
72	50.0
73	50.0
74	45.0
75	40.0
76	40.0
77	40.0
78	30.0
79	20.0
80	20.0
81	20.0
82	14.5
83	9.0
84	9.0
85	9.0
86	6.5
87	4.0
88	4.0
89	4.0
90	3.0
91	2.0
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	2.0
98	50.5
99	99.0
100	99.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.08517887563884156
5	0.08517887563884156
6	0.08517887563884156
7	0.08517887563884156
8	0.17035775127768313
9	0.17035775127768313
10	0.17035775127768313
11	0.17035775127768313
12	0.2555366269165247
13	0.17035775127768313
14	0.2555366269165247
15	0.2555366269165247
16	0.2555366269165247
17	0.17035775127768313
18	0.2555366269165247
19	0.34071550255536626
20	0.2555366269165247
21	0.2555366269165247
22	0.2555366269165247
23	0.2555366269165247
24	0.2555366269165247
25	0.2555366269165247
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1174.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.73424190800682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.33980582524272	83.64565587734242
2	4.368932038834951	7.666098807495741
3	0.1941747572815534	0.5110732538330494
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0970873786407767	8.17717206132879
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	96	8.17717206132879	No Hit
GGTCACGTGGGCCGCGGCACCGCAT	3	0.2555366269165247	No Hit
CCTTCCAAGCTGCCACTTCCTTCAG	3	0.2555366269165247	No Hit
ATACAGTGGAATATTATTTATCCTG	2	0.17035775127768313	No Hit
TATTTGTCAAGGCACCTTTTTTTTT	2	0.17035775127768313	No Hit
CAACAAAATTTGCTTAAAAACAAAA	2	0.17035775127768313	No Hit
GGTCCCAGAGGTGGTGTAGCAAGTG	2	0.17035775127768313	No Hit
GCTATAATATGCAGAGGCAGAAGTA	2	0.17035775127768313	No Hit
GATTTCCTCATCTGGGGCTAAATAT	2	0.17035775127768313	No Hit
TTCTTCACCACATCGCCGGCCACCA	2	0.17035775127768313	No Hit
CCCCAGGGCAGCTCCAGGTGGAGGG	2	0.17035775127768313	No Hit
GCCTCATTGTTCGCGGGCGCTCCAA	2	0.17035775127768313	No Hit
ATTTGGGGCAGAAGAGGCAGTCTTA	2	0.17035775127768313	No Hit
ACGCACATGTGTTCACACAAACACA	2	0.17035775127768313	No Hit
CAGTTTTTCCTGAGAAAGTGGCCAC	2	0.17035775127768313	No Hit
TTCAGAGACTGTGTTTCCTCCTAGA	2	0.17035775127768313	No Hit
GCCTGCTGGCAGCGCTCTTGACCAA	2	0.17035775127768313	No Hit
CGTCCGTTCCACCCAGTTGGCAGGC	2	0.17035775127768313	No Hit
TCAAAATATTGCTTCAAATGAAACA	2	0.17035775127768313	No Hit
GGCAGAACTTACACAAATGCTTGTG	2	0.17035775127768313	No Hit
GTCCCTGCTTATTGCGACCCACTTC	2	0.17035775127768313	No Hit
GTCCGTACTACCCAAAGCAATCTAC	2	0.17035775127768313	No Hit
CTCTCGGGACTCGAGAATTCCAATC	2	0.17035775127768313	No Hit
GGCTTCATTGCATTAAGGGGCATTA	2	0.17035775127768313	No Hit
CTCATACAAAGTGAAGTAAGTCAGA	2	0.17035775127768313	No Hit
CTTTATGCGGGATCTGCCGACGGAG	2	0.17035775127768313	No Hit
CAAGTGGATCGTGATTGAGCTAGCC	2	0.17035775127768313	No Hit
CCACCACTCTGCTGATTTCACCCCT	2	0.17035775127768313	No Hit
GTGTTCATTTAGTCCAATCAGGATA	2	0.17035775127768313	No Hit
CAGCTAATTTTTGTATTTTTAGTAG	2	0.17035775127768313	No Hit
GGCCTGCTGCGCACCGCCGTGCGCG	2	0.17035775127768313	No Hit
TCTTTACACATTCACAGATGGCCAT	2	0.17035775127768313	No Hit
GCTGAGCCTCAATGGCCCACGGCAC	2	0.17035775127768313	No Hit
ATCTGGAGCTGAGTCTTTAAGACGT	2	0.17035775127768313	No Hit
GGGCAGTGGCTCAGACTGCGGAGGC	2	0.17035775127768313	No Hit
CAATAGTGGAGATAAAATGGAACCA	2	0.17035775127768313	No Hit
TCTTTAATACATGGCAGATTCTTTG	2	0.17035775127768313	No Hit
CCCCCAAACCGAGCGACCACAGATG	2	0.17035775127768313	No Hit
ATGTCAGGAAGCGAAGGAGTTCAGA	2	0.17035775127768313	No Hit
GCTCGCGCTCGTCATCCCGCAGATG	2	0.17035775127768313	No Hit
TATCTACCCAGAACAGAACTAAATA	2	0.17035775127768313	No Hit
GGATGGGTCTGCCTTTCTTGTGCCC	2	0.17035775127768313	No Hit
GCTCGCCACGAGCGGATTGCTCGAT	2	0.17035775127768313	No Hit
GTAAAAGGTTTTGGTGGGCCGGGTG	2	0.17035775127768313	No Hit
GCCCCTCAACAGAGGAATGGATACA	2	0.17035775127768313	No Hit
CATTAGCGCAGGGAAAGGAGATTCA	2	0.17035775127768313	No Hit
ATATTCACATACACACAAAATACAC	2	0.17035775127768313	No Hit
CATTTACATTTAAGGTTAATATTGT	2	0.17035775127768313	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE