FastQCFastQC Report
Thu 2 Feb 2017
SRR4062678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT292.4701873935264054No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT80.6814310051107325No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTT60.5110732538330494No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT50.42589437819420783No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGG50.42589437819420783No Hit
TCCCTGGACCACACATTCTCCCTTCAGGAAAGTTTTTTGCAGAAGCTGTG30.2555366269165247No Hit
GCTTCATCCGCCAGTGCGGGGCGGTGAAGACGTCCACTTCATCGGGGAAG30.2555366269165247No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC30.2555366269165247No Hit
GTATTACTAAGAATGGCAGAAAAGTTTTACATTCAGCATCAACCTTCACA20.17035775127768313No Hit
CCATTGGTGAAGATGACCAGGAGTTTGAGAAGGAGAATGAAGTCTTCCGA20.17035775127768313No Hit
CTGCAAAAACATGCCAAATTGTAAAGACCATCGAGGCTAGGAAGAAACTG20.17035775127768313No Hit
AGTCTGCAGCGCCTCGAGCGCCGTGTTTTCCTCCGTCGGCAGATCCCGCA20.17035775127768313No Hit
CTTCCAGAAAGTGTGGCCCAGACACTTGCTACACCACCTCTGGGACCCTG20.17035775127768313No Hit
GATTAAGGATAATTTATCAAAAAATGAAGATGTGGGTGGGGGTAGTAATT20.17035775127768313No Hit
CCCGTTGGCCGCAAGCGACCCCTGGGTCGATGCCGCGATCGACTGGGACG20.17035775127768313No Hit
TTGCCTCTACTGTGAGCAAGATGAACATGAACCTTTGTGCATAAAACCTG20.17035775127768313No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTGT20.17035775127768313RNA PCR Primer, Index 7 (95% over 21bp)
AGGGACTCCTGCATGTTGACGCCGGCGGCGATGAGTCGCAGGAACATCGG20.17035775127768313No Hit
GCCCATCGTCGCCTGGGACGGCGGTTTGACGTCGGCCACGGCTTAAGCCT20.17035775127768313No Hit
CCTTCGAGCAGGTTGGAGCGCCCGCGAACAATGAGGCCTGTCTCTTATAC20.17035775127768313No Hit
GTACAGATGCAAGGGAGGGAAGAGGGCCTGGCCAGAAGGTAGGGCTAGCA20.17035775127768313No Hit
GTGGAAAGGTTCATTTGTGCTGTATATTAGATTCCAGATATAAGTGATAT20.17035775127768313No Hit
CTCTCTTTCTCTCTCTCTCACACATGCATATATGTGTGAGAGAGAGAGAA20.17035775127768313No Hit
CGCATGCGGCTAGGGGATGGTGGAGTCTCTTCTAACTTGGGTCTGACGTT20.17035775127768313No Hit
ACATTGAACTGTGCCTCGCCGTCCGACTCCGAAATGGCTAGCTCAATCAC20.17035775127768313No Hit
ATTCTCAAAAGCTCATTTGGTAAAGATTGAGGAGCACCGAATGAGCCTCA20.17035775127768313No Hit
GCTTTGCCGTCCAAGTCAGGATGTCTATTGTTGTCCTTGTTCAGCTAATG20.17035775127768313No Hit
CTACAAAGGTAAGTCTCATCCAAGTACAAAAGGGCACAAGAAAGGCAGAC20.17035775127768313No Hit
ACTATAATGCCCCTTAATGCAATGAAGCCCTGTCTCTTATACACATCTCC20.17035775127768313No Hit
ATTATAAATCGTTGCATATTTCCTATCCAAATGAACAAGATGCCGCTTAT20.17035775127768313No Hit
GAAGAAAAGTTAGTGGTTCTTTCCTTTTTCATCTAAAACTGTTTCATTTG20.17035775127768313No Hit
ACTCCATTGTGTAGATGTACCACATTTTCTGTATCCATTCCTCTGTTGAG20.17035775127768313No Hit
ATCCTGTTGCTGATCCATGCTGCCGTTGAAGGAAGATATTTAGTTCTGTT20.17035775127768313No Hit
TGTCAGAACTTCTGTGCTTTTCAGGATAAATAATATTCCACTGTATCTGT20.17035775127768313No Hit
CTGCCCACTCTGAGTATGACATTGTTCTGCTTTCTGGAAGGATTTACCTT20.17035775127768313No Hit
CGTCAACACTTGGCCTGCTGCGCCGACAACGGCACGTCGAATACCTTGTC20.17035775127768313No Hit
CACCTGTTGTGACCTTCATCTTTGTTACTGACCATGTCATATCACAAGCA20.17035775127768313No Hit
TTTTGATAGAGATTGAAATGAATCTGTAGATTGCTTTGGGTAGTACGGAC20.17035775127768313No Hit
ACTTGAGCTGGCTTGAAGGCTAGTGCTTCTGCCTGCCTGCCCTTCCCTGG20.17035775127768313No Hit
ACATAGGCTCAAAATAAAGGGATGGAGGAATATTTTCCAAGTAAAAGGAA20.17035775127768313No Hit
TCCTTCGACAGCAGCCGCGTGCGCATCGCCGAAGTGAAGCGCGGCGACCT20.17035775127768313No Hit
CACTATATTGGTGAATTTATCCTGATTGGACTAAATGAACACCTGTCTCT20.17035775127768313No Hit
AAGTCCTATTCCCTTAAAATGGAAGACCCAAAATTCTGTTTGGGTCAATC20.17035775127768313No Hit
GACATGTCCCGCAATCCTTTTATGCAAAACATGCCTGAAATCCAGGCCGC20.17035775127768313No Hit
GCTCTCGAAGGGCTCCTTTGAGGGCGTCGGCGAGGATGCCCGCCGCGTTC20.17035775127768313No Hit
AGACCCAGGAGATGGTCTTCGACGCGCACACCCGCGCCTTTGCACTGTTC20.17035775127768313No Hit
TAGCTATACTGTTCATCAATTGTCCATCTTCAGTGTCCATCAATGAGGAA20.17035775127768313No Hit
AGGCAGGGCTATGGAATTACTCTCCTGGAAAACCTTTCCAGACAGGGGTG20.17035775127768313No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC20.17035775127768313RNA PCR Primer, Index 41 (95% over 22bp)
GCATGAGCCACCGCACCCGGCCCACCAAAACCTTTTACCTGTCTCTTATA20.17035775127768313No Hit
GGAAGGCGATGGTCAACGGCCCGTCCGGCAAGGTGGCAAGATGCTGCGGA20.17035775127768313No Hit
AGCATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGCTGTC20.17035775127768313No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTGTT20.17035775127768313No Hit
CCCTGATATTCATGGATATACAATCTGAAAAGTTTGGCCATTCATAAGAC20.17035775127768313No Hit
GTCATTGCCAGAGTGGAAGCTAGCACCCTGATCTACTTCTGCCTCTGCAT20.17035775127768313No Hit
TGCCAAGATCGCCCTGAAGAACATTGCCACAGTAGGCGACGAACTGCGCC20.17035775127768313No Hit
ACATAGTATTCCCCCCTCCACCTGGAGCTGCCCTGGGGCTGTCTCTTATA20.17035775127768313No Hit
GTATAACATTGGAAATGTAAATGAGCTAAATACCTAATAAAAAATGGAAA20.17035775127768313No Hit
GAGATGAGACCAGAACTGTGGCTCGCGGACTACCGGCTGGCCTGCCAACT20.17035775127768313No Hit
GATATGAACATGCAGATGCGGGACTTCCTGGTGTGAATGCTGGCCGGAAT20.17035775127768313No Hit
GCTTAGCATAATGTCCTCCAGATCCATCTATGTTGAAAATGGCCCAGTTT20.17035775127768313No Hit
GGCCCATGGCGATACCGGCAACCGGACGTGCGAGCGGAACGCCTGCGTCC20.17035775127768313No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)