##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062678_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.471039182282794	32.0	32.0	32.0	32.0	32.0
2	31.244463373083477	32.0	32.0	32.0	32.0	32.0
3	31.658432708688245	32.0	32.0	32.0	32.0	32.0
4	31.722316865417376	32.0	32.0	32.0	32.0	32.0
5	31.581771720613286	32.0	32.0	32.0	32.0	32.0
6	35.10221465076661	36.0	36.0	36.0	36.0	36.0
7	35.20442930153322	36.0	36.0	36.0	36.0	36.0
8	35.130323679727425	36.0	36.0	36.0	36.0	36.0
9	35.28109028960818	36.0	36.0	36.0	36.0	36.0
10	35.25468483816014	36.0	36.0	36.0	36.0	36.0
11	35.1252129471891	36.0	36.0	36.0	36.0	36.0
12	35.0221465076661	36.0	36.0	36.0	36.0	36.0
13	35.07410562180579	36.0	36.0	36.0	36.0	36.0
14	35.176320272572404	36.0	36.0	36.0	36.0	36.0
15	35.170357751277685	36.0	36.0	36.0	36.0	36.0
16	35.246166950596255	36.0	36.0	36.0	36.0	36.0
17	35.16269165247019	36.0	36.0	36.0	36.0	36.0
18	35.100511073253834	36.0	36.0	36.0	36.0	36.0
19	35.05195911413969	36.0	36.0	36.0	36.0	36.0
20	35.0221465076661	36.0	36.0	36.0	36.0	36.0
21	34.84838160136286	36.0	36.0	36.0	32.0	36.0
22	35.11584327086882	36.0	36.0	36.0	36.0	36.0
23	34.99488926746167	36.0	36.0	36.0	32.0	36.0
24	35.000851788756385	36.0	36.0	36.0	32.0	36.0
25	35.05621805792163	36.0	36.0	36.0	32.0	36.0
26	34.97274275979557	36.0	36.0	36.0	32.0	36.0
27	34.811754684838164	36.0	36.0	36.0	32.0	36.0
28	35.0	36.0	36.0	36.0	36.0	36.0
29	34.40034071550256	36.0	36.0	36.0	32.0	36.0
30	34.879897785349236	36.0	36.0	36.0	32.0	36.0
31	34.84923339011925	36.0	36.0	36.0	32.0	36.0
32	34.465076660988075	36.0	36.0	36.0	32.0	36.0
33	34.5076660988075	36.0	36.0	36.0	32.0	36.0
34	34.33475298126065	36.0	36.0	36.0	32.0	36.0
35	34.17717206132879	36.0	36.0	36.0	32.0	36.0
36	34.240204429301535	36.0	36.0	36.0	32.0	36.0
37	33.799829642248724	36.0	36.0	36.0	32.0	36.0
38	33.66269165247019	36.0	36.0	36.0	27.0	36.0
39	33.73083475298126	36.0	36.0	36.0	27.0	36.0
40	33.84923339011925	36.0	36.0	36.0	32.0	36.0
41	33.47359454855196	36.0	36.0	36.0	21.0	36.0
42	34.03747870528109	36.0	36.0	36.0	32.0	36.0
43	34.057069846678026	36.0	36.0	36.0	32.0	36.0
44	33.66780238500852	36.0	36.0	36.0	27.0	36.0
45	33.91652470187393	36.0	36.0	36.0	32.0	36.0
46	33.706132879045995	36.0	36.0	36.0	27.0	36.0
47	33.54770017035775	36.0	36.0	36.0	21.0	36.0
48	33.95996592844975	36.0	36.0	36.0	32.0	36.0
49	33.69165247018739	36.0	36.0	36.0	27.0	36.0
50	33.124361158432706	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	1.0
25	1.0
26	7.0
27	12.0
28	22.0
29	26.0
30	48.0
31	45.0
32	65.0
33	78.0
34	170.0
35	697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.56388415672913	22.23168654173765	9.880749574105621	30.3236797274276
2	17.54684838160136	19.6763202725724	34.75298126064736	28.023850085178875
3	16.183986371379895	23.764906303236796	31.771720613287908	28.279386712095402
4	14.65076660988075	20.61328790459966	30.153321976149915	34.582623509369675
5	14.565587734241909	33.13458262350937	35.945485519591145	16.35434412265758
6	32.28279386712095	36.79727427597956	16.43952299829642	14.480408858603067
7	26.14991482112436	28.109028960817717	21.465076660988075	24.275979557069846
8	25.894378194207835	33.04940374787053	21.20954003407155	19.846678023850085
9	23.93526405451448	15.332197614991482	16.950596252129472	43.781942078364565
10	14.565587734241909	26.405451448040886	34.49744463373084	24.531516183986373
11	38.41567291311755	17.632027257240203	23.93526405451448	20.017035775127766
12	20.10221465076661	23.679727427597953	26.320272572402043	29.89778534923339
13	28.79045996592845	18.48381601362862	27.001703577512775	25.72402044293015
14	22.998296422487225	19.250425894378196	21.890971039182283	35.8603066439523
15	26.916524701873932	24.787052810902896	20.18739352640545	28.109028960817717
16	27.2572402044293	23.850085178875638	23.339011925042588	25.55366269165247
17	22.487223168654175	30.40885860306644	23.083475298126068	24.020442930153322
18	26.916524701873932	25.04258943781942	24.10562180579216	23.93526405451448
19	26.74616695059625	23.509369676320272	22.487223168654175	27.2572402044293
20	24.787052810902896	24.020442930153322	21.976149914821125	29.216354344122657
21	26.916524701873932	24.020442930153322	21.20954003407155	27.85349233390119
22	22.82793867120954	28.620102214650768	22.14650766609881	26.405451448040886
23	25.298126064735943	23.083475298126068	22.23168654173765	29.38671209540034
24	29.471890971039183	24.44633730834753	22.998296422487225	23.083475298126068
25	24.275979557069846	25.2129471890971	22.7427597955707	27.76831345826235
26	27.086882453151617	24.44633730834753	26.74616695059625	21.720613287904598
27	25.2129471890971	27.938671209540033	22.913117546848383	23.93526405451448
28	23.679727427597953	24.361158432708688	21.039182282793867	30.919931856899492
29	23.083475298126068	26.74616695059625	25.468483816013627	24.701873935264054
30	22.572402044293018	25.63884156729131	21.80579216354344	29.982964224872234
31	30.664395229982965	21.550255536626917	24.61669505962521	23.168654173764907
32	22.14650766609881	28.279386712095402	26.405451448040886	23.168654173764907
33	24.87223168654174	21.976149914821125	24.361158432708688	28.79045996592845
34	28.19420783645656	21.039182282793867	23.679727427597953	27.086882453151617
35	24.275979557069846	21.80579216354344	30.749574105621807	23.168654173764907
36	23.083475298126068	21.976149914821125	23.168654173764907	31.771720613287908
37	21.80579216354344	25.979557069846678	26.064735945485516	26.14991482112436
38	21.12436115843271	22.7427597955707	30.749574105621807	25.383304940374785
39	26.831345826235093	23.083475298126068	24.190800681431003	25.894378194207835
40	23.850085178875638	22.657580919931856	28.449744463373083	25.04258943781942
41	21.976149914821125	24.531516183986373	26.831345826235093	26.66098807495741
42	26.490630323679724	21.63543441226576	27.001703577512775	24.87223168654174
43	23.42419080068143	21.29471890971039	26.831345826235093	28.449744463373083
44	22.487223168654175	24.020442930153322	28.109028960817717	25.383304940374785
45	21.63543441226576	19.761499148211243	29.81260647359455	28.79045996592845
46	26.916524701873932	21.039182282793867	27.76831345826235	24.275979557069846
47	22.998296422487225	22.657580919931856	27.086882453151617	27.2572402044293
48	22.23168654173765	19.931856899488924	24.61669505962521	33.21976149914821
49	23.509369676320272	20.954003407155025	28.449744463373083	27.086882453151617
50	20.442930153321974	20.272572402044293	32.96422487223168	26.320272572402043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.5
18	1.0
19	3.0
20	5.0
21	5.5
22	6.0
23	7.0
24	8.0
25	11.0
26	14.0
27	14.5
28	15.0
29	16.0
30	17.0
31	21.0
32	25.0
33	32.0
34	39.0
35	41.0
36	43.0
37	44.5
38	46.0
39	51.5
40	57.0
41	58.0
42	59.0
43	53.5
44	48.0
45	45.0
46	42.0
47	52.5
48	63.0
49	62.5
50	62.0
51	57.0
52	52.0
53	90.5
54	129.0
55	100.0
56	71.0
57	64.0
58	57.0
59	56.0
60	55.0
61	53.0
62	51.0
63	47.5
64	44.0
65	43.0
66	42.0
67	40.0
68	38.0
69	31.0
70	24.0
71	24.5
72	25.0
73	18.0
74	11.0
75	9.5
76	8.0
77	8.0
78	8.0
79	5.0
80	2.0
81	2.5
82	3.0
83	2.5
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1174.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.80068143100512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.18386491557223	85.51959114139693
2	5.065666041275797	9.19931856899489
3	0.28142589118198874	0.7666098807495741
4	0.0	0.0
5	0.18761726078799248	0.8517887563884157
6	0.09380863039399624	0.5110732538330494
7	0.0	0.0
8	0.09380863039399624	0.6814310051107325
9	0.0	0.0
>10	0.09380863039399624	2.4701873935264054
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	29	2.4701873935264054	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	8	0.6814310051107325	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTT	6	0.5110732538330494	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT	5	0.42589437819420783	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGG	5	0.42589437819420783	No Hit
TCCCTGGACCACACATTCTCCCTTCAGGAAAGTTTTTTGCAGAAGCTGTG	3	0.2555366269165247	No Hit
GCTTCATCCGCCAGTGCGGGGCGGTGAAGACGTCCACTTCATCGGGGAAG	3	0.2555366269165247	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	3	0.2555366269165247	No Hit
GTATTACTAAGAATGGCAGAAAAGTTTTACATTCAGCATCAACCTTCACA	2	0.17035775127768313	No Hit
CCATTGGTGAAGATGACCAGGAGTTTGAGAAGGAGAATGAAGTCTTCCGA	2	0.17035775127768313	No Hit
CTGCAAAAACATGCCAAATTGTAAAGACCATCGAGGCTAGGAAGAAACTG	2	0.17035775127768313	No Hit
AGTCTGCAGCGCCTCGAGCGCCGTGTTTTCCTCCGTCGGCAGATCCCGCA	2	0.17035775127768313	No Hit
CTTCCAGAAAGTGTGGCCCAGACACTTGCTACACCACCTCTGGGACCCTG	2	0.17035775127768313	No Hit
GATTAAGGATAATTTATCAAAAAATGAAGATGTGGGTGGGGGTAGTAATT	2	0.17035775127768313	No Hit
CCCGTTGGCCGCAAGCGACCCCTGGGTCGATGCCGCGATCGACTGGGACG	2	0.17035775127768313	No Hit
TTGCCTCTACTGTGAGCAAGATGAACATGAACCTTTGTGCATAAAACCTG	2	0.17035775127768313	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTGT	2	0.17035775127768313	RNA PCR Primer, Index 7 (95% over 21bp)
AGGGACTCCTGCATGTTGACGCCGGCGGCGATGAGTCGCAGGAACATCGG	2	0.17035775127768313	No Hit
GCCCATCGTCGCCTGGGACGGCGGTTTGACGTCGGCCACGGCTTAAGCCT	2	0.17035775127768313	No Hit
CCTTCGAGCAGGTTGGAGCGCCCGCGAACAATGAGGCCTGTCTCTTATAC	2	0.17035775127768313	No Hit
GTACAGATGCAAGGGAGGGAAGAGGGCCTGGCCAGAAGGTAGGGCTAGCA	2	0.17035775127768313	No Hit
GTGGAAAGGTTCATTTGTGCTGTATATTAGATTCCAGATATAAGTGATAT	2	0.17035775127768313	No Hit
CTCTCTTTCTCTCTCTCTCACACATGCATATATGTGTGAGAGAGAGAGAA	2	0.17035775127768313	No Hit
CGCATGCGGCTAGGGGATGGTGGAGTCTCTTCTAACTTGGGTCTGACGTT	2	0.17035775127768313	No Hit
ACATTGAACTGTGCCTCGCCGTCCGACTCCGAAATGGCTAGCTCAATCAC	2	0.17035775127768313	No Hit
ATTCTCAAAAGCTCATTTGGTAAAGATTGAGGAGCACCGAATGAGCCTCA	2	0.17035775127768313	No Hit
GCTTTGCCGTCCAAGTCAGGATGTCTATTGTTGTCCTTGTTCAGCTAATG	2	0.17035775127768313	No Hit
CTACAAAGGTAAGTCTCATCCAAGTACAAAAGGGCACAAGAAAGGCAGAC	2	0.17035775127768313	No Hit
ACTATAATGCCCCTTAATGCAATGAAGCCCTGTCTCTTATACACATCTCC	2	0.17035775127768313	No Hit
ATTATAAATCGTTGCATATTTCCTATCCAAATGAACAAGATGCCGCTTAT	2	0.17035775127768313	No Hit
GAAGAAAAGTTAGTGGTTCTTTCCTTTTTCATCTAAAACTGTTTCATTTG	2	0.17035775127768313	No Hit
ACTCCATTGTGTAGATGTACCACATTTTCTGTATCCATTCCTCTGTTGAG	2	0.17035775127768313	No Hit
ATCCTGTTGCTGATCCATGCTGCCGTTGAAGGAAGATATTTAGTTCTGTT	2	0.17035775127768313	No Hit
TGTCAGAACTTCTGTGCTTTTCAGGATAAATAATATTCCACTGTATCTGT	2	0.17035775127768313	No Hit
CTGCCCACTCTGAGTATGACATTGTTCTGCTTTCTGGAAGGATTTACCTT	2	0.17035775127768313	No Hit
CGTCAACACTTGGCCTGCTGCGCCGACAACGGCACGTCGAATACCTTGTC	2	0.17035775127768313	No Hit
CACCTGTTGTGACCTTCATCTTTGTTACTGACCATGTCATATCACAAGCA	2	0.17035775127768313	No Hit
TTTTGATAGAGATTGAAATGAATCTGTAGATTGCTTTGGGTAGTACGGAC	2	0.17035775127768313	No Hit
ACTTGAGCTGGCTTGAAGGCTAGTGCTTCTGCCTGCCTGCCCTTCCCTGG	2	0.17035775127768313	No Hit
ACATAGGCTCAAAATAAAGGGATGGAGGAATATTTTCCAAGTAAAAGGAA	2	0.17035775127768313	No Hit
TCCTTCGACAGCAGCCGCGTGCGCATCGCCGAAGTGAAGCGCGGCGACCT	2	0.17035775127768313	No Hit
CACTATATTGGTGAATTTATCCTGATTGGACTAAATGAACACCTGTCTCT	2	0.17035775127768313	No Hit
AAGTCCTATTCCCTTAAAATGGAAGACCCAAAATTCTGTTTGGGTCAATC	2	0.17035775127768313	No Hit
GACATGTCCCGCAATCCTTTTATGCAAAACATGCCTGAAATCCAGGCCGC	2	0.17035775127768313	No Hit
GCTCTCGAAGGGCTCCTTTGAGGGCGTCGGCGAGGATGCCCGCCGCGTTC	2	0.17035775127768313	No Hit
AGACCCAGGAGATGGTCTTCGACGCGCACACCCGCGCCTTTGCACTGTTC	2	0.17035775127768313	No Hit
TAGCTATACTGTTCATCAATTGTCCATCTTCAGTGTCCATCAATGAGGAA	2	0.17035775127768313	No Hit
AGGCAGGGCTATGGAATTACTCTCCTGGAAAACCTTTCCAGACAGGGGTG	2	0.17035775127768313	No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	2	0.17035775127768313	RNA PCR Primer, Index 41 (95% over 22bp)
GCATGAGCCACCGCACCCGGCCCACCAAAACCTTTTACCTGTCTCTTATA	2	0.17035775127768313	No Hit
GGAAGGCGATGGTCAACGGCCCGTCCGGCAAGGTGGCAAGATGCTGCGGA	2	0.17035775127768313	No Hit
AGCATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGCTGTC	2	0.17035775127768313	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTGTT	2	0.17035775127768313	No Hit
CCCTGATATTCATGGATATACAATCTGAAAAGTTTGGCCATTCATAAGAC	2	0.17035775127768313	No Hit
GTCATTGCCAGAGTGGAAGCTAGCACCCTGATCTACTTCTGCCTCTGCAT	2	0.17035775127768313	No Hit
TGCCAAGATCGCCCTGAAGAACATTGCCACAGTAGGCGACGAACTGCGCC	2	0.17035775127768313	No Hit
ACATAGTATTCCCCCCTCCACCTGGAGCTGCCCTGGGGCTGTCTCTTATA	2	0.17035775127768313	No Hit
GTATAACATTGGAAATGTAAATGAGCTAAATACCTAATAAAAAATGGAAA	2	0.17035775127768313	No Hit
GAGATGAGACCAGAACTGTGGCTCGCGGACTACCGGCTGGCCTGCCAACT	2	0.17035775127768313	No Hit
GATATGAACATGCAGATGCGGGACTTCCTGGTGTGAATGCTGGCCGGAAT	2	0.17035775127768313	No Hit
GCTTAGCATAATGTCCTCCAGATCCATCTATGTTGAAAATGGCCCAGTTT	2	0.17035775127768313	No Hit
GGCCCATGGCGATACCGGCAACCGGACGTGCGAGCGGAACGCCTGCGTCC	2	0.17035775127768313	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.08517887563884156	0.0
21	0.0	0.0	0.0	0.08517887563884156	0.0
22	0.0	0.0	0.0	0.08517887563884156	0.0
23	0.0	0.0	0.0	0.08517887563884156	0.0
24	0.0	0.0	0.0	0.17035775127768313	0.0
25	0.0	0.0	0.0	0.17035775127768313	0.0
26	0.0	0.0	0.0	0.17035775127768313	0.0
27	0.0	0.0	0.0	0.17035775127768313	0.0
28	0.0	0.0	0.0	0.2555366269165247	0.0
29	0.0	0.0	0.0	0.2555366269165247	0.0
30	0.0	0.0	0.0	0.6814310051107325	0.0
31	0.0	0.0	0.0	1.362862010221465	0.0
32	0.0	0.0	0.0	1.5332197614991483	0.0
33	0.0	0.0	0.0	2.1294718909710393	0.0
34	0.0	0.0	0.0	2.810902896081772	0.0
35	0.0	0.0	0.0	3.4071550255536627	0.0
36	0.0	0.0	0.0	4.34412265758092	0.0
37	0.0	0.0	0.0	5.1107325383304945	0.0
38	0.0	0.0	0.0	6.473594548551959	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE