FastQCFastQC Report
Thu 2 Feb 2017
SRR4062677_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062677_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055519
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG233642.213508236232602No Hit
TCCATGTACTCTGCGTTGATACCAC206241.9539202989240365No Hit
GAGTACATGGAAGCAGTGGTATCAA94840.8985153275308166No Hit
CATGTACTCTGCGTTGATACCACTG82870.7851114001737534No Hit
GCTTCCATGTACTCTGCGTTGATAC68000.6442328371161485No Hit
CCCATGTACTCTGCGTTGATACCAC63390.6005576403645979No Hit
GCGTTGATACCACTGCTTCCATGTA56150.5319657912363491No Hit
CATGGAAGCAGTGGTATCAACGCAG54660.5178495128936571No Hit
GTACATGGGAAGCAGTGGTATCAAC54240.5138704277232338No Hit
GTATCAACGCAGAGTACATGGAAGC52340.4958698043332237No Hit
ACTCTGCGTTGATACCACTGCTTCC37540.35565442213735615No Hit
ACGCAGAGTACATGGAAGCAGTGGT35960.34068548268671617No Hit
GTATCAACGCAGAGTACTTTTTTTT34630.32808504631370916No Hit
CAGTGGTATCAACGCAGAGTACATG33300.31548460994070215No Hit
TATCAACGCAGAGTACATGGAAGCA32020.3033578741832217No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30690.2907574378102147No Hit
GTATCAACGCAGAGTACATGGGAAG25490.2414925737954504No Hit
ATACCACTGCTTCCATGTACTCTGC23140.21922864486570112No Hit
TATCAACGCAGAGTACTTTTTTTTT23140.21922864486570112No Hit
GGTATCAACGCAGAGTACATGGAAG22130.20965989243206423No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18710.17725877033004617No Hit
GAGTACATGGGAAGCAGTGGTATCA18500.17526922774483453No Hit
AAGCAGTGGTATCAACGCAGAGTAC18470.1749850073755186No Hit
GTACTCTGCGTTGATACCACTGCTT17540.1661741759267242No Hit
GCGTTGATACCACTGCTTCCCATGT16760.15878444632450955No Hit
TATCAACGCAGAGTACATGGGAAGC16710.158310745708983No Hit
GTGGTATCAACGCAGAGTACATGGA16640.15764756484724576No Hit
GCTTCCCATGTACTCTGCGTTGATA16400.15537380189271816No Hit
AAAAAGTACTCTGCGTTGATACCAC16350.15490010127719162No Hit
CCACTGCTTCCATGTACTCTGCGTT15960.15120523647608428No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14670.1389837605954985No Hit
ACATGGAAGCAGTGGTATCAACGCA14410.13652051739476032No Hit
GAAGCAGTGGTATCAACGCAGAGTA13910.1317835112394945No Hit
GGTATCAACGCAGAGTACTTTTTTT13400.1269517649611234No Hit
CTGCTTCCATGTACTCTGCGTTGAT13200.12505696249901707No Hit
GGTATCAACGCAGAGTACATGGGAA13100.12410956126796391No Hit
ACGCAGAGTACATGGGAAGCAGTGG12640.11975151560511937No Hit
GTCCTAAAGTGTGTATTTCTCATTT12620.11956203535890873No Hit
GTACATGGGGTGGTATCAACGCAAA12350.11700405203506521No Hit
CTGTAGGACGTGGAATATGGCAAGA12260.11615139092711736No Hit
GATACCACTGCTTCCATGTACTCTG12180.11539346994227484No Hit
TGGAAGCAGTGGTATCAACGCAGAG11980.11349866748016851No Hit
GTCCTACAGTGGACATTTCTAAATT11910.1128354866184313No Hit
GTTGATACCACTGCTTCCATGTACT11360.10762477984763894No Hit
CTTTAGGACGTGAAATATGGCGAGG10810.10241407307684655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCT551.12967755E-515.5424674
TCGCGTA755.8258593E-815.20284759
TCGGCGT902.2791937E-914.77984413
CGGTCGG902.2791937E-914.77984410
GTCGGCG903.6254278E-813.724792512
AAGTCGT500.001497946313.3012297
CTAATCT500.001501162713.2974444
CCGCCCC802.0019797E-613.0599894
GGTCGGC957.368908E-813.00120111
GCGGTCG1102.733941E-912.9569729
CGCCCCT751.4782736E-512.6660345
AGAATTT1600.012.46931117
CGGCGTC1103.7982318E-812.09317414
GGCGTCC1103.7982318E-812.09317415
GCGACGA550.00306410912.092613
GTGTTAC550.003079385512.0845731
CGACGGG951.0363601E-612.00224714
CGCGTAA951.0369549E-612.00167810
AGGACCT5550.011.9813845
CGTCCCC1353.7107384E-1011.96350117
Produced by FastQC (version 0.11.5)