##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062677_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1055519
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.854092631207966	32.0	32.0	32.0	32.0	32.0
2	30.542045192933525	32.0	32.0	32.0	32.0	32.0
3	30.552472290882495	32.0	32.0	32.0	32.0	32.0
4	30.570869875388315	32.0	32.0	32.0	32.0	32.0
5	30.4421976297916	32.0	32.0	32.0	32.0	32.0
6	34.013710790615804	36.0	36.0	36.0	32.0	36.0
7	33.844115548843746	36.0	36.0	36.0	32.0	36.0
8	33.812022332141815	36.0	36.0	36.0	32.0	36.0
9	33.93972159667424	36.0	36.0	36.0	32.0	36.0
10	33.64385577142619	36.0	36.0	36.0	27.0	36.0
11	34.02299248047643	36.0	36.0	36.0	32.0	36.0
12	33.7780864200455	36.0	36.0	36.0	32.0	36.0
13	33.87688426262341	36.0	36.0	36.0	32.0	36.0
14	33.76374466020981	36.0	36.0	36.0	32.0	36.0
15	33.75637387863222	36.0	36.0	36.0	32.0	36.0
16	33.728276800322874	36.0	36.0	36.0	27.0	36.0
17	33.635718542252675	36.0	36.0	36.0	27.0	36.0
18	33.68092189718991	36.0	36.0	36.0	27.0	36.0
19	33.66514671929165	36.0	36.0	36.0	27.0	36.0
20	33.62734919977755	36.0	36.0	36.0	27.0	36.0
21	33.543789358599895	36.0	36.0	36.0	27.0	36.0
22	33.51286334021462	36.0	36.0	36.0	27.0	36.0
23	33.39909276858114	36.0	36.0	36.0	27.0	36.0
24	33.42538883715026	36.0	36.0	36.0	27.0	36.0
25	32.84962942400848	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	39.0
5	112.0
6	161.0
7	54.0
8	155.0
9	194.0
10	113.0
11	37.0
12	79.0
13	65.0
14	371.0
15	401.0
16	602.0
17	869.0
18	1266.0
19	1878.0
20	2934.0
21	4321.0
22	5925.0
23	8129.0
24	10310.0
25	13135.0
26	16771.0
27	20692.0
28	26235.0
29	33334.0
30	41152.0
31	54786.0
32	75582.0
33	105696.0
34	224772.0
35	405343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.056700737894104	17.008326416760468	12.590815549213785	24.344157296131648
2	13.716521124532086	21.28038549322156	38.41772313802549	26.58537024422087
3	19.907662251900334	26.682823719791983	27.16308233370252	26.246431694605164
4	12.394348847786537	17.11198074590661	34.4530776228017	36.040592783505154
5	13.112810486381674	37.86983762636721	33.809406923506316	15.2079449637448
6	33.40486253219051	33.459931168943186	18.416620302223503	14.718585996642803
7	28.367145728262106	31.229854764703653	21.75856053998711	18.644438967047137
8	28.374000005689194	33.25248357008281	20.81647514628339	17.557041277944606
9	27.036963631898026	16.59944828135565	18.126299758279334	38.237288328466995
10	16.040364835281576	27.827037117133525	31.96595983624906	24.166638211335844
11	35.611628162858025	22.176998637917496	20.883968782131298	21.327404417093188
12	23.78004356174156	24.51182769585708	29.272978059756994	22.435150682644363
13	31.469828869965077	20.719614981134153	24.282120790753172	23.5284353581476
14	24.561932703681254	20.118986979159256	24.36928079466304	30.949799522496452
15	25.960039152752646	26.502562760117303	25.5894727656943	21.94792532143575
16	25.493100949591597	25.467777242428497	26.56836262789895	22.47075918008096
17	23.583629596184565	26.01513501618221	27.91431585097947	22.486919536653755
18	25.68785495497461	22.999801742202255	29.991566929081575	21.320776373741563
19	23.882518336944436	29.255347405886795	25.0839534186591	21.77818083850967
20	24.032997458888342	23.41493536242854	30.22907946119706	22.322987717486054
21	24.42822749099298	26.777830688934884	23.245125586001606	25.548816234070525
22	23.232190974989564	27.768606019203766	24.55985046870849	24.439352537098184
23	21.667874680287493	26.869322019748175	24.96214679832118	26.50065650164315
24	22.33821330722585	28.83434651609329	25.1105204040588	23.716919772622056
25	25.172456316758048	25.193041036635112	23.872014267013224	25.762488379593616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	596.0
1	596.0
2	952.0
3	1308.0
4	1308.0
5	1308.0
6	2941.5
7	4575.0
8	4575.0
9	4575.0
10	4287.5
11	4000.0
12	4000.0
13	4000.0
14	3656.0
15	3312.0
16	3312.0
17	3312.0
18	5102.0
19	6892.0
20	6892.0
21	6892.0
22	11213.0
23	15534.0
24	15534.0
25	15534.0
26	23818.0
27	32102.0
28	32102.0
29	32102.0
30	40685.5
31	49269.0
32	49269.0
33	49269.0
34	56861.0
35	64453.0
36	64453.0
37	64453.0
38	71090.5
39	77728.0
40	77728.0
41	77728.0
42	96077.5
43	114427.0
44	114427.0
45	114427.0
46	171473.5
47	228520.0
48	228520.0
49	228520.0
50	187239.5
51	145959.0
52	145959.0
53	145959.0
54	120530.0
55	95101.0
56	95101.0
57	95101.0
58	85714.0
59	76327.0
60	76327.0
61	76327.0
62	67274.5
63	58222.0
64	58222.0
65	58222.0
66	47591.0
67	36960.0
68	36960.0
69	36960.0
70	28020.5
71	19081.0
72	19081.0
73	19081.0
74	14542.5
75	10004.0
76	10004.0
77	10004.0
78	8233.0
79	6462.0
80	6462.0
81	6462.0
82	4442.5
83	2423.0
84	2423.0
85	2423.0
86	1816.5
87	1210.0
88	1210.0
89	1210.0
90	814.0
91	418.0
92	418.0
93	418.0
94	261.0
95	104.0
96	104.0
97	104.0
98	318.0
99	532.0
100	532.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007863430217741225
2	0.0037896049242126385
3	0.004358045662844534
4	0.015063679573745239
5	0.034106444317913744
6	0.04471733810570914
7	0.06432854358850955
8	0.08412922931752058
9	0.0943611626128947
10	0.11700405203506521
11	0.11851989400475027
12	0.13102559025465196
13	0.11681457178885458
14	0.12183579831343631
15	0.11245652612601005
16	0.11094068415632499
17	0.12079365695927785
18	0.12657280446870212
19	0.12913078779254566
20	0.11899359462027685
21	0.1270465050842287
22	0.1476998519211876
23	0.1370889581333922
24	0.13405727419402208
25	0.12685702483801806
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1055519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.82543596196235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08628911465864	33.783402271397875
2	13.534791860928902	11.863363079192576
3	4.28173101550092	5.629461852785508
4	1.759050062491579	3.083645434704423
5	0.8562020914400438	1.8761714964451934
6	0.5200027662074554	1.3673608758280873
7	0.31524217388368925	0.9670937992834596
8	0.2290950996509999	0.8032154095163424
9	0.1694969172370042	0.6685448662908323
>10	1.003638936168029	8.521399362051183
>50	0.11572978192163796	3.6576429772030745
>100	0.10922560721885	9.38395490215699
>500	0.009318851397396721	2.7446562678018767
>1k	0.008018546551248342	6.471758533047957
>5k	0.0017337397948645062	5.00046397133023
>10k+	4.3343494871612656E-4	4.177864900964391
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	23364	2.213508236232602	No Hit
TCCATGTACTCTGCGTTGATACCAC	20624	1.9539202989240365	No Hit
GAGTACATGGAAGCAGTGGTATCAA	9484	0.8985153275308166	No Hit
CATGTACTCTGCGTTGATACCACTG	8287	0.7851114001737534	No Hit
GCTTCCATGTACTCTGCGTTGATAC	6800	0.6442328371161485	No Hit
CCCATGTACTCTGCGTTGATACCAC	6339	0.6005576403645979	No Hit
GCGTTGATACCACTGCTTCCATGTA	5615	0.5319657912363491	No Hit
CATGGAAGCAGTGGTATCAACGCAG	5466	0.5178495128936571	No Hit
GTACATGGGAAGCAGTGGTATCAAC	5424	0.5138704277232338	No Hit
GTATCAACGCAGAGTACATGGAAGC	5234	0.4958698043332237	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3754	0.35565442213735615	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	3596	0.34068548268671617	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3463	0.32808504631370916	No Hit
CAGTGGTATCAACGCAGAGTACATG	3330	0.31548460994070215	No Hit
TATCAACGCAGAGTACATGGAAGCA	3202	0.3033578741832217	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3069	0.2907574378102147	No Hit
GTATCAACGCAGAGTACATGGGAAG	2549	0.2414925737954504	No Hit
ATACCACTGCTTCCATGTACTCTGC	2314	0.21922864486570112	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2314	0.21922864486570112	No Hit
GGTATCAACGCAGAGTACATGGAAG	2213	0.20965989243206423	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1871	0.17725877033004617	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1850	0.17526922774483453	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1847	0.1749850073755186	No Hit
GTACTCTGCGTTGATACCACTGCTT	1754	0.1661741759267242	No Hit
GCGTTGATACCACTGCTTCCCATGT	1676	0.15878444632450955	No Hit
TATCAACGCAGAGTACATGGGAAGC	1671	0.158310745708983	No Hit
GTGGTATCAACGCAGAGTACATGGA	1664	0.15764756484724576	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1640	0.15537380189271816	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1635	0.15490010127719162	No Hit
CCACTGCTTCCATGTACTCTGCGTT	1596	0.15120523647608428	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1467	0.1389837605954985	No Hit
ACATGGAAGCAGTGGTATCAACGCA	1441	0.13652051739476032	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1391	0.1317835112394945	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1340	0.1269517649611234	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	1320	0.12505696249901707	No Hit
GGTATCAACGCAGAGTACATGGGAA	1310	0.12410956126796391	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1264	0.11975151560511937	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1262	0.11956203535890873	No Hit
GTACATGGGGTGGTATCAACGCAAA	1235	0.11700405203506521	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1226	0.11615139092711736	No Hit
GATACCACTGCTTCCATGTACTCTG	1218	0.11539346994227484	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	1198	0.11349866748016851	No Hit
GTCCTACAGTGGACATTTCTAAATT	1191	0.1128354866184313	No Hit
GTTGATACCACTGCTTCCATGTACT	1136	0.10762477984763894	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1081	0.10241407307684655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8948024621063191E-4
2	0.0	0.0	0.0	0.0	1.8948024621063191E-4
3	0.0	0.0	0.0	0.0	1.8948024621063191E-4
4	0.0	0.0	0.0	0.0	1.8948024621063191E-4
5	0.0	0.0	0.0	0.0	1.8948024621063191E-4
6	0.0	0.0	0.0	0.0	1.8948024621063191E-4
7	0.0	0.0	0.0	0.0	1.8948024621063191E-4
8	0.0	0.0	0.0	0.0	3.7896049242126383E-4
9	0.0	0.0	0.0	9.474012310531596E-5	3.7896049242126383E-4
10	0.0	0.0	0.0	9.474012310531596E-5	3.7896049242126383E-4
11	0.0	0.0	0.0	9.474012310531596E-5	3.7896049242126383E-4
12	0.0	0.0	0.0	9.474012310531596E-5	7.579209848425277E-4
13	0.0	0.0	0.0	9.474012310531596E-5	0.0010421413541584757
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCT	55	1.12967755E-5	15.542467	4
TCGCGTA	75	5.8258593E-8	15.2028475	9
TCGGCGT	90	2.2791937E-9	14.779844	13
CGGTCGG	90	2.2791937E-9	14.779844	10
GTCGGCG	90	3.6254278E-8	13.7247925	12
AAGTCGT	50	0.0014979463	13.301229	7
CTAATCT	50	0.0015011627	13.297444	4
CCGCCCC	80	2.0019797E-6	13.059989	4
GGTCGGC	95	7.368908E-8	13.001201	11
GCGGTCG	110	2.733941E-9	12.956972	9
CGCCCCT	75	1.4782736E-5	12.666034	5
AGAATTT	160	0.0	12.469311	17
CGGCGTC	110	3.7982318E-8	12.093174	14
GGCGTCC	110	3.7982318E-8	12.093174	15
GCGACGA	55	0.003064109	12.0926	13
GTGTTAC	55	0.0030793855	12.084573	1
CGACGGG	95	1.0363601E-6	12.002247	14
CGCGTAA	95	1.0369549E-6	12.001678	10
AGGACCT	555	0.0	11.981384	5
CGTCCCC	135	3.7107384E-10	11.963501	17
>>END_MODULE